A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition

Abstract Embryonic stem cells (ESCs) and epiblast-like cells (EpiLCs) recapitulate in vitro the epiblast first cell lineage decision, allowing characterization of the molecular mechanisms underlying pluripotent state transition. Here, we performed a comprehensive and comparative analysis of total gl...

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Autores principales:	Federico Pecori, Ikuko Yokota, Hisatoshi Hanamatsu, Taichi Miura, Chika Ogura, Hayato Ota, Jun-ichi Furukawa, Shinya Oki, Kazuo Yamamoto, Osamu Yoshie, Shoko Nishihara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2bda6e3df6349d5b749d60c512ff8ee
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spelling	oai:doaj.org-article:c2bda6e3df6349d5b749d60c512ff8ee2021-12-02T14:12:47ZA defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition10.1038/s41598-020-79666-42045-2322https://doaj.org/article/c2bda6e3df6349d5b749d60c512ff8ee2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79666-4https://doaj.org/toc/2045-2322Abstract Embryonic stem cells (ESCs) and epiblast-like cells (EpiLCs) recapitulate in vitro the epiblast first cell lineage decision, allowing characterization of the molecular mechanisms underlying pluripotent state transition. Here, we performed a comprehensive and comparative analysis of total glycomes of mouse ESCs and EpiLCs, revealing that overall glycosylation undergoes dramatic changes from early stages of development. Remarkably, we showed for the first time the presence of a developmentally regulated network orchestrating glycosylation changes and identified polycomb repressive complex 2 (PRC2) as a key component involved in this process. Collectively, our findings provide novel insights into the naïve-to-primed pluripotent state transition and advance the understanding of glycosylation complex regulation during early mouse embryonic development.Federico PecoriIkuko YokotaHisatoshi HanamatsuTaichi MiuraChika OguraHayato OtaJun-ichi FurukawaShinya OkiKazuo YamamotoOsamu YoshieShoko NishiharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Federico Pecori Ikuko Yokota Hisatoshi Hanamatsu Taichi Miura Chika Ogura Hayato Ota Jun-ichi Furukawa Shinya Oki Kazuo Yamamoto Osamu Yoshie Shoko Nishihara A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
description	Abstract Embryonic stem cells (ESCs) and epiblast-like cells (EpiLCs) recapitulate in vitro the epiblast first cell lineage decision, allowing characterization of the molecular mechanisms underlying pluripotent state transition. Here, we performed a comprehensive and comparative analysis of total glycomes of mouse ESCs and EpiLCs, revealing that overall glycosylation undergoes dramatic changes from early stages of development. Remarkably, we showed for the first time the presence of a developmentally regulated network orchestrating glycosylation changes and identified polycomb repressive complex 2 (PRC2) as a key component involved in this process. Collectively, our findings provide novel insights into the naïve-to-primed pluripotent state transition and advance the understanding of glycosylation complex regulation during early mouse embryonic development.
format	article
author	Federico Pecori Ikuko Yokota Hisatoshi Hanamatsu Taichi Miura Chika Ogura Hayato Ota Jun-ichi Furukawa Shinya Oki Kazuo Yamamoto Osamu Yoshie Shoko Nishihara
author_facet	Federico Pecori Ikuko Yokota Hisatoshi Hanamatsu Taichi Miura Chika Ogura Hayato Ota Jun-ichi Furukawa Shinya Oki Kazuo Yamamoto Osamu Yoshie Shoko Nishihara
author_sort	Federico Pecori
title	A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
title_short	A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
title_full	A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
title_fullStr	A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
title_full_unstemmed	A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
title_sort	defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2bda6e3df6349d5b749d60c512ff8ee
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A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition

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