Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.

Compelling evidence suggests that the early interaction between porcine circovirus 2 (PCV-2) and the innate immune system is the key event in the pathogenesis of Post-Weaning Multisystemic Wasting Syndrome (PMWS). Furthermore, PCV2 has been detected in bone-marrow samples, potentially enabling an ea...

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Autores principales:	Bettina Mavrommatis, Victoria Offord, Robert Patterson, Mick Watson, Theo Kanellos, Falko Steinbach, Sylvia Grierson, Dirk Werling
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c242f3504bf141a0a9edf7995839012f
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spelling	oai:doaj.org-article:c242f3504bf141a0a9edf7995839012f2021-11-18T08:28:40ZGlobal gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.1932-620310.1371/journal.pone.0091081https://doaj.org/article/c242f3504bf141a0a9edf7995839012f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618842/?tool=EBIhttps://doaj.org/toc/1932-6203Compelling evidence suggests that the early interaction between porcine circovirus 2 (PCV-2) and the innate immune system is the key event in the pathogenesis of Post-Weaning Multisystemic Wasting Syndrome (PMWS). Furthermore, PCV2 has been detected in bone-marrow samples, potentially enabling an easy spread and reservoir for the virus. To assess the gene-expression differences induced by an in-vitro PCV2b infection in different three different myeloid innate immune cell subsets generated from the same animal, we used the Agilent Porcine Gene Expression Microarray (V2). Alveolar macrophages (AMØs), monocyte-derived dendritic cells (MoDCs) and bone-marrow cells (BMCs) were generated from each animal, and challenged with a UK-isolate of a PCV2 genotype b-strain at a MOI of 0.5. Remarkably, analysis showed a highly distinct and cell-type dependent response to PCV2b challenge. Overall, MoDCs showed the most marked response to PCV2b challenge in vitro and revealed a key role for TNF in the interaction with PCV2b, whereas only few genes were affected in BMCs and AMØs. These observations were further supported by an enrichment of genes in the downstream NF-κB Signalling pathway as well as an up regulation of genes with pro-apoptotic functions post-challenge. PCV2b challenge increases the expression of a large number of immune-related and pro-apoptotic genes mainly in MoDC, which possibly explain the increased inflammation, granulomatous inflammation and lymphocyte depletion seen in PMWS-affected pigs.Bettina MavrommatisVictoria OffordRobert PattersonMick WatsonTheo KanellosFalko SteinbachSylvia GriersonDirk WerlingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91081 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bettina Mavrommatis Victoria Offord Robert Patterson Mick Watson Theo Kanellos Falko Steinbach Sylvia Grierson Dirk Werling Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
description	Compelling evidence suggests that the early interaction between porcine circovirus 2 (PCV-2) and the innate immune system is the key event in the pathogenesis of Post-Weaning Multisystemic Wasting Syndrome (PMWS). Furthermore, PCV2 has been detected in bone-marrow samples, potentially enabling an easy spread and reservoir for the virus. To assess the gene-expression differences induced by an in-vitro PCV2b infection in different three different myeloid innate immune cell subsets generated from the same animal, we used the Agilent Porcine Gene Expression Microarray (V2). Alveolar macrophages (AMØs), monocyte-derived dendritic cells (MoDCs) and bone-marrow cells (BMCs) were generated from each animal, and challenged with a UK-isolate of a PCV2 genotype b-strain at a MOI of 0.5. Remarkably, analysis showed a highly distinct and cell-type dependent response to PCV2b challenge. Overall, MoDCs showed the most marked response to PCV2b challenge in vitro and revealed a key role for TNF in the interaction with PCV2b, whereas only few genes were affected in BMCs and AMØs. These observations were further supported by an enrichment of genes in the downstream NF-κB Signalling pathway as well as an up regulation of genes with pro-apoptotic functions post-challenge. PCV2b challenge increases the expression of a large number of immune-related and pro-apoptotic genes mainly in MoDC, which possibly explain the increased inflammation, granulomatous inflammation and lymphocyte depletion seen in PMWS-affected pigs.
format	article
author	Bettina Mavrommatis Victoria Offord Robert Patterson Mick Watson Theo Kanellos Falko Steinbach Sylvia Grierson Dirk Werling
author_facet	Bettina Mavrommatis Victoria Offord Robert Patterson Mick Watson Theo Kanellos Falko Steinbach Sylvia Grierson Dirk Werling
author_sort	Bettina Mavrommatis
title	Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
title_short	Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
title_full	Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
title_fullStr	Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
title_full_unstemmed	Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
title_sort	global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c242f3504bf141a0a9edf7995839012f
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Global gene expression profiling of myeloid immune cell subsets in response to in vitro challenge with porcine circovirus 2b.

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