Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.

Brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the VirB type IV secretion system and the flagellar genes. However, the basis of quorum sensing, namely the production of autoinducers in Brucella has been questioned. Here...

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Autores principales:	Matthieu Terwagne, Aurélie Mirabella, Julien Lemaire, Chantal Deschamps, Xavier De Bolle, Jean-Jacques Letesson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2c59d003b5d4641afbf9d8ce1604261
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spelling	oai:doaj.org-article:c2c59d003b5d4641afbf9d8ce16042612021-11-18T08:42:25ZQuorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.1932-620310.1371/journal.pone.0082514https://doaj.org/article/c2c59d003b5d4641afbf9d8ce16042612013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349302/?tool=EBIhttps://doaj.org/toc/1932-6203Brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the VirB type IV secretion system and the flagellar genes. However, the basis of quorum sensing, namely the production of autoinducers in Brucella has been questioned. Here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-chain N-acyl-homoserine lactones (AHL) activity at single bacterium level in Brucella melitensis. These data are consistent with an intrinsic production of AHL by B. melitensis in low concentration both during in vitro growth and macrophage infection. Moreover, we identified a protein, named AibP, which is homologous to the AHL-acylases of various bacterial species. In vitro and during infection, expression of aibP coincided with a decrease in endogenous AHL activity within B. melitensis, suggesting that AibP could efficiently impair AHL accumulation. Furthermore, we showed that deletion of aibP in B. melitensis resulted in enhanced virB genes expression and VirB8 production as well as in a reduced flagellar genes expression and production of FlgE (hook protein) and FliC (flagellin) in vitro. Altogether, these results suggest that AHL-dependent quorum sensing and AHL-quorum quenching coexist in Brucella, at least to regulate its virulence.Matthieu TerwagneAurélie MirabellaJulien LemaireChantal DeschampsXavier De BolleJean-Jacques LetessonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82514 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthieu Terwagne Aurélie Mirabella Julien Lemaire Chantal Deschamps Xavier De Bolle Jean-Jacques Letesson Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
description	Brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the VirB type IV secretion system and the flagellar genes. However, the basis of quorum sensing, namely the production of autoinducers in Brucella has been questioned. Here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-chain N-acyl-homoserine lactones (AHL) activity at single bacterium level in Brucella melitensis. These data are consistent with an intrinsic production of AHL by B. melitensis in low concentration both during in vitro growth and macrophage infection. Moreover, we identified a protein, named AibP, which is homologous to the AHL-acylases of various bacterial species. In vitro and during infection, expression of aibP coincided with a decrease in endogenous AHL activity within B. melitensis, suggesting that AibP could efficiently impair AHL accumulation. Furthermore, we showed that deletion of aibP in B. melitensis resulted in enhanced virB genes expression and VirB8 production as well as in a reduced flagellar genes expression and production of FlgE (hook protein) and FliC (flagellin) in vitro. Altogether, these results suggest that AHL-dependent quorum sensing and AHL-quorum quenching coexist in Brucella, at least to regulate its virulence.
format	article
author	Matthieu Terwagne Aurélie Mirabella Julien Lemaire Chantal Deschamps Xavier De Bolle Jean-Jacques Letesson
author_facet	Matthieu Terwagne Aurélie Mirabella Julien Lemaire Chantal Deschamps Xavier De Bolle Jean-Jacques Letesson
author_sort	Matthieu Terwagne
title	Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
title_short	Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
title_full	Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
title_fullStr	Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
title_full_unstemmed	Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.
title_sort	quorum sensing and self-quorum quenching in the intracellular pathogen brucellamelitensis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c2c59d003b5d4641afbf9d8ce1604261
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Quorum sensing and self-quorum quenching in the intracellular pathogen Brucellamelitensis.

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