Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines wit...

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Autores principales:	Oliver Xiaoou Dong, Shu Yu, Rashmi Jain, Nan Zhang, Phat Q. Duong, Corinne Butler, Yan Li, Anna Lipzen, Joel A. Martin, Kerrie W. Barry, Jeremy Schmutz, Li Tian, Pamela C. Ronald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c29b6d170b1a4822ba8073858c613766
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spelling	oai:doaj.org-article:c29b6d170b1a4822ba8073858c6137662021-12-02T15:39:13ZMarker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas910.1038/s41467-020-14981-y2041-1723https://doaj.org/article/c29b6d170b1a4822ba8073858c6137662020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14981-yhttps://doaj.org/toc/2041-1723Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines with high carotenoid content.Oliver Xiaoou DongShu YuRashmi JainNan ZhangPhat Q. DuongCorinne ButlerYan LiAnna LipzenJoel A. MartinKerrie W. BarryJeremy SchmutzLi TianPamela C. RonaldNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
description	Existing examples of targeted gene insertion in plants either rely on a selectable marker gene or result in short DNA inserts. Here, the authors use an optimized CRISPR-Cas9 method to insert a 5.2 kb carotenoid biosynthesis cassette into genomic safe harbors in rice, and obtain marker-free lines with high carotenoid content.
format	article
author	Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald
author_facet	Oliver Xiaoou Dong Shu Yu Rashmi Jain Nan Zhang Phat Q. Duong Corinne Butler Yan Li Anna Lipzen Joel A. Martin Kerrie W. Barry Jeremy Schmutz Li Tian Pamela C. Ronald
author_sort	Oliver Xiaoou Dong
title	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_short	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_full	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_fullStr	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_full_unstemmed	Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
title_sort	marker-free carotenoid-enriched rice generated through targeted gene insertion using crispr-cas9
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c29b6d170b1a4822ba8073858c613766
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Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

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