The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina

Abstract The fovea is a pit formed in the center of the retina that enables high-acuity vision in certain vertebrate species. While formation of the fovea fascinates many researchers, the molecular mechanisms underlying foveal development are poorly understood. In the current study, we histologicall...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takefumi Sugiyama, Haruka Yamamoto, Tetsuo Kon, Taro Chaya, Yoshihiro Omori, Yutaka Suzuki, Kentaro Abe, Dai Watanabe, Takahisa Furukawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2dc0014cf1b47188cc42860b4fdf674
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c2dc0014cf1b47188cc42860b4fdf674
record_format	dspace
spelling	oai:doaj.org-article:c2dc0014cf1b47188cc42860b4fdf6742021-12-02T16:18:06ZThe potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina10.1038/s41598-020-78452-62045-2322https://doaj.org/article/c2dc0014cf1b47188cc42860b4fdf6742020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78452-6https://doaj.org/toc/2045-2322Abstract The fovea is a pit formed in the center of the retina that enables high-acuity vision in certain vertebrate species. While formation of the fovea fascinates many researchers, the molecular mechanisms underlying foveal development are poorly understood. In the current study, we histologically investigated foveal development in zebra finch (Taeniopygia guttata) and found that foveal pit formation begins just before post-hatch day 14 (P14). We next performed RNA-seq analysis to compare gene expression profiles between the central (foveal and parafoveal) and peripheral retina in zebra finch at P14. We found that the Arhgef33 expression is enriched in the middle layer of the inner nuclear layer at the parafovea, suggesting that Arhgef33 is dominantly expressed in Müller glial cells in the developing parafovea. We then performed a pull-down assay using Rhotekin-RBD and observed GEF activity of Arhgef33 against RhoA. We found that overexpression of Arhgef33 in HEK293 cells induces cell contraction and that Arhgef33 expression inhibits neurite extension in Neuro 2A cells, which is partially recovered by a Rho-kinase (ROCK) inhibitor. Taken together, we used zebra finch as a model animal to investigate foveal development and identified Arhgef33 as a candidate protein possibly involved in foveal development through modulating RhoA activity.Takefumi SugiyamaHaruka YamamotoTetsuo KonTaro ChayaYoshihiro OmoriYutaka SuzukiKentaro AbeDai WatanabeTakahisa FurukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takefumi Sugiyama Haruka Yamamoto Tetsuo Kon Taro Chaya Yoshihiro Omori Yutaka Suzuki Kentaro Abe Dai Watanabe Takahisa Furukawa The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
description	Abstract The fovea is a pit formed in the center of the retina that enables high-acuity vision in certain vertebrate species. While formation of the fovea fascinates many researchers, the molecular mechanisms underlying foveal development are poorly understood. In the current study, we histologically investigated foveal development in zebra finch (Taeniopygia guttata) and found that foveal pit formation begins just before post-hatch day 14 (P14). We next performed RNA-seq analysis to compare gene expression profiles between the central (foveal and parafoveal) and peripheral retina in zebra finch at P14. We found that the Arhgef33 expression is enriched in the middle layer of the inner nuclear layer at the parafovea, suggesting that Arhgef33 is dominantly expressed in Müller glial cells in the developing parafovea. We then performed a pull-down assay using Rhotekin-RBD and observed GEF activity of Arhgef33 against RhoA. We found that overexpression of Arhgef33 in HEK293 cells induces cell contraction and that Arhgef33 expression inhibits neurite extension in Neuro 2A cells, which is partially recovered by a Rho-kinase (ROCK) inhibitor. Taken together, we used zebra finch as a model animal to investigate foveal development and identified Arhgef33 as a candidate protein possibly involved in foveal development through modulating RhoA activity.
format	article
author	Takefumi Sugiyama Haruka Yamamoto Tetsuo Kon Taro Chaya Yoshihiro Omori Yutaka Suzuki Kentaro Abe Dai Watanabe Takahisa Furukawa
author_facet	Takefumi Sugiyama Haruka Yamamoto Tetsuo Kon Taro Chaya Yoshihiro Omori Yutaka Suzuki Kentaro Abe Dai Watanabe Takahisa Furukawa
author_sort	Takefumi Sugiyama
title	The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
title_short	The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
title_full	The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
title_fullStr	The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
title_full_unstemmed	The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina
title_sort	potential role of arhgef33 rhogef in foveal development in the zebra finch retina
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c2dc0014cf1b47188cc42860b4fdf674
work_keys_str_mv	AT takefumisugiyama thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT harukayamamoto thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT tetsuokon thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT tarochaya thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT yoshihiroomori thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT yutakasuzuki thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT kentaroabe thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT daiwatanabe thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT takahisafurukawa thepotentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT takefumisugiyama potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT harukayamamoto potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT tetsuokon potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT tarochaya potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT yoshihiroomori potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT yutakasuzuki potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT kentaroabe potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT daiwatanabe potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina AT takahisafurukawa potentialroleofarhgef33rhogefinfovealdevelopmentinthezebrafinchretina
_version_	1718384198067159040

The potential role of Arhgef33 RhoGEF in foveal development in the zebra finch retina

Ejemplares similares