A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)

Due to problems such as unbalanced intake of nutrients or excessive intake of energy, cultured fish accumulate fat in places such as the abdominal cavity, liver, and muscle, resulting in fatty liver, reducing the quality of fish meat, and even causing many fish deaths, resulting in losses to aquacul...

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Autores principales:	Xin Wang, Guangxin Wang, Yuan Xiao, Yanxia Zuo, Fang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	micro-computed tomography zebrafish three-dimensional imaging fat content fat distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c23aa270875148d4892b099e4b62c4ce
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spelling	oai:doaj.org-article:c23aa270875148d4892b099e4b62c4ce2021-11-25T16:30:15ZA Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)10.3390/app1122105102076-3417https://doaj.org/article/c23aa270875148d4892b099e4b62c4ce2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/10510https://doaj.org/toc/2076-3417Due to problems such as unbalanced intake of nutrients or excessive intake of energy, cultured fish accumulate fat in places such as the abdominal cavity, liver, and muscle, resulting in fatty liver, reducing the quality of fish meat, and even causing many fish deaths, resulting in losses to aquaculture production. Therefore, research on lipid metabolism in fish is important and has attracted increasing attention. The detection of fish body fat distribution and content is a key to such research. The existing methods for detecting fat distribution and content in fish have limitations, such as cumbersome procedures and damage to fish tissues, and thus, is imperative to develop a simple, fast, nondestructive fat detection technology. Taking zebrafish as the research material, this study established an imaging technology for the rapid and nondestructive detection of the fat distribution and content of fish by micro-computed tomography (micro-CT), optimized the fat CT-scanning method, determined the steps of fat quantitation in the CTAn data processing software, and constructed a three-dimensional (3D) model of zebrafish adipose tissue. This technology reveals the distribution of fish adipose tissue in an all-round way, and thus, it will play an important role in the study of lipid metabolism in fish.Xin WangGuangxin WangYuan XiaoYanxia ZuoFang ZhouMDPI AGarticlemicro-computed tomographyzebrafishthree-dimensional imagingfat contentfat distributionTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10510, p 10510 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	micro-computed tomography zebrafish three-dimensional imaging fat content fat distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	micro-computed tomography zebrafish three-dimensional imaging fat content fat distribution Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Xin Wang Guangxin Wang Yuan Xiao Yanxia Zuo Fang Zhou A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
description	Due to problems such as unbalanced intake of nutrients or excessive intake of energy, cultured fish accumulate fat in places such as the abdominal cavity, liver, and muscle, resulting in fatty liver, reducing the quality of fish meat, and even causing many fish deaths, resulting in losses to aquaculture production. Therefore, research on lipid metabolism in fish is important and has attracted increasing attention. The detection of fish body fat distribution and content is a key to such research. The existing methods for detecting fat distribution and content in fish have limitations, such as cumbersome procedures and damage to fish tissues, and thus, is imperative to develop a simple, fast, nondestructive fat detection technology. Taking zebrafish as the research material, this study established an imaging technology for the rapid and nondestructive detection of the fat distribution and content of fish by micro-computed tomography (micro-CT), optimized the fat CT-scanning method, determined the steps of fat quantitation in the CTAn data processing software, and constructed a three-dimensional (3D) model of zebrafish adipose tissue. This technology reveals the distribution of fish adipose tissue in an all-round way, and thus, it will play an important role in the study of lipid metabolism in fish.
format	article
author	Xin Wang Guangxin Wang Yuan Xiao Yanxia Zuo Fang Zhou
author_facet	Xin Wang Guangxin Wang Yuan Xiao Yanxia Zuo Fang Zhou
author_sort	Xin Wang
title	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
title_short	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
title_full	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
title_fullStr	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
title_full_unstemmed	A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)
title_sort	nondestructive method of measuring zebrafish adipose tissue based on micro-computed tomography (micro-ct)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c23aa270875148d4892b099e4b62c4ce
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A Nondestructive Method of Measuring Zebrafish Adipose Tissue Based on Micro-Computed Tomography (Micro-CT)

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