Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.

MicroRNAs (miRNAs) are short, non-coding RNAs, which post-transcriptionally regulate gene expression and are proposed to play a key role in the regulation of innate and adaptive immunity. Here, we report a next generation sequencing (NGS) approach profiling the expression of miRNAs in primary bovine...

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Autores principales:	Nathan Lawless, Amir B K Foroushani, Matthew S McCabe, Cliona O'Farrelly, David J Lynn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2b8363d9ad4456cb795ff32942983e2
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spelling	oai:doaj.org-article:c2b8363d9ad4456cb795ff32942983e22021-11-18T07:54:57ZNext generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.1932-620310.1371/journal.pone.0057543https://doaj.org/article/c2b8363d9ad4456cb795ff32942983e22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23472090/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are short, non-coding RNAs, which post-transcriptionally regulate gene expression and are proposed to play a key role in the regulation of innate and adaptive immunity. Here, we report a next generation sequencing (NGS) approach profiling the expression of miRNAs in primary bovine mammary epithelial cells (BMEs) at 1, 2, 4 and 6 hours post-infection with Streptococcus uberis, a causative agent of bovine mastitis. Analysing over 450 million sequencing reads, we found that 20% of the approximately 1,300 currently known bovine miRNAs are expressed in unchallenged BMEs. We also identified the expression of more than 20 potentially novel bovine miRNAs. There is, however, a significant dynamic range in the expression of known miRNAs. The top 10 highly expressed miRNAs account for >80% of all aligned reads, with the remaining miRNAs showing much lower expression. Twenty-one miRNAs were identified as significantly differentially expressed post-infection with S. uberis. Several of these miRNAs have characterised roles in the immune systems of other species. This miRNA response to the Gram-positive S. uberis is markedly different, however, to lipopolysaccharide (LPS) induced miRNA expression. Of 145 miRNAs identified in the literature as being LPS responsive, only 9 were also differentially expressed in response to S. uberis. Computational analysis has also revealed that the predicted target genes of miRNAs, which are down-regulated in BMEs following S. uberis infection, are statistically enriched for roles in innate immunity. This suggests that miRNAs, which potentially act as central regulators of gene expression responses to a Gram-positive bacterial infection, may significantly regulate the sentinel capacity of mammary epithelial cells to mobilise the innate immune system.Nathan LawlessAmir B K ForoushaniMatthew S McCabeCliona O'FarrellyDavid J LynnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57543 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathan Lawless Amir B K Foroushani Matthew S McCabe Cliona O'Farrelly David J Lynn Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
description	MicroRNAs (miRNAs) are short, non-coding RNAs, which post-transcriptionally regulate gene expression and are proposed to play a key role in the regulation of innate and adaptive immunity. Here, we report a next generation sequencing (NGS) approach profiling the expression of miRNAs in primary bovine mammary epithelial cells (BMEs) at 1, 2, 4 and 6 hours post-infection with Streptococcus uberis, a causative agent of bovine mastitis. Analysing over 450 million sequencing reads, we found that 20% of the approximately 1,300 currently known bovine miRNAs are expressed in unchallenged BMEs. We also identified the expression of more than 20 potentially novel bovine miRNAs. There is, however, a significant dynamic range in the expression of known miRNAs. The top 10 highly expressed miRNAs account for >80% of all aligned reads, with the remaining miRNAs showing much lower expression. Twenty-one miRNAs were identified as significantly differentially expressed post-infection with S. uberis. Several of these miRNAs have characterised roles in the immune systems of other species. This miRNA response to the Gram-positive S. uberis is markedly different, however, to lipopolysaccharide (LPS) induced miRNA expression. Of 145 miRNAs identified in the literature as being LPS responsive, only 9 were also differentially expressed in response to S. uberis. Computational analysis has also revealed that the predicted target genes of miRNAs, which are down-regulated in BMEs following S. uberis infection, are statistically enriched for roles in innate immunity. This suggests that miRNAs, which potentially act as central regulators of gene expression responses to a Gram-positive bacterial infection, may significantly regulate the sentinel capacity of mammary epithelial cells to mobilise the innate immune system.
format	article
author	Nathan Lawless Amir B K Foroushani Matthew S McCabe Cliona O'Farrelly David J Lynn
author_facet	Nathan Lawless Amir B K Foroushani Matthew S McCabe Cliona O'Farrelly David J Lynn
author_sort	Nathan Lawless
title	Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
title_short	Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
title_full	Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
title_fullStr	Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
title_full_unstemmed	Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.
title_sort	next generation sequencing reveals the expression of a unique mirna profile in response to a gram-positive bacterial infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c2b8363d9ad4456cb795ff32942983e2
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Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection.

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