Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).

<h4>Background</h4>DNA barcoding, the analysis of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene, has been shown to provide an efficient method for the identification of species in a wide range of animal taxa. In order to assess the effect...

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Autores principales:	Doo-Sang Park, Robert Foottit, Eric Maw, Paul D N Hebert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c23feb3e580a41e68e0c7d0e35a7b10c
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spelling	oai:doaj.org-article:c23feb3e580a41e68e0c7d0e35a7b10c2021-11-18T06:55:39ZBarcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).1932-620310.1371/journal.pone.0018749https://doaj.org/article/c23feb3e580a41e68e0c7d0e35a7b10c2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21526211/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>DNA barcoding, the analysis of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene, has been shown to provide an efficient method for the identification of species in a wide range of animal taxa. In order to assess the effectiveness of barcodes in the discrimination of Heteroptera, we examined 344 species belonging to 178 genera, drawn from specimens in the Canadian National Collection of Insects.<h4>Methodology/principal findings</h4>Analysis of the COI gene revealed less than 2% intra-specific divergence in 90% of the taxa examined, while minimum interspecific distances exceeded 3% in 77% of congeneric species pairs. Instances where barcodes fail to distinguish species represented clusters of morphologically similar species, except one case of barcode identity between species in different genera. Several instances of deep intraspecific divergence were detected suggesting possible cryptic species.<h4>Conclusions/significance</h4>Although this analysis encompasses 0.8% of the described global fauna, our results indicate that DNA barcodes will aid the identification of Heteroptera. This advance will be useful in pest management, regulatory and environmental applications and will also reveal species that require further taxonomic research.Doo-Sang ParkRobert FoottitEric MawPaul D N HebertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18749 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Doo-Sang Park Robert Foottit Eric Maw Paul D N Hebert Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
description	<h4>Background</h4>DNA barcoding, the analysis of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene, has been shown to provide an efficient method for the identification of species in a wide range of animal taxa. In order to assess the effectiveness of barcodes in the discrimination of Heteroptera, we examined 344 species belonging to 178 genera, drawn from specimens in the Canadian National Collection of Insects.<h4>Methodology/principal findings</h4>Analysis of the COI gene revealed less than 2% intra-specific divergence in 90% of the taxa examined, while minimum interspecific distances exceeded 3% in 77% of congeneric species pairs. Instances where barcodes fail to distinguish species represented clusters of morphologically similar species, except one case of barcode identity between species in different genera. Several instances of deep intraspecific divergence were detected suggesting possible cryptic species.<h4>Conclusions/significance</h4>Although this analysis encompasses 0.8% of the described global fauna, our results indicate that DNA barcodes will aid the identification of Heteroptera. This advance will be useful in pest management, regulatory and environmental applications and will also reveal species that require further taxonomic research.
format	article
author	Doo-Sang Park Robert Foottit Eric Maw Paul D N Hebert
author_facet	Doo-Sang Park Robert Foottit Eric Maw Paul D N Hebert
author_sort	Doo-Sang Park
title	Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
title_short	Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
title_full	Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
title_fullStr	Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
title_full_unstemmed	Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).
title_sort	barcoding bugs: dna-based identification of the true bugs (insecta: hemiptera: heteroptera).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c23feb3e580a41e68e0c7d0e35a7b10c
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Barcoding bugs: DNA-based identification of the true bugs (Insecta: Hemiptera: Heteroptera).

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