A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.

Human induced pluripotent stem cells have the potential to become an unlimited cell source for cell replacement therapy. The realization of this potential, however, depends on the availability of culture methods that are robust, scalable, and use chemically defined materials. Despite significant adv...

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Autores principales:	Sha Jin, Huantong Yao, Jennifer L Weber, Zara K Melkoumian, Kaiming Ye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2a4e7f779c54a3ba04d55645af21166
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spelling	oai:doaj.org-article:c2a4e7f779c54a3ba04d55645af211662021-11-18T08:06:39ZA synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.1932-620310.1371/journal.pone.0050880https://doaj.org/article/c2a4e7f779c54a3ba04d55645af211662012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226418/?tool=EBIhttps://doaj.org/toc/1932-6203Human induced pluripotent stem cells have the potential to become an unlimited cell source for cell replacement therapy. The realization of this potential, however, depends on the availability of culture methods that are robust, scalable, and use chemically defined materials. Despite significant advances in hiPSC technologies, the expansion of hiPSCs relies upon the use of animal-derived extracellular matrix extracts, such as Matrigel, which raises safety concerns over the use of these products. In this work, we investigated the feasibility of expanding and differentiating hiPSCs on a chemically defined, xeno-free synthetic peptide substrate, i.e. Corning Synthemax(®) Surface. We demonstrated that the Synthemax Surface supports the attachment, spreading, and proliferation of hiPSCs, as well as hiPSCs' lineage-specific differentiation. hiPSCs colonies grown on Synthemax Surfaces exhibit less spread and more compact morphology compared to cells grown on Matrigel™. The cytoskeleton characterization of hiPSCs grown on the Synthemax Surface revealed formation of denser actin filaments in the cell-cell interface. The down-regulation of vinculin and up-regulation of zyxin expression were also observed in hiPSCs grown on the Synthemax Surface. Further examination of cell-ECM interaction revealed that hiPSCs grown on the Synthemax Surface primarily utilize α(v)β(5) integrins to mediate attachment to the substrate, whereas multiple integrins are involved in cell attachment to Matrigel. Finally, hiPSCs can be maintained undifferentiated on the Synthemax Surface for more than ten passages. These studies provide a novel approach for expansion of hiPSCs using synthetic peptide engineered surface as a substrate to avoid a potential risk of contamination and lot-to-lot variability with animal derived materials.Sha JinHuantong YaoJennifer L WeberZara K MelkoumianKaiming YePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50880 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sha Jin Huantong Yao Jennifer L Weber Zara K Melkoumian Kaiming Ye A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
description	Human induced pluripotent stem cells have the potential to become an unlimited cell source for cell replacement therapy. The realization of this potential, however, depends on the availability of culture methods that are robust, scalable, and use chemically defined materials. Despite significant advances in hiPSC technologies, the expansion of hiPSCs relies upon the use of animal-derived extracellular matrix extracts, such as Matrigel, which raises safety concerns over the use of these products. In this work, we investigated the feasibility of expanding and differentiating hiPSCs on a chemically defined, xeno-free synthetic peptide substrate, i.e. Corning Synthemax(®) Surface. We demonstrated that the Synthemax Surface supports the attachment, spreading, and proliferation of hiPSCs, as well as hiPSCs' lineage-specific differentiation. hiPSCs colonies grown on Synthemax Surfaces exhibit less spread and more compact morphology compared to cells grown on Matrigel™. The cytoskeleton characterization of hiPSCs grown on the Synthemax Surface revealed formation of denser actin filaments in the cell-cell interface. The down-regulation of vinculin and up-regulation of zyxin expression were also observed in hiPSCs grown on the Synthemax Surface. Further examination of cell-ECM interaction revealed that hiPSCs grown on the Synthemax Surface primarily utilize α(v)β(5) integrins to mediate attachment to the substrate, whereas multiple integrins are involved in cell attachment to Matrigel. Finally, hiPSCs can be maintained undifferentiated on the Synthemax Surface for more than ten passages. These studies provide a novel approach for expansion of hiPSCs using synthetic peptide engineered surface as a substrate to avoid a potential risk of contamination and lot-to-lot variability with animal derived materials.
format	article
author	Sha Jin Huantong Yao Jennifer L Weber Zara K Melkoumian Kaiming Ye
author_facet	Sha Jin Huantong Yao Jennifer L Weber Zara K Melkoumian Kaiming Ye
author_sort	Sha Jin
title	A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
title_short	A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
title_full	A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
title_fullStr	A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
title_full_unstemmed	A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
title_sort	synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c2a4e7f779c54a3ba04d55645af21166
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A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells.

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