Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

Martin Parizek1, Timothy EL Douglas2, Katarina Novotna1, Alexander Kromka3, Mariea A Brady4, Andrea Renzing4, Eske Voss4, Marketa Jarosova3, Lukas Palatinus3, Pavel Tesarek5, Pavla Ryparova5, Vera Lisa1, Ana M dos Santos2, Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute...

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Autores principales:	Parizek M, Douglas TEL, Novotna K, Kromka A, Brady MA, Renzing A, Voss E, Jarosova M, Palatinus L, Tesarek P, Ryparova P, Lisa V, dos Santos AM, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c244383db6364b6c8f12561f387aafcc
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spelling	oai:doaj.org-article:c244383db6364b6c8f12561f387aafcc2021-12-02T03:05:26ZNanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering1176-91141178-2013https://doaj.org/article/c244383db6364b6c8f12561f387aafcc2012-04-01T00:00:00Zhttp://www.dovepress.com/nanofibrous-polylactide-co-glycolide-membranes-loaded-with-diamond-nan-a9721https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Martin Parizek1, Timothy EL Douglas2, Katarina Novotna1, Alexander Kromka3, Mariea A Brady4, Andrea Renzing4, Eske Voss4, Marketa Jarosova3, Lukas Palatinus3, Pavel Tesarek5, Pavla Ryparova5, Vera Lisa1, Ana M dos Santos2, Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Polymer Chemistry and Biomaterials Group, Ghent University, Ghent, Belgium; 3Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 4Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany; 5Czech Technical University in Prague, Faculty of Civil Engineering, Prague, Czech RepublicBackground: Nanofibrous scaffolds loaded with bioactive nanoparticles are promising materials for bone tissue engineering.Methods: In this study, composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA) and diamond nanoparticles were fabricated by an electrospinning technique. PLGA was dissolved in a mixture of methylene chloride and dimethyl formamide (2:3) at a concentration of 2.3 wt%, and nanodiamond (ND) powder was added at a concentration of 0.7 wt% (about 23 wt% in dry PLGA).Results: In the composite scaffolds, the ND particles were either arranged like beads in the central part of the fibers or formed clusters protruding from the fibers. In the PLGA-ND membranes, the fibers were thicker (diameter 270 &plusmn; 9 nm) than in pure PLGA meshes (diameter 218 &plusmn; 4 nm), but the areas of pores among these fibers were smaller than in pure PLGA samples (0.46 &plusmn; 0.02 &micro;m2 versus 1.28 &plusmn; 0.09 &micro;m2 in pure PLGA samples). The PLGA-ND membranes showed higher mechanical resistance, as demonstrated by rupture tests of load and deflection of rupture probe at failure. Both types of membranes enabled the attachment, spreading, and subsequent proliferation of human osteoblast-like MG-63 cells to a similar extent, although these values were usually lower than on polystyrene dishes. Nevertheless, the cells on both types of membranes were polygonal or spindle-like in shape, and were distributed homogeneously on the samples. From days 1&ndash;7 after seeding, their number rose continuously, and at the end of the experiment, these cells were able to create a confluent layer. At the same time, the cell viability, evaluated by a LIVE/DEAD viability/cytotoxicity kit, ranged from 92% to 97% on both types of membranes. In addition, on PLGA-ND membranes, the cells formed well developed talin-containing focal adhesion plaques. As estimated by the determination of tumor necrosis factor-alpha levels in the culture medium and concentration of intercellular adhesion molecule-1, MG-63 cells, and RAW 264.7 macrophages on these membranes did not show considerable inflammatory activity.Conclusion: This study shows that nanofibrous PLGA membranes loaded with diamond nanoparticles have interesting potential for use in bone tissue engineering.Keywords: nanofibers, nanoparticles, electrospinning, nanotechnology, regenerative medicine, human bone cellsParizek MDouglas TELNovotna KKromka ABrady MARenzing AVoss EJarosova MPalatinus LTesarek PRyparova PLisa Vdos Santos AMBacakova LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1931-1951 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Parizek M Douglas TEL Novotna K Kromka A Brady MA Renzing A Voss E Jarosova M Palatinus L Tesarek P Ryparova P Lisa V dos Santos AM Bacakova L Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
description	Martin Parizek1, Timothy EL Douglas2, Katarina Novotna1, Alexander Kromka3, Mariea A Brady4, Andrea Renzing4, Eske Voss4, Marketa Jarosova3, Lukas Palatinus3, Pavel Tesarek5, Pavla Ryparova5, Vera Lisa1, Ana M dos Santos2, Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Polymer Chemistry and Biomaterials Group, Ghent University, Ghent, Belgium; 3Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 4Department of Oral and Maxillofacial Surgery, University of Kiel, Kiel, Germany; 5Czech Technical University in Prague, Faculty of Civil Engineering, Prague, Czech RepublicBackground: Nanofibrous scaffolds loaded with bioactive nanoparticles are promising materials for bone tissue engineering.Methods: In this study, composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA) and diamond nanoparticles were fabricated by an electrospinning technique. PLGA was dissolved in a mixture of methylene chloride and dimethyl formamide (2:3) at a concentration of 2.3 wt%, and nanodiamond (ND) powder was added at a concentration of 0.7 wt% (about 23 wt% in dry PLGA).Results: In the composite scaffolds, the ND particles were either arranged like beads in the central part of the fibers or formed clusters protruding from the fibers. In the PLGA-ND membranes, the fibers were thicker (diameter 270 &plusmn; 9 nm) than in pure PLGA meshes (diameter 218 &plusmn; 4 nm), but the areas of pores among these fibers were smaller than in pure PLGA samples (0.46 &plusmn; 0.02 &micro;m2 versus 1.28 &plusmn; 0.09 &micro;m2 in pure PLGA samples). The PLGA-ND membranes showed higher mechanical resistance, as demonstrated by rupture tests of load and deflection of rupture probe at failure. Both types of membranes enabled the attachment, spreading, and subsequent proliferation of human osteoblast-like MG-63 cells to a similar extent, although these values were usually lower than on polystyrene dishes. Nevertheless, the cells on both types of membranes were polygonal or spindle-like in shape, and were distributed homogeneously on the samples. From days 1&ndash;7 after seeding, their number rose continuously, and at the end of the experiment, these cells were able to create a confluent layer. At the same time, the cell viability, evaluated by a LIVE/DEAD viability/cytotoxicity kit, ranged from 92% to 97% on both types of membranes. In addition, on PLGA-ND membranes, the cells formed well developed talin-containing focal adhesion plaques. As estimated by the determination of tumor necrosis factor-alpha levels in the culture medium and concentration of intercellular adhesion molecule-1, MG-63 cells, and RAW 264.7 macrophages on these membranes did not show considerable inflammatory activity.Conclusion: This study shows that nanofibrous PLGA membranes loaded with diamond nanoparticles have interesting potential for use in bone tissue engineering.Keywords: nanofibers, nanoparticles, electrospinning, nanotechnology, regenerative medicine, human bone cells
format	article
author	Parizek M Douglas TEL Novotna K Kromka A Brady MA Renzing A Voss E Jarosova M Palatinus L Tesarek P Ryparova P Lisa V dos Santos AM Bacakova L
author_facet	Parizek M Douglas TEL Novotna K Kromka A Brady MA Renzing A Voss E Jarosova M Palatinus L Tesarek P Ryparova P Lisa V dos Santos AM Bacakova L
author_sort	Parizek M
title	Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
title_short	Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
title_full	Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
title_fullStr	Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
title_full_unstemmed	Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
title_sort	nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/c244383db6364b6c8f12561f387aafcc
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Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering

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