Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration

Xiaoxiao Wen,1 Yudong Zheng,1 Jian Wu,2 Lu-Ning Wang,1 Zhenya Yuan,1 Jiang Peng,3 Haoye Meng3 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People’s Republic of China; 2Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese...

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Autores principales:	Wen XX, Zheng YD, Wu J, Wang LN, Yuan ZY, Peng J, Meng HY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c257570eba7e4fd78423952e3bbbda20
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spelling	oai:doaj.org-article:c257570eba7e4fd78423952e3bbbda202021-12-02T11:01:34ZImmobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration1178-2013https://doaj.org/article/c257570eba7e4fd78423952e3bbbda202015-07-01T00:00:00Zhttp://www.dovepress.com/immobilization-of-collagen-peptide-on-dialdehyde-bacterial-cellulose-n-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaoxiao Wen,1 Yudong Zheng,1 Jian Wu,2 Lu-Ning Wang,1 Zhenya Yuan,1 Jiang Peng,3 Haoye Meng3 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People’s Republic of China; 2Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Soochow, People’s Republic of China; 3Institute of Orthopedics, Chinese PLA General Hospital, Beijing, People’s Republic of China Abstract: Bacterial cellulose (BC) is an alternative nanostructured biomaterial to be utilized for a wide range of biomedical applications. Because of its low bioactivity, which restricted its practical application, collagen and collagen hydrolysate were usually composited into BC. It is necessary to develop a new method to generate covalent bonds between collagen and cellulose to improve the immobilization of collagen on BC. This study describes a facile dialdehyde BC/collagen peptide nanocomposite. BC was oxidized into dialdehyde bacterial cellulose (DBC) by regioselective oxidation, and then composited with collagen peptide (Col-p) via covalent bonds to form Schiff’s base type compounds, which was demonstrated by the results of microstructures, contact angle, Col-p content, and peptide-binding ratio. The peptide-binding ratio was further affected by the degree of oxidation, pH value, and zeta potential. In vitro desorption measurement of Col-p suggested a controlled release mechanism of the nanocomposite. Cell tests indicated that the prepared DBC/Col-p composite was bioactive and suitable for cell adhesion and attachment. This work demonstrates that the DBC/Col-p composite is a promising material for tissue engineering and regeneration. Keywords: bacterial cellulose, dialdehyde cellulose, collagen peptide, composite materials, cytoactivity Wen XXZheng YDWu JWang LNYuan ZYPeng JMeng HYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4623-4637 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wen XX Zheng YD Wu J Wang LN Yuan ZY Peng J Meng HY Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
description	Xiaoxiao Wen,1 Yudong Zheng,1 Jian Wu,2 Lu-Ning Wang,1 Zhenya Yuan,1 Jiang Peng,3 Haoye Meng3 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People’s Republic of China; 2Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Soochow, People’s Republic of China; 3Institute of Orthopedics, Chinese PLA General Hospital, Beijing, People’s Republic of China Abstract: Bacterial cellulose (BC) is an alternative nanostructured biomaterial to be utilized for a wide range of biomedical applications. Because of its low bioactivity, which restricted its practical application, collagen and collagen hydrolysate were usually composited into BC. It is necessary to develop a new method to generate covalent bonds between collagen and cellulose to improve the immobilization of collagen on BC. This study describes a facile dialdehyde BC/collagen peptide nanocomposite. BC was oxidized into dialdehyde bacterial cellulose (DBC) by regioselective oxidation, and then composited with collagen peptide (Col-p) via covalent bonds to form Schiff’s base type compounds, which was demonstrated by the results of microstructures, contact angle, Col-p content, and peptide-binding ratio. The peptide-binding ratio was further affected by the degree of oxidation, pH value, and zeta potential. In vitro desorption measurement of Col-p suggested a controlled release mechanism of the nanocomposite. Cell tests indicated that the prepared DBC/Col-p composite was bioactive and suitable for cell adhesion and attachment. This work demonstrates that the DBC/Col-p composite is a promising material for tissue engineering and regeneration. Keywords: bacterial cellulose, dialdehyde cellulose, collagen peptide, composite materials, cytoactivity
format	article
author	Wen XX Zheng YD Wu J Wang LN Yuan ZY Peng J Meng HY
author_facet	Wen XX Zheng YD Wu J Wang LN Yuan ZY Peng J Meng HY
author_sort	Wen XX
title	Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
title_short	Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
title_full	Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
title_fullStr	Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
title_full_unstemmed	Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
title_sort	immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/c257570eba7e4fd78423952e3bbbda20
work_keys_str_mv	AT wenxx immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT zhengyd immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT wuj immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT wangln immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT yuanzy immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT pengj immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration AT menghy immobilizationofcollagenpeptideondialdehydebacterialcellulosenanofibersviacovalentbondsfortissueengineeringandregeneration
_version_	1718396377158909952

Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration

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