Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering

Biwei Deng,1,2 Angela Bruzzaniti,3 Gary J Cheng1,2 1School of Industrial Engineering, Purdue University, West Lafayette, IN 47907, USA; 2Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA; 3Department of Biomedical and Applied Sciences, Indiana University School of Dentis...

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Autores principales:	Deng B, Bruzzaniti A, Cheng GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	metal implants bone regeneration osteoinductive behavior pulsed laser coating biocompatibility differentiation alkaline phosphatase Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c2d470a2e5ad4fa29c36814625dc7974
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spelling	oai:doaj.org-article:c2d470a2e5ad4fa29c36814625dc79742021-12-02T09:00:22ZEnhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering1178-2013https://doaj.org/article/c2d470a2e5ad4fa29c36814625dc79742018-11-01T00:00:00Zhttps://www.dovepress.com/enhancement-of-osteoblast-activity-on-nanostructured-nitihydroxyapatit-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Biwei Deng,1,2 Angela Bruzzaniti,3 Gary J Cheng1,2 1School of Industrial Engineering, Purdue University, West Lafayette, IN 47907, USA; 2Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA; 3Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN 46202, USA Background: The osteoinductive behaviors of nitinol (NiTi)-based metal implants for bone regeneration are largely dependent on their surface composition and topology. Continuous-mode laser sintering often results in complete melting of the materials and aggregation of particles, which lack control of heat transfer, as well as microstructural changes during sintering of the nanocomposite materials.Methods: In the current study, in situ direct laser deposition was used to additively manufacture three-dimensional NiTi structures from Ni and Ti powders. The mechanical property of NiTi has been shown to be similar to bone. Nanosecond pulsed laser sintering process was then utilized to generate a nanoporous composite surface with NiTi alloy and hydroxyapatite (HA) by ultrafast laser heating and cooling of Ni, Ti, and HA nanoparticles mixtures precoated on the 3D NiTi substrates; HA was added in order to improve the biocompatibility of the alloy. We then studied the underlying mechanism in the formation of NiTi/HA nanocomposite, and the synergistic effect of the sintered HA component and the nanoporous topology of the composite coating. In addition, we examined the activity of bone-forming osteoblasts on the NiTi/HA surfaces. For this, osteoblast cell morphology and various biomarkers were examined to evaluate cellular activity and function.Results: We found that the nanoscale porosity delivered by nanosecond pulsed laser sintering and the HA component positively contributed to osteoblast differentiation, as indicated by an increase in the expression of collagen and alkaline phosphatase, both of which are necessary for osteoblast mineralization. In addition, we observed topological complexities which appeared to boost the activity of osteoblasts, including an increase in actin cytoskeletal structures and adhesion structures.Conclusion: These findings demonstrate that the pulsed laser sintering method is an effective tool to generate biocompatible coatings in complex alloy-composite material systems with desired composition and topology. Our findings also provide a better understanding of the osteoinductive behavior of the sintered nanocomposite coatings for use in orthopedic and bone regeneration applications. Keywords: metal implants, bone regeneration, osteoinductive behavior, pulsed laser coating, biocompatibility, differentiation, alkaline phosphataseDeng BBruzzaniti ACheng GJDove Medical Pressarticlemetal implantsbone regenerationosteoinductive behaviorpulsed laser coatingbiocompatibilitydifferentiationalkaline phosphataseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 8217-8230 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	metal implants bone regeneration osteoinductive behavior pulsed laser coating biocompatibility differentiation alkaline phosphatase Medicine (General) R5-920
spellingShingle	metal implants bone regeneration osteoinductive behavior pulsed laser coating biocompatibility differentiation alkaline phosphatase Medicine (General) R5-920 Deng B Bruzzaniti A Cheng GJ Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
description	Biwei Deng,1,2 Angela Bruzzaniti,3 Gary J Cheng1,2 1School of Industrial Engineering, Purdue University, West Lafayette, IN 47907, USA; 2Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA; 3Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN 46202, USA Background: The osteoinductive behaviors of nitinol (NiTi)-based metal implants for bone regeneration are largely dependent on their surface composition and topology. Continuous-mode laser sintering often results in complete melting of the materials and aggregation of particles, which lack control of heat transfer, as well as microstructural changes during sintering of the nanocomposite materials.Methods: In the current study, in situ direct laser deposition was used to additively manufacture three-dimensional NiTi structures from Ni and Ti powders. The mechanical property of NiTi has been shown to be similar to bone. Nanosecond pulsed laser sintering process was then utilized to generate a nanoporous composite surface with NiTi alloy and hydroxyapatite (HA) by ultrafast laser heating and cooling of Ni, Ti, and HA nanoparticles mixtures precoated on the 3D NiTi substrates; HA was added in order to improve the biocompatibility of the alloy. We then studied the underlying mechanism in the formation of NiTi/HA nanocomposite, and the synergistic effect of the sintered HA component and the nanoporous topology of the composite coating. In addition, we examined the activity of bone-forming osteoblasts on the NiTi/HA surfaces. For this, osteoblast cell morphology and various biomarkers were examined to evaluate cellular activity and function.Results: We found that the nanoscale porosity delivered by nanosecond pulsed laser sintering and the HA component positively contributed to osteoblast differentiation, as indicated by an increase in the expression of collagen and alkaline phosphatase, both of which are necessary for osteoblast mineralization. In addition, we observed topological complexities which appeared to boost the activity of osteoblasts, including an increase in actin cytoskeletal structures and adhesion structures.Conclusion: These findings demonstrate that the pulsed laser sintering method is an effective tool to generate biocompatible coatings in complex alloy-composite material systems with desired composition and topology. Our findings also provide a better understanding of the osteoinductive behavior of the sintered nanocomposite coatings for use in orthopedic and bone regeneration applications. Keywords: metal implants, bone regeneration, osteoinductive behavior, pulsed laser coating, biocompatibility, differentiation, alkaline phosphatase
format	article
author	Deng B Bruzzaniti A Cheng GJ
author_facet	Deng B Bruzzaniti A Cheng GJ
author_sort	Deng B
title	Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
title_short	Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
title_full	Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
title_fullStr	Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
title_full_unstemmed	Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering
title_sort	enhancement of osteoblast activity on nanostructured niti/hydroxyapatite coatings on additive manufactured niti metal implants by nanosecond pulsed laser sintering
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c2d470a2e5ad4fa29c36814625dc7974
work_keys_str_mv	AT dengb enhancementofosteoblastactivityonnanostructurednitihydroxyapatitecoatingsonadditivemanufacturednitimetalimplantsbynanosecondpulsedlasersintering AT bruzzanitia enhancementofosteoblastactivityonnanostructurednitihydroxyapatitecoatingsonadditivemanufacturednitimetalimplantsbynanosecondpulsedlasersintering AT chenggj enhancementofosteoblastactivityonnanostructurednitihydroxyapatitecoatingsonadditivemanufacturednitimetalimplantsbynanosecondpulsedlasersintering
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Enhancement of osteoblast activity on nanostructured NiTi/hydroxyapatite coatings on additive manufactured NiTi metal implants by nanosecond pulsed laser sintering

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