Optimized CRISPR-Cas9 Genome Editing for <italic toggle="yes">Leishmania</italic> and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms
ABSTRACT CRISPR-Cas9-mediated genome editing has recently been adapted for Leishmania spp. parasites, the causative agents of human leishmaniasis. We have optimized this genome-editing tool by selecting for cells with CRISPR-Cas9 activity through cotargeting the miltefosine transporter gene; mutatio...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
American Society for Microbiology
2017
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| Subjects: | |
| Online Access: | https://doaj.org/article/c2a6be188d9c4d9ca3e338b03e97bfb0 |
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