Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions

Abstract Tuberculosis (TB) is still a major worldwide health threat and primarily a lung disease. The innate immune response against Mycobacterium tuberculosis (Mtb) is orchestrated by dendritic cells, macrophages, neutrophils, natural killer cells and apparently mast cells (MCs). MCs are located at...

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Autores principales:	Karen Magdalena Garcia-Rodriguez, Estela Isabel Bini, Armando Gamboa-Domínguez, Clara Inés Espitia-Pinzón, Sara Huerta-Yepez, Silvia Bulfone-Paus, Rogelio Hernández-Pando
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2a45d9a534d4e06a1b51636fd77f89a
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spelling	oai:doaj.org-article:c2a45d9a534d4e06a1b51636fd77f89a2021-12-02T15:45:31ZDifferential mast cell numbers and characteristics in human tuberculosis pulmonary lesions10.1038/s41598-021-89659-62045-2322https://doaj.org/article/c2a45d9a534d4e06a1b51636fd77f89a2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89659-6https://doaj.org/toc/2045-2322Abstract Tuberculosis (TB) is still a major worldwide health threat and primarily a lung disease. The innate immune response against Mycobacterium tuberculosis (Mtb) is orchestrated by dendritic cells, macrophages, neutrophils, natural killer cells and apparently mast cells (MCs). MCs are located at mucosal sites including the lungs and contribute in host-defence against pathogens, but little is known about their role during Mtb infection. This study investigates the location and characteristics of MCs in TB lesions to assess their contribution to TB pathology. To this purpose, number, location and phenotype of MCs was studied in 11 necropsies of pulmonary TB and 3 necropsies of non-TB infected lungs that were used as controls. MCs were localised at pneumonic areas, in the granuloma periphery and particularly abundant in fibrotic tissue. Furthermore, MCs displayed intracellular Mtb and IL-17A and TGF-β immunostaining. These findings were validated by analysing, post-mortem lung tissue microarrays from 44 individuals with pulmonary TB and 25 control subjects. In affected lungs, increased numbers of MCs expressing intracellularly both tryptase and chymase were found at fibrotic sites. Altogether, our data suggest that MCs are recruited at the inflammatory site and that actively produce immune mediators such as proteases and TGF-β that may be contributing to late fibrosis in TB lesions.Karen Magdalena Garcia-RodriguezEstela Isabel BiniArmando Gamboa-DomínguezClara Inés Espitia-PinzónSara Huerta-YepezSilvia Bulfone-PausRogelio Hernández-PandoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen Magdalena Garcia-Rodriguez Estela Isabel Bini Armando Gamboa-Domínguez Clara Inés Espitia-Pinzón Sara Huerta-Yepez Silvia Bulfone-Paus Rogelio Hernández-Pando Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
description	Abstract Tuberculosis (TB) is still a major worldwide health threat and primarily a lung disease. The innate immune response against Mycobacterium tuberculosis (Mtb) is orchestrated by dendritic cells, macrophages, neutrophils, natural killer cells and apparently mast cells (MCs). MCs are located at mucosal sites including the lungs and contribute in host-defence against pathogens, but little is known about their role during Mtb infection. This study investigates the location and characteristics of MCs in TB lesions to assess their contribution to TB pathology. To this purpose, number, location and phenotype of MCs was studied in 11 necropsies of pulmonary TB and 3 necropsies of non-TB infected lungs that were used as controls. MCs were localised at pneumonic areas, in the granuloma periphery and particularly abundant in fibrotic tissue. Furthermore, MCs displayed intracellular Mtb and IL-17A and TGF-β immunostaining. These findings were validated by analysing, post-mortem lung tissue microarrays from 44 individuals with pulmonary TB and 25 control subjects. In affected lungs, increased numbers of MCs expressing intracellularly both tryptase and chymase were found at fibrotic sites. Altogether, our data suggest that MCs are recruited at the inflammatory site and that actively produce immune mediators such as proteases and TGF-β that may be contributing to late fibrosis in TB lesions.
format	article
author	Karen Magdalena Garcia-Rodriguez Estela Isabel Bini Armando Gamboa-Domínguez Clara Inés Espitia-Pinzón Sara Huerta-Yepez Silvia Bulfone-Paus Rogelio Hernández-Pando
author_facet	Karen Magdalena Garcia-Rodriguez Estela Isabel Bini Armando Gamboa-Domínguez Clara Inés Espitia-Pinzón Sara Huerta-Yepez Silvia Bulfone-Paus Rogelio Hernández-Pando
author_sort	Karen Magdalena Garcia-Rodriguez
title	Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
title_short	Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
title_full	Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
title_fullStr	Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
title_full_unstemmed	Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
title_sort	differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2a45d9a534d4e06a1b51636fd77f89a
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Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions

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