PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO

Much attention of researchers is directed to study the role of factors of homeostatic proliferation in the pathogenesis of autoimmune diseases, however, the effect of IL-7 and IL-15 on the phenotype of naive T cells has not yet been sufficiently investigated in the pathology. The aim of this study...

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Autores principales:	E. A. Blinova, L. V. Grishina, A. E. Sizkov, V. A. Kozlov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	naive t-cells cd31 homeostatic proliferation il-7 il-15 rheumatoid arthritis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c2433fc6dc3949598c935ed520c9f335
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spelling	oai:doaj.org-article:c2433fc6dc3949598c935ed520c9f3352021-11-18T08:03:51ZPROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO1563-06252313-741X10.15789/1563-0625-PON-2223https://doaj.org/article/c2433fc6dc3949598c935ed520c9f3352021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2223https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XMuch attention of researchers is directed to study the role of factors of homeostatic proliferation in the pathogenesis of autoimmune diseases, however, the effect of IL-7 and IL-15 on the phenotype of naive T cells has not yet been sufficiently investigated in the pathology. The aim of this study was to investigate the proportion of CD45RA+ and CD31+ naive cells among CD4+ and CD8+ lymphocytes from healthy individuals and patients with rheumatoid arthritis (RA) upon stimulation with IL-7, IL-15 in vitro. In peripheral blood, we did not find any differences in the number of CD45RA+ and CD31+ cells between the group of donors and group of RA patients. In donors, stimulation by a combination of IL-7 with IL-15 promoted an increase in the proportion of CD4+CD45RA+ and CD8+CD45RA+ relative to their level in the peripheral blood. Whereas in RA patients the number of CD8+CD45RA+ cells decreased under IL-15 and the combination of IL-15 with IL-7 compared to the control without stimulation, and it, as a proportion of CD4+CD45RA+ cells, significantly differed from the content of these cells under the same conditions in donors. There were no significant differences in the content of CD31+ cells and the number of CD31+ cells proliferating to cytokines, both between the groups of donors and patients with RA, and between different culture conditions. Thus, we can say that under the factors of homeostatic proliferation, there is a proportional increase in CD31+ T cells number, both in donors and in patients with RA. At the same time, naive T cells from donors retain the expression of CD45RA during cultivation, while naive T cells from patients partially lose it. The obtained data indicate that in RA, under factors of homeostatic proliferation the phenotype of naive T cells is converted into the phenotype of memory T cells.E. A. BlinovaL. V. GrishinaA. E. SizkovV. A. KozlovSPb RAACIarticlenaive t-cellscd31homeostatic proliferationil-7il-15rheumatoid arthritisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 725-730 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	naive t-cells cd31 homeostatic proliferation il-7 il-15 rheumatoid arthritis Immunologic diseases. Allergy RC581-607
spellingShingle	naive t-cells cd31 homeostatic proliferation il-7 il-15 rheumatoid arthritis Immunologic diseases. Allergy RC581-607 E. A. Blinova L. V. Grishina A. E. Sizkov V. A. Kozlov PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
description	Much attention of researchers is directed to study the role of factors of homeostatic proliferation in the pathogenesis of autoimmune diseases, however, the effect of IL-7 and IL-15 on the phenotype of naive T cells has not yet been sufficiently investigated in the pathology. The aim of this study was to investigate the proportion of CD45RA+ and CD31+ naive cells among CD4+ and CD8+ lymphocytes from healthy individuals and patients with rheumatoid arthritis (RA) upon stimulation with IL-7, IL-15 in vitro. In peripheral blood, we did not find any differences in the number of CD45RA+ and CD31+ cells between the group of donors and group of RA patients. In donors, stimulation by a combination of IL-7 with IL-15 promoted an increase in the proportion of CD4+CD45RA+ and CD8+CD45RA+ relative to their level in the peripheral blood. Whereas in RA patients the number of CD8+CD45RA+ cells decreased under IL-15 and the combination of IL-15 with IL-7 compared to the control without stimulation, and it, as a proportion of CD4+CD45RA+ cells, significantly differed from the content of these cells under the same conditions in donors. There were no significant differences in the content of CD31+ cells and the number of CD31+ cells proliferating to cytokines, both between the groups of donors and patients with RA, and between different culture conditions. Thus, we can say that under the factors of homeostatic proliferation, there is a proportional increase in CD31+ T cells number, both in donors and in patients with RA. At the same time, naive T cells from donors retain the expression of CD45RA during cultivation, while naive T cells from patients partially lose it. The obtained data indicate that in RA, under factors of homeostatic proliferation the phenotype of naive T cells is converted into the phenotype of memory T cells.
format	article
author	E. A. Blinova L. V. Grishina A. E. Sizkov V. A. Kozlov
author_facet	E. A. Blinova L. V. Grishina A. E. Sizkov V. A. Kozlov
author_sort	E. A. Blinova
title	PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
title_short	PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
title_full	PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
title_fullStr	PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
title_full_unstemmed	PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO
title_sort	proportion of naive cd45ra+ and cd31+t-cells in patients with rheumatoid arthritis under stimulation with homeostatic factors in vitro
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/c2433fc6dc3949598c935ed520c9f335
work_keys_str_mv	AT eablinova proportionofnaivecd45raandcd31tcellsinpatientswithrheumatoidarthritisunderstimulationwithhomeostaticfactorsinvitro AT lvgrishina proportionofnaivecd45raandcd31tcellsinpatientswithrheumatoidarthritisunderstimulationwithhomeostaticfactorsinvitro AT aesizkov proportionofnaivecd45raandcd31tcellsinpatientswithrheumatoidarthritisunderstimulationwithhomeostaticfactorsinvitro AT vakozlov proportionofnaivecd45raandcd31tcellsinpatientswithrheumatoidarthritisunderstimulationwithhomeostaticfactorsinvitro
_version_	1718422312110260224

PROPORTION OF NAIVE CD45RA+ AND CD31+T-CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS UNDER STIMULATION WITH HOMEOSTATIC FACTORS IN VITRO

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