Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling

Abstract Tight junctions are required for the formation of tissue barriers and function as suppressors of signalling mechanisms that control gene expression and cell behaviour; however, little is known about the physiological and developmental importance of such signalling functions. Here, we demons...

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Autores principales:	Barbara Vacca, Elena Sanchez-Heras, Emily Steed, Sophie L. Busson, Maria S. Balda, Shin-Ichi Ohnuma, Noriaki Sasai, Roberto Mayor, Karl Matter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c26610b6f3364da2a752576fb3362555
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spelling	oai:doaj.org-article:c26610b6f3364da2a752576fb33625552021-12-02T11:41:24ZControl of neural crest induction by MarvelD3-mediated attenuation of JNK signalling10.1038/s41598-018-19579-52045-2322https://doaj.org/article/c26610b6f3364da2a752576fb33625552018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19579-5https://doaj.org/toc/2045-2322Abstract Tight junctions are required for the formation of tissue barriers and function as suppressors of signalling mechanisms that control gene expression and cell behaviour; however, little is known about the physiological and developmental importance of such signalling functions. Here, we demonstrate that depletion of MarvelD3, a transmembrane protein of tight junctions, disrupts neural crest formation and, consequently, development of neural crest-derived tissues during Xenopus embryogenesis. Using embryos and explant cultures combined with a small molecule inhibitor or mutant mRNAs, we show that MarvelD3 is required to attenuate JNK signalling during neural crest induction and that inhibition of JNK pathway activation is sufficient to rescue the phenotype induced by MarvelD3 depletion. Direct JNK stimulation disrupts neural crest development, supporting the importance of negative regulation of JNK. Our data identify the junctional protein MarvelD3 as an essential regulator of early vertebrate development and neural crest induction and, thereby, link tight junctions to the control and timing of JNK signalling during early development.Barbara VaccaElena Sanchez-HerasEmily SteedSophie L. BussonMaria S. BaldaShin-Ichi OhnumaNoriaki SasaiRoberto MayorKarl MatterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Vacca Elena Sanchez-Heras Emily Steed Sophie L. Busson Maria S. Balda Shin-Ichi Ohnuma Noriaki Sasai Roberto Mayor Karl Matter Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
description	Abstract Tight junctions are required for the formation of tissue barriers and function as suppressors of signalling mechanisms that control gene expression and cell behaviour; however, little is known about the physiological and developmental importance of such signalling functions. Here, we demonstrate that depletion of MarvelD3, a transmembrane protein of tight junctions, disrupts neural crest formation and, consequently, development of neural crest-derived tissues during Xenopus embryogenesis. Using embryos and explant cultures combined with a small molecule inhibitor or mutant mRNAs, we show that MarvelD3 is required to attenuate JNK signalling during neural crest induction and that inhibition of JNK pathway activation is sufficient to rescue the phenotype induced by MarvelD3 depletion. Direct JNK stimulation disrupts neural crest development, supporting the importance of negative regulation of JNK. Our data identify the junctional protein MarvelD3 as an essential regulator of early vertebrate development and neural crest induction and, thereby, link tight junctions to the control and timing of JNK signalling during early development.
format	article
author	Barbara Vacca Elena Sanchez-Heras Emily Steed Sophie L. Busson Maria S. Balda Shin-Ichi Ohnuma Noriaki Sasai Roberto Mayor Karl Matter
author_facet	Barbara Vacca Elena Sanchez-Heras Emily Steed Sophie L. Busson Maria S. Balda Shin-Ichi Ohnuma Noriaki Sasai Roberto Mayor Karl Matter
author_sort	Barbara Vacca
title	Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
title_short	Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
title_full	Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
title_fullStr	Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
title_full_unstemmed	Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
title_sort	control of neural crest induction by marveld3-mediated attenuation of jnk signalling
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c26610b6f3364da2a752576fb3362555
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Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling

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