miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis

Abstract Drug resistance, which is closely correlated with an imbalance in apoptosis, endows colorectal cancer (CRC) with enhanced progression capacity irrespective of the treatment with therapeutics. We report that miR-15b-5p is a tumor suppressor whose level is globally decreased in CRC cells and...

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Autores principales:	Ci Zhao, Qi Zhao, Chunhui Zhang, Guangyu Wang, Yuanfei Yao, Xiaoyi Huang, Fei Zhan, Yuanyuan Zhu, Jiaqi Shi, Jianan Chen, Feihu Yan, Yanqiao Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c26aeba7297c45e4a2d7ad6e2b180596
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spelling	oai:doaj.org-article:c26aeba7297c45e4a2d7ad6e2b1805962021-12-02T11:41:12ZmiR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis10.1038/s41598-017-04172-z2045-2322https://doaj.org/article/c26aeba7297c45e4a2d7ad6e2b1805962017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04172-zhttps://doaj.org/toc/2045-2322Abstract Drug resistance, which is closely correlated with an imbalance in apoptosis, endows colorectal cancer (CRC) with enhanced progression capacity irrespective of the treatment with therapeutics. We report that miR-15b-5p is a tumor suppressor whose level is globally decreased in CRC cells and tissues. Over-expression of miR-15b-5p not only promoted 5-fluorouracil (5-FU)-induced cellular apoptosis but also reversed the chemoresistance of 5-FU in vitro and in vivo. As a key mediator of inflammation-induced cancer, miR-15b-5p enhances these therapeutic effects are mainly attributed to targeting of the NF-κB signaling pathway through negative regulation of NF-κB1 and one of its kinase complexes IKK-α. miR-15b-5p mediates NF-ĸB regulation by targeting the anti-apoptosis protein XIAP in vitro. Together, these results establish an axis of miR-15b-mediated apoptosis regulation, which reverses chemoresistance and suppresses CRC progression. These findings suggest that miR-15b-5p may be a potential agent for CRC treatment, particularly for 5-FU-resistant CRC.Ci ZhaoQi ZhaoChunhui ZhangGuangyu WangYuanfei YaoXiaoyi HuangFei ZhanYuanyuan ZhuJiaqi ShiJianan ChenFeihu YanYanqiao ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ci Zhao Qi Zhao Chunhui Zhang Guangyu Wang Yuanfei Yao Xiaoyi Huang Fei Zhan Yuanyuan Zhu Jiaqi Shi Jianan Chen Feihu Yan Yanqiao Zhang miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
description	Abstract Drug resistance, which is closely correlated with an imbalance in apoptosis, endows colorectal cancer (CRC) with enhanced progression capacity irrespective of the treatment with therapeutics. We report that miR-15b-5p is a tumor suppressor whose level is globally decreased in CRC cells and tissues. Over-expression of miR-15b-5p not only promoted 5-fluorouracil (5-FU)-induced cellular apoptosis but also reversed the chemoresistance of 5-FU in vitro and in vivo. As a key mediator of inflammation-induced cancer, miR-15b-5p enhances these therapeutic effects are mainly attributed to targeting of the NF-κB signaling pathway through negative regulation of NF-κB1 and one of its kinase complexes IKK-α. miR-15b-5p mediates NF-ĸB regulation by targeting the anti-apoptosis protein XIAP in vitro. Together, these results establish an axis of miR-15b-mediated apoptosis regulation, which reverses chemoresistance and suppresses CRC progression. These findings suggest that miR-15b-5p may be a potential agent for CRC treatment, particularly for 5-FU-resistant CRC.
format	article
author	Ci Zhao Qi Zhao Chunhui Zhang Guangyu Wang Yuanfei Yao Xiaoyi Huang Fei Zhan Yuanyuan Zhu Jiaqi Shi Jianan Chen Feihu Yan Yanqiao Zhang
author_facet	Ci Zhao Qi Zhao Chunhui Zhang Guangyu Wang Yuanfei Yao Xiaoyi Huang Fei Zhan Yuanyuan Zhu Jiaqi Shi Jianan Chen Feihu Yan Yanqiao Zhang
author_sort	Ci Zhao
title	miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
title_short	miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
title_full	miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
title_fullStr	miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
title_full_unstemmed	miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis
title_sort	mir-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the nf-κb/xiap axis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c26aeba7297c45e4a2d7ad6e2b180596
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miR-15b-5p resensitizes colon cancer cells to 5-fluorouracil by promoting apoptosis via the NF-κB/XIAP axis

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