Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand

Abstract The rotating-crystal magneto-optical detection (RMOD) method has been developed for the rapid and quantitative diagnosis of malaria and tested systematically on various malaria infection models. Very recently, an extended field trial in a high-transmission region of Papua New Guinea demonst...

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Autores principales:	Ágnes Orbán, Rhea J. Longley, Piyarat Sripoorote, Nongnuj Maneechai, Wang Nguitragool, Ádám Butykai, Ivo Mueller, Jetsumon Sattabongkot, Stephan Karl, István Kézsmárki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2bf180ef8d84e03afcb4a88bb94edd6
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spelling	oai:doaj.org-article:c2bf180ef8d84e03afcb4a88bb94edd62021-12-02T18:02:22ZSensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand10.1038/s41598-021-97532-92045-2322https://doaj.org/article/c2bf180ef8d84e03afcb4a88bb94edd62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97532-9https://doaj.org/toc/2045-2322Abstract The rotating-crystal magneto-optical detection (RMOD) method has been developed for the rapid and quantitative diagnosis of malaria and tested systematically on various malaria infection models. Very recently, an extended field trial in a high-transmission region of Papua New Guinea demonstrated its great potential for detecting malaria infections, in particular Plasmodium vivax. In the present small-scale field test, carried out in a low-transmission area of Thailand, RMOD confirmed malaria in all samples found to be infected with Plasmodium vivax by microscopy, our reference method. Moreover, the magneto-optical signal for this sample set was typically 1–3 orders of magnitude higher than the cut-off value of RMOD determined on uninfected samples. Based on the serial dilution of the original patient samples, we expect that the method can detect Plasmodium vivax malaria in blood samples with parasite densities as low as $$\sim$$ ∼ 5–10 parasites per microliter, a limit around the pyrogenic threshold of the infection. In addition, by investigating the correlation between the magnitude of the magneto-optical signal, the parasite density and the erythrocytic stage distribution, we estimate the relative hemozoin production rates of the ring and the trophozoite stages of in vivo Plasmodium vivax infections.Ágnes OrbánRhea J. LongleyPiyarat SripooroteNongnuj ManeechaiWang NguitragoolÁdám ButykaiIvo MuellerJetsumon SattabongkotStephan KarlIstván KézsmárkiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ágnes Orbán Rhea J. Longley Piyarat Sripoorote Nongnuj Maneechai Wang Nguitragool Ádám Butykai Ivo Mueller Jetsumon Sattabongkot Stephan Karl István Kézsmárki Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
description	Abstract The rotating-crystal magneto-optical detection (RMOD) method has been developed for the rapid and quantitative diagnosis of malaria and tested systematically on various malaria infection models. Very recently, an extended field trial in a high-transmission region of Papua New Guinea demonstrated its great potential for detecting malaria infections, in particular Plasmodium vivax. In the present small-scale field test, carried out in a low-transmission area of Thailand, RMOD confirmed malaria in all samples found to be infected with Plasmodium vivax by microscopy, our reference method. Moreover, the magneto-optical signal for this sample set was typically 1–3 orders of magnitude higher than the cut-off value of RMOD determined on uninfected samples. Based on the serial dilution of the original patient samples, we expect that the method can detect Plasmodium vivax malaria in blood samples with parasite densities as low as $$\sim$$ ∼ 5–10 parasites per microliter, a limit around the pyrogenic threshold of the infection. In addition, by investigating the correlation between the magnitude of the magneto-optical signal, the parasite density and the erythrocytic stage distribution, we estimate the relative hemozoin production rates of the ring and the trophozoite stages of in vivo Plasmodium vivax infections.
format	article
author	Ágnes Orbán Rhea J. Longley Piyarat Sripoorote Nongnuj Maneechai Wang Nguitragool Ádám Butykai Ivo Mueller Jetsumon Sattabongkot Stephan Karl István Kézsmárki
author_facet	Ágnes Orbán Rhea J. Longley Piyarat Sripoorote Nongnuj Maneechai Wang Nguitragool Ádám Butykai Ivo Mueller Jetsumon Sattabongkot Stephan Karl István Kézsmárki
author_sort	Ágnes Orbán
title	Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
title_short	Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
title_full	Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
title_fullStr	Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
title_full_unstemmed	Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand
title_sort	sensitive detection of plasmodium vivax malaria by the rotating-crystal magneto-optical method in thailand
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2bf180ef8d84e03afcb4a88bb94edd6
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Sensitive detection of Plasmodium vivax malaria by the rotating-crystal magneto-optical method in Thailand

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