Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers

Abstract Although the type 4 secretion system of the integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori, its clinical association with the cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. pylori samples (46 duode...

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Autores principales:	Bui Hoang Phuc, Vo Phuoc Tuan, Ho Dang Quy Dung, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ngo Phuong Minh Thuan, Vu Van Khien, Tran Thi Huyen Trang, Junko Akada, Takeshi Matsumoto, Yoshio Yamaoka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2cdfc20d26b4048a0a636e5421730e3
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spelling	oai:doaj.org-article:c2cdfc20d26b4048a0a636e5421730e32021-12-02T13:19:29ZHelicobacter pylori type 4 secretion systems as gastroduodenal disease markers10.1038/s41598-021-83862-12045-2322https://doaj.org/article/c2cdfc20d26b4048a0a636e5421730e32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83862-1https://doaj.org/toc/2045-2322Abstract Although the type 4 secretion system of the integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori, its clinical association with the cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. pylori samples (46 duodenal ulcer (DU), 51 non-cardia gastric cancer (NCGC), 39 chronic gastritis (CG)) were fully sequenced using next-generation sequencing and assembled into contigs. tfs3, tfs4, and cagPAI genes were compared with the public database. Most (94%) H. pylori strains possessed a complete cagPAI, which was the greatest risk factor for clinical outcomes, while the prevalences of tfs3 and tfs4 were 45% and 77%, respectively. Complete tfs3 and tfs4 were found in 18.3% and 17.6% of strains, respectively. The prevalence of H. pylori strains with complete tfs3 ICE in DU patients was significantly higher than that in NCGC patients (30.4% vs 11.7%, P < 0.05). In addition, the prevalence of strains with complete tfs3 ICE and cagPAI was significantly higher in DU patients than that in NCGC (28.4% vs 9.8%, P = 0.038) and CG patients (28.2% vs 7.7%, P = 0.024). cagPAI and complete tfs3 increased the risk of DU compared to NCGC (OR = 3.56, 95%CI: 1.1–14.1, P = 0.038) and CG (OR = 4.64, 95%CI: 1.1–27.6, P = 0.024). A complete cluster of tfs3 ICE was associated with gastroduodenal diseases in Vietnam. However, there was a low prevalence of the dupA/complete dupA cluster (15.4%) in the Vietnam strains. The prevalence of cagPAI in Vietnam strains was significantly higher than in US (P = 0.01) and Indonesia (P < 0.0001); the prevalence of the dupA cluster was also higher in the Vietnam strains than in the Indonesian strains (P < 0.05). In addition, the prevalence of ctkA, an accessory gene of tfs3, was significantly different between Vietnam and US strains (28% vs 2%, P = 0.0002). In summary, the acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.Bui Hoang PhucVo Phuoc TuanHo Dang Quy DungTran Thanh BinhPham Huu TungTran Dinh TriNgo Phuong Minh ThuanVu Van KhienTran Thi Huyen TrangJunko AkadaTakeshi MatsumotoYoshio YamaokaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bui Hoang Phuc Vo Phuoc Tuan Ho Dang Quy Dung Tran Thanh Binh Pham Huu Tung Tran Dinh Tri Ngo Phuong Minh Thuan Vu Van Khien Tran Thi Huyen Trang Junko Akada Takeshi Matsumoto Yoshio Yamaoka Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
description	Abstract Although the type 4 secretion system of the integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori, its clinical association with the cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. pylori samples (46 duodenal ulcer (DU), 51 non-cardia gastric cancer (NCGC), 39 chronic gastritis (CG)) were fully sequenced using next-generation sequencing and assembled into contigs. tfs3, tfs4, and cagPAI genes were compared with the public database. Most (94%) H. pylori strains possessed a complete cagPAI, which was the greatest risk factor for clinical outcomes, while the prevalences of tfs3 and tfs4 were 45% and 77%, respectively. Complete tfs3 and tfs4 were found in 18.3% and 17.6% of strains, respectively. The prevalence of H. pylori strains with complete tfs3 ICE in DU patients was significantly higher than that in NCGC patients (30.4% vs 11.7%, P < 0.05). In addition, the prevalence of strains with complete tfs3 ICE and cagPAI was significantly higher in DU patients than that in NCGC (28.4% vs 9.8%, P = 0.038) and CG patients (28.2% vs 7.7%, P = 0.024). cagPAI and complete tfs3 increased the risk of DU compared to NCGC (OR = 3.56, 95%CI: 1.1–14.1, P = 0.038) and CG (OR = 4.64, 95%CI: 1.1–27.6, P = 0.024). A complete cluster of tfs3 ICE was associated with gastroduodenal diseases in Vietnam. However, there was a low prevalence of the dupA/complete dupA cluster (15.4%) in the Vietnam strains. The prevalence of cagPAI in Vietnam strains was significantly higher than in US (P = 0.01) and Indonesia (P < 0.0001); the prevalence of the dupA cluster was also higher in the Vietnam strains than in the Indonesian strains (P < 0.05). In addition, the prevalence of ctkA, an accessory gene of tfs3, was significantly different between Vietnam and US strains (28% vs 2%, P = 0.0002). In summary, the acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.
format	article
author	Bui Hoang Phuc Vo Phuoc Tuan Ho Dang Quy Dung Tran Thanh Binh Pham Huu Tung Tran Dinh Tri Ngo Phuong Minh Thuan Vu Van Khien Tran Thi Huyen Trang Junko Akada Takeshi Matsumoto Yoshio Yamaoka
author_facet	Bui Hoang Phuc Vo Phuoc Tuan Ho Dang Quy Dung Tran Thanh Binh Pham Huu Tung Tran Dinh Tri Ngo Phuong Minh Thuan Vu Van Khien Tran Thi Huyen Trang Junko Akada Takeshi Matsumoto Yoshio Yamaoka
author_sort	Bui Hoang Phuc
title	Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
title_short	Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
title_full	Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
title_fullStr	Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
title_full_unstemmed	Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
title_sort	helicobacter pylori type 4 secretion systems as gastroduodenal disease markers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2cdfc20d26b4048a0a636e5421730e3
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Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers

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