Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis

Abstract Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand. Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneous...

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Autores principales:	Lanhua Liu, Xiaohong Zhou, James S. Wilkinson, Ping Hua, Baodong Song, Hanchang Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c284761e60314ea0baeb5c18c3824411
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spelling	oai:doaj.org-article:c284761e60314ea0baeb5c18c38244112021-12-02T11:52:20ZIntegrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis10.1038/s41598-017-03939-82045-2322https://doaj.org/article/c284761e60314ea0baeb5c18c38244112017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03939-8https://doaj.org/toc/2045-2322Abstract Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand. Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneously. In particular, we use waveguide tapers to improve the efficiency of excitation and collection of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay. Under the optimized waveguide geometry, this is the first demonstration of using such a type of waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water. The waveguide chip was activated by (3-Mercaptopropyl) trimethoxysilane/N-(4-maleimidobutyryloxy) succinimide (MTS/GMBS) for immobilization of BSA-MC-LR conjugate, which was confirmed to have uniform monolayer distribution by atomic force microscopy. All real lake samples, even those containing MC-LR in the sub-microgram per liter range (e.g. 0.5 μg/L), could be determined by the immunosensor with recovery rates between 84% and 108%, confirming its application potential in the measurement of MC-LR in real water samples.Lanhua LiuXiaohong ZhouJames S. WilkinsonPing HuaBaodong SongHanchang ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lanhua Liu Xiaohong Zhou James S. Wilkinson Ping Hua Baodong Song Hanchang Shi Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
description	Abstract Nowadays, biosensor technologies which can detect various contaminants in water quickly and cost-effectively are in great demand. Herein, we report an integrated channel waveguide-based fluorescent immunosensor with the ability to detect a maximum of 32 contaminants rapidly and simultaneously. In particular, we use waveguide tapers to improve the efficiency of excitation and collection of fluorescent signals in the presence of fluorophore photobleaching in a solid surface bioassay. Under the optimized waveguide geometry, this is the first demonstration of using such a type of waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water. The waveguide chip was activated by (3-Mercaptopropyl) trimethoxysilane/N-(4-maleimidobutyryloxy) succinimide (MTS/GMBS) for immobilization of BSA-MC-LR conjugate, which was confirmed to have uniform monolayer distribution by atomic force microscopy. All real lake samples, even those containing MC-LR in the sub-microgram per liter range (e.g. 0.5 μg/L), could be determined by the immunosensor with recovery rates between 84% and 108%, confirming its application potential in the measurement of MC-LR in real water samples.
format	article
author	Lanhua Liu Xiaohong Zhou James S. Wilkinson Ping Hua Baodong Song Hanchang Shi
author_facet	Lanhua Liu Xiaohong Zhou James S. Wilkinson Ping Hua Baodong Song Hanchang Shi
author_sort	Lanhua Liu
title	Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
title_short	Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
title_full	Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
title_fullStr	Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
title_full_unstemmed	Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis
title_sort	integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-lr in lakes: optimization and analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c284761e60314ea0baeb5c18c3824411
work_keys_str_mv	AT lanhualiu integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis AT xiaohongzhou integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis AT jamesswilkinson integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis AT pinghua integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis AT baodongsong integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis AT hanchangshi integratedopticalwaveguidebasedfluorescentimmunosensorforfastandsensitivedetectionofmicrocystinlrinlakesoptimizationandanalysis
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Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: Optimization and Analysis

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