The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis

Abstract Coordinated reorganization of cytoskeletal structures is critical for key aspects of platelet physiology. While several studies have addressed the role of microtubules and filamentous actin in platelet production and function, the significance of their crosstalk in these processes has been...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yvonne Schurr, Markus Spindler, Hendrikje Kurz, Markus Bender
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c264abe5737d4c679b3a80504f711f3b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c264abe5737d4c679b3a80504f711f3b
record_format	dspace
spelling	oai:doaj.org-article:c264abe5737d4c679b3a80504f711f3b2021-12-02T15:09:14ZThe cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis10.1038/s41598-019-44183-62045-2322https://doaj.org/article/c264abe5737d4c679b3a80504f711f3b2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44183-6https://doaj.org/toc/2045-2322Abstract Coordinated reorganization of cytoskeletal structures is critical for key aspects of platelet physiology. While several studies have addressed the role of microtubules and filamentous actin in platelet production and function, the significance of their crosstalk in these processes has been poorly investigated. The microtubule-actin cross-linking factor 1 (MACF1; synonym: Actin cross-linking factor 7, ACF7) is a member of the spectraplakin family, and one of the few proteins expressed in platelets, which possess actin and microtubule binding domains thereby facilitating actin-microtubule interaction and regulation. We used megakaryocyte- and platelet-specific Macf1 knockout (Macf1 fl/fl , Pf4-Cre) mice to study the role of MACF1 in platelet production and function. MACF1 deficient mice displayed comparable platelet counts to control mice. Analysis of the platelet cytoskeletal ultrastructure revealed a normal marginal band and actin network. Platelet spreading on fibrinogen was slightly delayed but platelet activation and clot traction was unaffected. Ex vivo thrombus formation and mouse tail bleeding responses were similar between control and mutant mice. These results suggest that MACF1 is dispensable for thrombopoiesis, platelet activation, thrombus formation and the hemostatic function in mice.Yvonne SchurrMarkus SpindlerHendrikje KurzMarkus BenderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yvonne Schurr Markus Spindler Hendrikje Kurz Markus Bender The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
description	Abstract Coordinated reorganization of cytoskeletal structures is critical for key aspects of platelet physiology. While several studies have addressed the role of microtubules and filamentous actin in platelet production and function, the significance of their crosstalk in these processes has been poorly investigated. The microtubule-actin cross-linking factor 1 (MACF1; synonym: Actin cross-linking factor 7, ACF7) is a member of the spectraplakin family, and one of the few proteins expressed in platelets, which possess actin and microtubule binding domains thereby facilitating actin-microtubule interaction and regulation. We used megakaryocyte- and platelet-specific Macf1 knockout (Macf1 fl/fl , Pf4-Cre) mice to study the role of MACF1 in platelet production and function. MACF1 deficient mice displayed comparable platelet counts to control mice. Analysis of the platelet cytoskeletal ultrastructure revealed a normal marginal band and actin network. Platelet spreading on fibrinogen was slightly delayed but platelet activation and clot traction was unaffected. Ex vivo thrombus formation and mouse tail bleeding responses were similar between control and mutant mice. These results suggest that MACF1 is dispensable for thrombopoiesis, platelet activation, thrombus formation and the hemostatic function in mice.
format	article
author	Yvonne Schurr Markus Spindler Hendrikje Kurz Markus Bender
author_facet	Yvonne Schurr Markus Spindler Hendrikje Kurz Markus Bender
author_sort	Yvonne Schurr
title	The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
title_short	The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
title_full	The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
title_fullStr	The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
title_full_unstemmed	The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis
title_sort	cytoskeletal crosslinking protein macf1 is dispensable for thrombus formation and hemostasis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c264abe5737d4c679b3a80504f711f3b
work_keys_str_mv	AT yvonneschurr thecytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT markusspindler thecytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT hendrikjekurz thecytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT markusbender thecytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT yvonneschurr cytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT markusspindler cytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT hendrikjekurz cytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis AT markusbender cytoskeletalcrosslinkingproteinmacf1isdispensableforthrombusformationandhemostasis
_version_	1718387899914780672

The cytoskeletal crosslinking protein MACF1 is dispensable for thrombus formation and hemostasis

Ejemplares similares