Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores
Abstract This paper presents eigen-analysis of image stack of blinking fluorophores to identify the components that enable super-resolved imaging of blinking fluorophores. Eigen-analysis reveals that the contributions of spatial distribution of fluorophores and their temporal photon emission charact...
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Nature Portfolio
2017
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oai:doaj.org-article:c286e9297b334b2fa5e25aa17ff1af7c2021-12-02T15:06:14ZEigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores10.1038/s41598-017-04544-52045-2322https://doaj.org/article/c286e9297b334b2fa5e25aa17ff1af7c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04544-5https://doaj.org/toc/2045-2322Abstract This paper presents eigen-analysis of image stack of blinking fluorophores to identify the components that enable super-resolved imaging of blinking fluorophores. Eigen-analysis reveals that the contributions of spatial distribution of fluorophores and their temporal photon emission characteristics can be completely separated. While cross-emitter cross-pixel information of spatial distribution that permits super-resolution is encoded in two matrices, temporal statistics weigh the contribution of these matrices to the measured data. The properties and conditions of exploitation of these matrices are investigated. Con-temporary super-resolution imaging methods that use blinking for super-resolution are studied in the context of the presented analysis. Besides providing insight into the capabilities and limitations of existing super-resolution methods, the analysis shall help in designing better super-resolution techniques that directly exploit these matrices.Krishna AgarwalDilip K. PrasadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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DOAJ |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Krishna Agarwal Dilip K. Prasad Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| description |
Abstract This paper presents eigen-analysis of image stack of blinking fluorophores to identify the components that enable super-resolved imaging of blinking fluorophores. Eigen-analysis reveals that the contributions of spatial distribution of fluorophores and their temporal photon emission characteristics can be completely separated. While cross-emitter cross-pixel information of spatial distribution that permits super-resolution is encoded in two matrices, temporal statistics weigh the contribution of these matrices to the measured data. The properties and conditions of exploitation of these matrices are investigated. Con-temporary super-resolution imaging methods that use blinking for super-resolution are studied in the context of the presented analysis. Besides providing insight into the capabilities and limitations of existing super-resolution methods, the analysis shall help in designing better super-resolution techniques that directly exploit these matrices. |
| format |
article |
| author |
Krishna Agarwal Dilip K. Prasad |
| author_facet |
Krishna Agarwal Dilip K. Prasad |
| author_sort |
Krishna Agarwal |
| title |
Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| title_short |
Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| title_full |
Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| title_fullStr |
Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| title_full_unstemmed |
Eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| title_sort |
eigen-analysis reveals components supporting super-resolution imaging of blinking fluorophores |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/c286e9297b334b2fa5e25aa17ff1af7c |
| work_keys_str_mv |
AT krishnaagarwal eigenanalysisrevealscomponentssupportingsuperresolutionimagingofblinkingfluorophores AT dilipkprasad eigenanalysisrevealscomponentssupportingsuperresolutionimagingofblinkingfluorophores |
| _version_ |
1718388540501393408 |