Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

Abstract In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released...

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Autores principales:	Svetlana Sokovic Bajic, Maria-Alexandra Cañas, Maja Tolinacki, Josefa Badia, Borja Sánchez, Natasa Golic, Abelardo Margolles, Laura Baldomá, Patricia Ruas-Madiedo
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Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c25cfd1a9b03496197497b3040a8bf9a
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spelling	oai:doaj.org-article:c25cfd1a9b03496197497b3040a8bf9a2021-12-02T15:11:50ZProteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line10.1038/s41598-020-78920-z2045-2322https://doaj.org/article/c25cfd1a9b03496197497b3040a8bf9a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78920-zhttps://doaj.org/toc/2045-2322Abstract In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released by the dairy isolate Lactiplantibacillus plantarum BGAN8 and to gain an insight into the putative mechanism of EVs uptake by intestinal epithelial cells. The cryo-TEM observation undoubtedly demonstrated the release of EVs (20 to 140 nm) from the surface of BGAN8, with exopolysaccharides seems to be part of EVs surface. The proteomic analysis revealed that the EVs are enriched in enzymes involved in central metabolic pathways, such as glycolysis, and in membrane components with the most abundant proteins belonging to amino acid/peptide ABC transporters. Putative internalization pathways were evaluated in time-course internalization experiments with non-polarized HT29 cells in the presence of inhibitors of endocytic pathways: chlorpromazine and dynasore (inhibitors of clathrin-mediated endocytosis—CME) and filipin III and nystatin (disrupting lipid rafts). For the first time, our results revealed that the internalization was specifically inhibited by dynasore and chlorpromazine but not by filipin III and nystatin implying that one of the entries of L. plantarum vesicles was through CME pathway.Svetlana Sokovic BajicMaria-Alexandra CañasMaja TolinackiJosefa BadiaBorja SánchezNatasa GolicAbelardo MargollesLaura BaldomáPatricia Ruas-MadiedoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Svetlana Sokovic Bajic Maria-Alexandra Cañas Maja Tolinacki Josefa Badia Borja Sánchez Natasa Golic Abelardo Margolles Laura Baldomá Patricia Ruas-Madiedo Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
description	Abstract In recent years the role of extracellular vesicles (EVs) of Gram-positive bacteria in host-microbe cross-talk has become increasingly appreciated, although the knowledge of their biogenesis, release and host-uptake is still limited. The aim of this study was to characterize the EVs released by the dairy isolate Lactiplantibacillus plantarum BGAN8 and to gain an insight into the putative mechanism of EVs uptake by intestinal epithelial cells. The cryo-TEM observation undoubtedly demonstrated the release of EVs (20 to 140 nm) from the surface of BGAN8, with exopolysaccharides seems to be part of EVs surface. The proteomic analysis revealed that the EVs are enriched in enzymes involved in central metabolic pathways, such as glycolysis, and in membrane components with the most abundant proteins belonging to amino acid/peptide ABC transporters. Putative internalization pathways were evaluated in time-course internalization experiments with non-polarized HT29 cells in the presence of inhibitors of endocytic pathways: chlorpromazine and dynasore (inhibitors of clathrin-mediated endocytosis—CME) and filipin III and nystatin (disrupting lipid rafts). For the first time, our results revealed that the internalization was specifically inhibited by dynasore and chlorpromazine but not by filipin III and nystatin implying that one of the entries of L. plantarum vesicles was through CME pathway.
format	article
author	Svetlana Sokovic Bajic Maria-Alexandra Cañas Maja Tolinacki Josefa Badia Borja Sánchez Natasa Golic Abelardo Margolles Laura Baldomá Patricia Ruas-Madiedo
author_facet	Svetlana Sokovic Bajic Maria-Alexandra Cañas Maja Tolinacki Josefa Badia Borja Sánchez Natasa Golic Abelardo Margolles Laura Baldomá Patricia Ruas-Madiedo
author_sort	Svetlana Sokovic Bajic
title	Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
title_short	Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
title_full	Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
title_fullStr	Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
title_full_unstemmed	Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
title_sort	proteomic profile of extracellular vesicles released by lactiplantibacillus plantarum bgan8 and their internalization by non-polarized ht29 cell line
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c25cfd1a9b03496197497b3040a8bf9a
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Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

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