Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.

Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. In rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animal...

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Autores principales:	Luciene Covolan, Antônio-Carlos G de Almeida, Beatriz Amorim, Clarissa Cavarsan, Maisa Ferreira Miranda, Mayra C Aarão, Ana Paula Madureira, Antônio M Rodrigues, José N Nobrega, Luiz E Mello, Clement Hamani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c28bd33cb4284c32aaea087987618f21
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spelling	oai:doaj.org-article:c28bd33cb4284c32aaea087987618f212021-11-18T08:17:27ZEffects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.1932-620310.1371/journal.pone.0097618https://doaj.org/article/c28bd33cb4284c32aaea087987618f212014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24892420/?tool=EBIhttps://doaj.org/toc/1932-6203Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. In rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animals have not been extensively addressed. We study the effects of anterior thalamic nucleus (AN) stimulation at different current intensities in rats rendered epileptic following pilocarpine (Pilo) administration. Four months after Pilo-induced SE, chronic epileptic rats were bilaterally implanted with AN electrodes or had sham-surgery. Stimulation was delivered for 6 h/day, 5 days/week at 130 Hz, 90 µsec. and either 100 µA or 500 µA. The frequency of spontaneous recurrent seizures in animals receiving stimulation was compared to that recorded in the preoperative period and in rats given sham treatment. To investigate the effects of DBS on hippocampal excitability, brain slices from animals receiving AN DBS or sham surgery were studied with electrophysiology. We found that rats treated with AN DBS at 100 µA had a 52% non-significant reduction in the frequency of seizures as compared to sham-treated controls and 61% less seizures than at baseline. Animals given DBS at 500 µA had 5.1 times more seizures than controls and a 2.8 fold increase in seizure rate as compared to preoperative values. In non-stimulated controls, the average frequency of seizures before and after surgery remained unaltered. In vitro recordings have shown that slices from animals previously given DBS at 100 µA had a longer latency for the development of epileptiform activity, shorter and smaller DC shifts, and a smaller spike amplitude compared to non-stimulated controls. In contrast, a higher spike amplitude was recorded in slices from animals given AN DBS at 500 µA.Luciene CovolanAntônio-Carlos G de AlmeidaBeatriz AmorimClarissa CavarsanMaisa Ferreira MirandaMayra C AarãoAna Paula MadureiraAntônio M RodriguesJosé N NobregaLuiz E MelloClement HamaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e97618 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luciene Covolan Antônio-Carlos G de Almeida Beatriz Amorim Clarissa Cavarsan Maisa Ferreira Miranda Mayra C Aarão Ana Paula Madureira Antônio M Rodrigues José N Nobrega Luiz E Mello Clement Hamani Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
description	Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. In rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animals have not been extensively addressed. We study the effects of anterior thalamic nucleus (AN) stimulation at different current intensities in rats rendered epileptic following pilocarpine (Pilo) administration. Four months after Pilo-induced SE, chronic epileptic rats were bilaterally implanted with AN electrodes or had sham-surgery. Stimulation was delivered for 6 h/day, 5 days/week at 130 Hz, 90 µsec. and either 100 µA or 500 µA. The frequency of spontaneous recurrent seizures in animals receiving stimulation was compared to that recorded in the preoperative period and in rats given sham treatment. To investigate the effects of DBS on hippocampal excitability, brain slices from animals receiving AN DBS or sham surgery were studied with electrophysiology. We found that rats treated with AN DBS at 100 µA had a 52% non-significant reduction in the frequency of seizures as compared to sham-treated controls and 61% less seizures than at baseline. Animals given DBS at 500 µA had 5.1 times more seizures than controls and a 2.8 fold increase in seizure rate as compared to preoperative values. In non-stimulated controls, the average frequency of seizures before and after surgery remained unaltered. In vitro recordings have shown that slices from animals previously given DBS at 100 µA had a longer latency for the development of epileptiform activity, shorter and smaller DC shifts, and a smaller spike amplitude compared to non-stimulated controls. In contrast, a higher spike amplitude was recorded in slices from animals given AN DBS at 500 µA.
format	article
author	Luciene Covolan Antônio-Carlos G de Almeida Beatriz Amorim Clarissa Cavarsan Maisa Ferreira Miranda Mayra C Aarão Ana Paula Madureira Antônio M Rodrigues José N Nobrega Luiz E Mello Clement Hamani
author_facet	Luciene Covolan Antônio-Carlos G de Almeida Beatriz Amorim Clarissa Cavarsan Maisa Ferreira Miranda Mayra C Aarão Ana Paula Madureira Antônio M Rodrigues José N Nobrega Luiz E Mello Clement Hamani
author_sort	Luciene Covolan
title	Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
title_short	Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
title_full	Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
title_fullStr	Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
title_full_unstemmed	Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
title_sort	effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c28bd33cb4284c32aaea087987618f21
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Effects of anterior thalamic nucleus deep brain stimulation in chronic epileptic rats.

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