Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile

Abstract Social insects host a diversity of viruses. We examined New Zealand populations of the globally widely distributed invasive Argentine ant (Linepithema humile) for RNA viruses. We used metatranscriptomic analysis, which identified six potential novel viruses in the Dicistroviridae family. Of...

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Autores principales:	Monica A. M. Gruber, Meghan Cooling, James W. Baty, Kevin Buckley, Anna Friedlander, Oliver Quinn, Jessica F. E. J. Russell, Alexandra Sébastien, Philip J. Lester
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2968244301c4f19a8ba143e2b60c63b
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spelling	oai:doaj.org-article:c2968244301c4f19a8ba143e2b60c63b2021-12-02T16:06:25ZSingle-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile10.1038/s41598-017-03508-z2045-2322https://doaj.org/article/c2968244301c4f19a8ba143e2b60c63b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03508-zhttps://doaj.org/toc/2045-2322Abstract Social insects host a diversity of viruses. We examined New Zealand populations of the globally widely distributed invasive Argentine ant (Linepithema humile) for RNA viruses. We used metatranscriptomic analysis, which identified six potential novel viruses in the Dicistroviridae family. Of these, three contigs were confirmed by Sanger sequencing as Linepithema humile virus-1 (LHUV-1), a novel strain of Kashmir bee virus (KBV) and Black queen cell virus (BQCV), while the others were chimeric or misassembled sequences. We extended the known sequence of LHUV-1 to confirm its placement in the Dicistroviridae and categorised its relationship to closest relatives, which were all viruses infecting Hymenoptera. We examined further for known viruses by mapping our metatranscriptomic sequences to all viral genomes, and confirmed KBV, BQCV, LHUV-1 and Deformed wing virus (DWV) presence using qRT-PCR. Viral replication was confirmed for DWV, KBV and LHUV-1. Viral titers in ants were higher in the presence of honey bee hives. Argentine ants appear to host a range of’ honey bee’ pathogens in addition to a virus currently described only from this invasive ant. The role of these viruses in the population dynamics of the ant remain to be determined, but offer potential targets for biocontrol approaches.Monica A. M. GruberMeghan CoolingJames W. BatyKevin BuckleyAnna FriedlanderOliver QuinnJessica F. E. J. RussellAlexandra SébastienPhilip J. LesterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monica A. M. Gruber Meghan Cooling James W. Baty Kevin Buckley Anna Friedlander Oliver Quinn Jessica F. E. J. Russell Alexandra Sébastien Philip J. Lester Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
description	Abstract Social insects host a diversity of viruses. We examined New Zealand populations of the globally widely distributed invasive Argentine ant (Linepithema humile) for RNA viruses. We used metatranscriptomic analysis, which identified six potential novel viruses in the Dicistroviridae family. Of these, three contigs were confirmed by Sanger sequencing as Linepithema humile virus-1 (LHUV-1), a novel strain of Kashmir bee virus (KBV) and Black queen cell virus (BQCV), while the others were chimeric or misassembled sequences. We extended the known sequence of LHUV-1 to confirm its placement in the Dicistroviridae and categorised its relationship to closest relatives, which were all viruses infecting Hymenoptera. We examined further for known viruses by mapping our metatranscriptomic sequences to all viral genomes, and confirmed KBV, BQCV, LHUV-1 and Deformed wing virus (DWV) presence using qRT-PCR. Viral replication was confirmed for DWV, KBV and LHUV-1. Viral titers in ants were higher in the presence of honey bee hives. Argentine ants appear to host a range of’ honey bee’ pathogens in addition to a virus currently described only from this invasive ant. The role of these viruses in the population dynamics of the ant remain to be determined, but offer potential targets for biocontrol approaches.
format	article
author	Monica A. M. Gruber Meghan Cooling James W. Baty Kevin Buckley Anna Friedlander Oliver Quinn Jessica F. E. J. Russell Alexandra Sébastien Philip J. Lester
author_facet	Monica A. M. Gruber Meghan Cooling James W. Baty Kevin Buckley Anna Friedlander Oliver Quinn Jessica F. E. J. Russell Alexandra Sébastien Philip J. Lester
author_sort	Monica A. M. Gruber
title	Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
title_short	Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
title_full	Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
title_fullStr	Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
title_full_unstemmed	Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile
title_sort	single-stranded rna viruses infecting the invasive argentine ant, linepithema humile
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c2968244301c4f19a8ba143e2b60c63b
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Single-stranded RNA viruses infecting the invasive Argentine ant, Linepithema humile

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