Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms
ABSTRACT Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c2c3ee7022994291bb1fe6c63a28e695 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c2c3ee7022994291bb1fe6c63a28e695 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c2c3ee7022994291bb1fe6c63a28e6952021-11-15T15:22:04ZArginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms10.1128/mSphere.00295-172379-5042https://doaj.org/article/c2c3ee7022994291bb1fe6c63a28e6952017-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00295-17https://doaj.org/toc/2379-5042ABSTRACT Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under controlled conditions. Plaque microcosms were established in constant-depth film fermentors (CDFFs) using salivary inocula and fed continuously with artificial saliva. To simulate resting and cariogenic states, the CDFFs were supplemented with sterile water or 5% sucrose, respectively. Plaques were then dosed twice daily with a dentifrice with 1.5% arginine arginine added (DA) or without arginine (DN). This regimen continued for over 3 weeks, after which fermentors were maintained without dosing. Microcosms were analyzed by differential viable counting, with a pH microelectrode, and by eubacterial DNA profiling. Sucrose dosing was associated with significantly (P < 0.001) decreased pH, significantly (P < 0.05) increased counts of total aerobes, Gram-negative anaerobes, aciduric species, acidogenic species, arginine utilizing species, bifidobacteria, lactobacilli and streptococci, and significant (P < 0.05) changes in DNA profiles. Plaques dosed with DA had a significantly (P < 0.001) higher pH than those dosed with DN, with or without sucrose supplementation. Dosing with DA but not DN significantly (P < 0.05) decreased counts of all functional bacterial groups apart from the total anaerobes in cariogenic plaques, and in resting plaques, dosing with DA significantly (P < 0.05) decreased counts of streptococci, lactobacilli, bifidobacteria, and acidogenic bacteria. In summary, sustained exposure of oral microcosms to arginine in formulation significantly increased plaque pH and significantly reduced the viability of cariogenic bacterial species. IMPORTANCE Arginine is used in dental health formulations to help prevent dental cavities. This study assessed the effects of the long-term dosing of laboratory dental plaques with an arginine dentifrice. Data indicate that the addition of arginine dentifrice during sucrose challenge significantly increased plaque pH, thus potentially mitigating cariogenesis. Counts of several functional groups of bacteria associated with tooth decay were significantly decreased in the laboratory plaques during exposure to the arginine dentifrice.Ruth G. LedderHitesh MistryPrem K. SreenivasanGavin HumphreysAndrew J. McBainAmerican Society for Microbiologyarticleargininedental biofilm microcosmdental plaqueMicrobiologyQR1-502ENmSphere, Vol 2, Iss 4 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
arginine dental biofilm microcosm dental plaque Microbiology QR1-502 |
| spellingShingle |
arginine dental biofilm microcosm dental plaque Microbiology QR1-502 Ruth G. Ledder Hitesh Mistry Prem K. Sreenivasan Gavin Humphreys Andrew J. McBain Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| description |
ABSTRACT Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under controlled conditions. Plaque microcosms were established in constant-depth film fermentors (CDFFs) using salivary inocula and fed continuously with artificial saliva. To simulate resting and cariogenic states, the CDFFs were supplemented with sterile water or 5% sucrose, respectively. Plaques were then dosed twice daily with a dentifrice with 1.5% arginine arginine added (DA) or without arginine (DN). This regimen continued for over 3 weeks, after which fermentors were maintained without dosing. Microcosms were analyzed by differential viable counting, with a pH microelectrode, and by eubacterial DNA profiling. Sucrose dosing was associated with significantly (P < 0.001) decreased pH, significantly (P < 0.05) increased counts of total aerobes, Gram-negative anaerobes, aciduric species, acidogenic species, arginine utilizing species, bifidobacteria, lactobacilli and streptococci, and significant (P < 0.05) changes in DNA profiles. Plaques dosed with DA had a significantly (P < 0.001) higher pH than those dosed with DN, with or without sucrose supplementation. Dosing with DA but not DN significantly (P < 0.05) decreased counts of all functional bacterial groups apart from the total anaerobes in cariogenic plaques, and in resting plaques, dosing with DA significantly (P < 0.05) decreased counts of streptococci, lactobacilli, bifidobacteria, and acidogenic bacteria. In summary, sustained exposure of oral microcosms to arginine in formulation significantly increased plaque pH and significantly reduced the viability of cariogenic bacterial species. IMPORTANCE Arginine is used in dental health formulations to help prevent dental cavities. This study assessed the effects of the long-term dosing of laboratory dental plaques with an arginine dentifrice. Data indicate that the addition of arginine dentifrice during sucrose challenge significantly increased plaque pH, thus potentially mitigating cariogenesis. Counts of several functional groups of bacteria associated with tooth decay were significantly decreased in the laboratory plaques during exposure to the arginine dentifrice. |
| format |
article |
| author |
Ruth G. Ledder Hitesh Mistry Prem K. Sreenivasan Gavin Humphreys Andrew J. McBain |
| author_facet |
Ruth G. Ledder Hitesh Mistry Prem K. Sreenivasan Gavin Humphreys Andrew J. McBain |
| author_sort |
Ruth G. Ledder |
| title |
Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| title_short |
Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| title_full |
Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| title_fullStr |
Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| title_full_unstemmed |
Arginine Exposure Decreases Acidogenesis in Long-Term Oral Biofilm Microcosms |
| title_sort |
arginine exposure decreases acidogenesis in long-term oral biofilm microcosms |
| publisher |
American Society for Microbiology |
| publishDate |
2017 |
| url |
https://doaj.org/article/c2c3ee7022994291bb1fe6c63a28e695 |
| work_keys_str_mv |
AT ruthgledder arginineexposuredecreasesacidogenesisinlongtermoralbiofilmmicrocosms AT hiteshmistry arginineexposuredecreasesacidogenesisinlongtermoralbiofilmmicrocosms AT premksreenivasan arginineexposuredecreasesacidogenesisinlongtermoralbiofilmmicrocosms AT gavinhumphreys arginineexposuredecreasesacidogenesisinlongtermoralbiofilmmicrocosms AT andrewjmcbain arginineexposuredecreasesacidogenesisinlongtermoralbiofilmmicrocosms |
| _version_ |
1718428095555305472 |