Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) pati...

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Autores principales:	Roghayeh Nouri, Alka Hasani, Kourosh Masnadi Shirazi, Mohammad Reza Alivand, Bita Sepehri, Simin Sotoudeh, Fatemeh Hemmati, Afshin Fattahzadeh, Babak Abdinia, Mohammad Ahangarzadeh Rezaee
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c272939ecd014d0b93835723f23d007b
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spelling	oai:doaj.org-article:c272939ecd014d0b93835723f23d007b2021-11-22T01:10:28ZMucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features1918-149310.1155/2021/2131787https://doaj.org/article/c272939ecd014d0b93835723f23d007b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2131787https://doaj.org/toc/1918-1493Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group (p<0.05). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains (p<0.05). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group (p<0.05). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains (p<0.05). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients.Roghayeh NouriAlka HasaniKourosh Masnadi ShiraziMohammad Reza AlivandBita SepehriSimin SotoudehFatemeh HemmatiAfshin FattahzadehBabak AbdiniaMohammad Ahangarzadeh RezaeeHindawi LimitedarticleInfectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENCanadian Journal of Infectious Diseases and Medical Microbiology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
description	Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group (p<0.05). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains (p<0.05). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group (p<0.05). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains (p<0.05). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients.
format	article
author	Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee
author_facet	Roghayeh Nouri Alka Hasani Kourosh Masnadi Shirazi Mohammad Reza Alivand Bita Sepehri Simin Sotoudeh Fatemeh Hemmati Afshin Fattahzadeh Babak Abdinia Mohammad Ahangarzadeh Rezaee
author_sort	Roghayeh Nouri
title	Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
title_short	Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
title_full	Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
title_fullStr	Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
title_full_unstemmed	Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features
title_sort	mucosa-associated escherichia coli in colorectal cancer patients and control subjects: variations in the prevalence and attributing features
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/c272939ecd014d0b93835723f23d007b
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Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

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