Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production

ABSTRACT Lipids from microbes offer a promising source of renewable alternatives to petroleum-derived compounds. In particular, oleaginous microbes are of interest because they accumulate a large fraction of their biomass as lipids. In this study, we analyzed genetic changes that alter lipid accumul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kimberly C. Lemmer, Weiping Zhang, Samantha J. Langer, Alice C. Dohnalkova, Dehong Hu, Rachelle A. Lemke, Jeff S. Piotrowski, Galya Orr, Daniel R. Noguera, Timothy J. Donohue
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Rhodobacter bioreactors cell envelope fatty acids lipid synthesis two-component regulatory systems Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c291e532b948447ebf35d48997c96929
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c291e532b948447ebf35d48997c96929
record_format	dspace
spelling	oai:doaj.org-article:c291e532b948447ebf35d48997c969292021-11-15T15:51:30ZMutations That Alter the Bacterial Cell Envelope Increase Lipid Production10.1128/mBio.00513-172150-7511https://doaj.org/article/c291e532b948447ebf35d48997c969292017-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00513-17https://doaj.org/toc/2150-7511ABSTRACT Lipids from microbes offer a promising source of renewable alternatives to petroleum-derived compounds. In particular, oleaginous microbes are of interest because they accumulate a large fraction of their biomass as lipids. In this study, we analyzed genetic changes that alter lipid accumulation in Rhodobacter sphaeroides. By screening an R. sphaeroides Tn5 mutant library for insertions that increased fatty acid content, we identified 10 high-lipid (HL) mutants for further characterization. These HL mutants exhibited increased sensitivity to drugs that target the bacterial cell envelope and changes in shape, and some had the ability to secrete lipids, with two HL mutants accumulating ~60% of their total lipids extracellularly. When one of the highest-lipid-secreting strains was grown in a fed-batch bioreactor, its lipid content was comparable to that of oleaginous microbes, with the majority of the lipids secreted into the medium. Based on the properties of these HL mutants, we conclude that alterations of the cell envelope are a previously unreported approach to increase microbial lipid production. We also propose that this approach may be combined with knowledge about biosynthetic pathways, in this or other microbes, to increase production of lipids and other chemicals. IMPORTANCE This paper reports on experiments to understand how to increase microbial lipid production. Microbial lipids are often cited as one renewable replacement for petroleum-based fuels and chemicals, but strategies to increase the yield of these compounds are needed to achieve this goal. While lipid biosynthesis is often well understood, increasing yields of these compounds to industrially relevant levels is a challenge, especially since genetic, synthetic biology, or engineering approaches are not feasible in many microbes. We show that altering the bacterial cell envelope can be used to increase microbial lipid production. We also find that the utility of some of these alterations can be enhanced by growing cells in bioreactor configurations that can be used industrially. We propose that our findings can inform current and future efforts to increase production of microbial lipids, other fuels, or chemicals that are currently derived from petroleum.Kimberly C. LemmerWeiping ZhangSamantha J. LangerAlice C. DohnalkovaDehong HuRachelle A. LemkeJeff S. PiotrowskiGalya OrrDaniel R. NogueraTimothy J. DonohueAmerican Society for MicrobiologyarticleRhodobacterbioreactorscell envelopefatty acidslipid synthesistwo-component regulatory systemsMicrobiologyQR1-502ENmBio, Vol 8, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rhodobacter bioreactors cell envelope fatty acids lipid synthesis two-component regulatory systems Microbiology QR1-502
spellingShingle	Rhodobacter bioreactors cell envelope fatty acids lipid synthesis two-component regulatory systems Microbiology QR1-502 Kimberly C. Lemmer Weiping Zhang Samantha J. Langer Alice C. Dohnalkova Dehong Hu Rachelle A. Lemke Jeff S. Piotrowski Galya Orr Daniel R. Noguera Timothy J. Donohue Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
description	ABSTRACT Lipids from microbes offer a promising source of renewable alternatives to petroleum-derived compounds. In particular, oleaginous microbes are of interest because they accumulate a large fraction of their biomass as lipids. In this study, we analyzed genetic changes that alter lipid accumulation in Rhodobacter sphaeroides. By screening an R. sphaeroides Tn5 mutant library for insertions that increased fatty acid content, we identified 10 high-lipid (HL) mutants for further characterization. These HL mutants exhibited increased sensitivity to drugs that target the bacterial cell envelope and changes in shape, and some had the ability to secrete lipids, with two HL mutants accumulating ~60% of their total lipids extracellularly. When one of the highest-lipid-secreting strains was grown in a fed-batch bioreactor, its lipid content was comparable to that of oleaginous microbes, with the majority of the lipids secreted into the medium. Based on the properties of these HL mutants, we conclude that alterations of the cell envelope are a previously unreported approach to increase microbial lipid production. We also propose that this approach may be combined with knowledge about biosynthetic pathways, in this or other microbes, to increase production of lipids and other chemicals. IMPORTANCE This paper reports on experiments to understand how to increase microbial lipid production. Microbial lipids are often cited as one renewable replacement for petroleum-based fuels and chemicals, but strategies to increase the yield of these compounds are needed to achieve this goal. While lipid biosynthesis is often well understood, increasing yields of these compounds to industrially relevant levels is a challenge, especially since genetic, synthetic biology, or engineering approaches are not feasible in many microbes. We show that altering the bacterial cell envelope can be used to increase microbial lipid production. We also find that the utility of some of these alterations can be enhanced by growing cells in bioreactor configurations that can be used industrially. We propose that our findings can inform current and future efforts to increase production of microbial lipids, other fuels, or chemicals that are currently derived from petroleum.
format	article
author	Kimberly C. Lemmer Weiping Zhang Samantha J. Langer Alice C. Dohnalkova Dehong Hu Rachelle A. Lemke Jeff S. Piotrowski Galya Orr Daniel R. Noguera Timothy J. Donohue
author_facet	Kimberly C. Lemmer Weiping Zhang Samantha J. Langer Alice C. Dohnalkova Dehong Hu Rachelle A. Lemke Jeff S. Piotrowski Galya Orr Daniel R. Noguera Timothy J. Donohue
author_sort	Kimberly C. Lemmer
title	Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
title_short	Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
title_full	Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
title_fullStr	Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
title_full_unstemmed	Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production
title_sort	mutations that alter the bacterial cell envelope increase lipid production
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/c291e532b948447ebf35d48997c96929
work_keys_str_mv	AT kimberlyclemmer mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT weipingzhang mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT samanthajlanger mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT alicecdohnalkova mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT dehonghu mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT rachellealemke mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT jeffspiotrowski mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT galyaorr mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT danielrnoguera mutationsthatalterthebacterialcellenvelopeincreaselipidproduction AT timothyjdonohue mutationsthatalterthebacterialcellenvelopeincreaselipidproduction
_version_	1718427364476583936

Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production

Ejemplares similares