Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles
Abstract We aimed to investigate why the incidence of embryos derived from oocytes with no pronuclei (0PN) decreases using time-lapse monitoring (TLM) versus fixed-point assessment in conventional IVF cycles. We analyzed 514 embryos monitored with TLM 6–9 h after insemination and 144 embryos monitor...
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Nature Portfolio
2021
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oai:doaj.org-article:c236b6d5a7b242df9dfdec78c4a83b982021-12-02T18:48:23ZTime-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles10.1038/s41598-021-98312-12045-2322https://doaj.org/article/c236b6d5a7b242df9dfdec78c4a83b982021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98312-1https://doaj.org/toc/2045-2322Abstract We aimed to investigate why the incidence of embryos derived from oocytes with no pronuclei (0PN) decreases using time-lapse monitoring (TLM) versus fixed-point assessment in conventional IVF cycles. We analyzed 514 embryos monitored with TLM 6–9 h after insemination and 144 embryos monitored using microscopic assessment 18–21 h after insemination. The primary endpoint of this study was the incidence of 0PN-derived embryos in short insemination followed by TLM. The secondary endpoint was the duration of insemination. As exploratory endpoints, we analyzed the blastulation rate and cryo-warmed blastocyst transfer outcome of embryos with early PN fading, whereby PN disappeared within < 20 h following the initiation of insemination. The incidence of 0PN-derived embryo reduced more significantly through TLM than through fixed-point observation. The microscopic assessment time was more significantly delayed in the 0PN-derived embryo than that in the 2PN-derived embryo. The embryo with early PN fading formed good-quality blastocysts, and their pregnancy outcomes were similar to those of other embryos. Most 0PN-derived embryos in the fixed-point assessment might have resulted from missed observation of PN appearance in the early-cleaved embryos. TLM or strict laboratory schedule management may reduce 0PN-derived embryos by reducing missed PN observations.Tatsuya KobayashiHiroshi IshikawaKumiko IshiiAsuka SatoNatsuko NakamuraYoshiko SaitoHisataka HasegawaMaki FujitaAkira MitsuhashiMakio ShozuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021) |
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Medicine R Science Q Tatsuya Kobayashi Hiroshi Ishikawa Kumiko Ishii Asuka Sato Natsuko Nakamura Yoshiko Saito Hisataka Hasegawa Maki Fujita Akira Mitsuhashi Makio Shozu Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| description |
Abstract We aimed to investigate why the incidence of embryos derived from oocytes with no pronuclei (0PN) decreases using time-lapse monitoring (TLM) versus fixed-point assessment in conventional IVF cycles. We analyzed 514 embryos monitored with TLM 6–9 h after insemination and 144 embryos monitored using microscopic assessment 18–21 h after insemination. The primary endpoint of this study was the incidence of 0PN-derived embryos in short insemination followed by TLM. The secondary endpoint was the duration of insemination. As exploratory endpoints, we analyzed the blastulation rate and cryo-warmed blastocyst transfer outcome of embryos with early PN fading, whereby PN disappeared within < 20 h following the initiation of insemination. The incidence of 0PN-derived embryo reduced more significantly through TLM than through fixed-point observation. The microscopic assessment time was more significantly delayed in the 0PN-derived embryo than that in the 2PN-derived embryo. The embryo with early PN fading formed good-quality blastocysts, and their pregnancy outcomes were similar to those of other embryos. Most 0PN-derived embryos in the fixed-point assessment might have resulted from missed observation of PN appearance in the early-cleaved embryos. TLM or strict laboratory schedule management may reduce 0PN-derived embryos by reducing missed PN observations. |
| format |
article |
| author |
Tatsuya Kobayashi Hiroshi Ishikawa Kumiko Ishii Asuka Sato Natsuko Nakamura Yoshiko Saito Hisataka Hasegawa Maki Fujita Akira Mitsuhashi Makio Shozu |
| author_facet |
Tatsuya Kobayashi Hiroshi Ishikawa Kumiko Ishii Asuka Sato Natsuko Nakamura Yoshiko Saito Hisataka Hasegawa Maki Fujita Akira Mitsuhashi Makio Shozu |
| author_sort |
Tatsuya Kobayashi |
| title |
Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| title_short |
Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| title_full |
Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| title_fullStr |
Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| title_full_unstemmed |
Time-lapse monitoring of fertilized human oocytes focused on the incidence of 0PN embryos in conventional in vitro fertilization cycles |
| title_sort |
time-lapse monitoring of fertilized human oocytes focused on the incidence of 0pn embryos in conventional in vitro fertilization cycles |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c236b6d5a7b242df9dfdec78c4a83b98 |
| work_keys_str_mv |
AT tatsuyakobayashi timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT hiroshiishikawa timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT kumikoishii timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT asukasato timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT natsukonakamura timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT yoshikosaito timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT hisatakahasegawa timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT makifujita timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT akiramitsuhashi timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles AT makioshozu timelapsemonitoringoffertilizedhumanoocytesfocusedontheincidenceof0pnembryosinconventionalinvitrofertilizationcycles |
| _version_ |
1718377648054337536 |