The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation

Abstract Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths....

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Autores principales:	Ejun (Elijah) Huang, Enzo Tedone, Ryan O’Hara, Crystal Cornelius, Tsung-Po Lai, Andrew Ludlow, Woodring E. Wright, Jerry W. Shay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2dc49f6b23047739c01bec8a08dc03e
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spelling	oai:doaj.org-article:c2dc49f6b23047739c01bec8a08dc03e2021-12-02T12:32:55ZThe Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation10.1038/s41598-017-05174-72045-2322https://doaj.org/article/c2dc49f6b23047739c01bec8a08dc03e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05174-7https://doaj.org/toc/2045-2322Abstract Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths. In this study, we found that telomerase activation is not required for the short- term proliferation of T cells and that telomeres progressively shorten in a heterogeneous population of T cells, even if telomerase is detected. By measuring telomerase activity at the single-cell level using quantitative ddPCR techniques (ddTRAP) and by monitoring changes in the shortest telomeres with more sensitive telomere length measurement assays, we show that only a subset of CD28+ T-cells have robust telomerase activity upon stimulation and are capable of maintaining their telomere lengths during induced proliferation. The study of this T-cell subset may lead to a better understanding on how telomerase is regulated and functions in immune cells.Ejun (Elijah) HuangEnzo TedoneRyan O’HaraCrystal CorneliusTsung-Po LaiAndrew LudlowWoodring E. WrightJerry W. ShayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ejun (Elijah) Huang Enzo Tedone Ryan O’Hara Crystal Cornelius Tsung-Po Lai Andrew Ludlow Woodring E. Wright Jerry W. Shay The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
description	Abstract Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths. In this study, we found that telomerase activation is not required for the short- term proliferation of T cells and that telomeres progressively shorten in a heterogeneous population of T cells, even if telomerase is detected. By measuring telomerase activity at the single-cell level using quantitative ddPCR techniques (ddTRAP) and by monitoring changes in the shortest telomeres with more sensitive telomere length measurement assays, we show that only a subset of CD28+ T-cells have robust telomerase activity upon stimulation and are capable of maintaining their telomere lengths during induced proliferation. The study of this T-cell subset may lead to a better understanding on how telomerase is regulated and functions in immune cells.
format	article
author	Ejun (Elijah) Huang Enzo Tedone Ryan O’Hara Crystal Cornelius Tsung-Po Lai Andrew Ludlow Woodring E. Wright Jerry W. Shay
author_facet	Ejun (Elijah) Huang Enzo Tedone Ryan O’Hara Crystal Cornelius Tsung-Po Lai Andrew Ludlow Woodring E. Wright Jerry W. Shay
author_sort	Ejun (Elijah) Huang
title	The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
title_short	The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
title_full	The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
title_fullStr	The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
title_full_unstemmed	The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
title_sort	maintenance of telomere length in cd28+ t cells during t lymphocyte stimulation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c2dc49f6b23047739c01bec8a08dc03e
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The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation

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