Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation

Abstract To investigate the ability of theaflavins (TF) from black tea to protect dentin collagen against enzymatic degradation via cross-linking effect under clinically relevant conditions. 10-µm-thick dentin films were microtomed from dentin slabs of human molars. Following demineralization, films...

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Autores principales:	Hang Liu, Jing Guo, Rong Wang, Yong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c2e78a0e037c4d8cb76b78cf49e53fa9
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spelling	oai:doaj.org-article:c2e78a0e037c4d8cb76b78cf49e53fa92021-12-02T18:01:48ZTheaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation10.1038/s41598-021-99186-z2045-2322https://doaj.org/article/c2e78a0e037c4d8cb76b78cf49e53fa92021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99186-zhttps://doaj.org/toc/2045-2322Abstract To investigate the ability of theaflavins (TF) from black tea to protect dentin collagen against enzymatic degradation via cross-linking effect under clinically relevant conditions. 10-µm-thick dentin films were microtomed from dentin slabs of human molars. Following demineralization, films or slabs were treated with TF at two concentrations (0.4% and 2%) for 30 s. A well-known collagen cross-linker grape seed proanthocyanidins (PA) was used as control. Collagen cross-linking interactions and stabilization against enzymatic degradation were investigated by Fourier transform infrared spectroscopy, weight loss, hydroxyproline release, and scanning/transmission electron microscopy. Data were analyzed by ANOVA, Tukey’s and Student’s T test (α = 0.05%). The results showed collagen cross-linking and stabilization efficacy was dependent on TF/PA concentrations. At 2.0%, TF and PA offered nearly full protection to collagen; at 0.4%, TF exhibited a significantly better collagen stabilization effect than PA (P < 0.05), while untreated collagen was completely digested. It’s concluded that TF cross-links dentin collagen within a clinically relevant time (30 s) and offers excellent collagen protection against enzymatic degradation, with efficacy comparable to or better than PA. The study supports the potential use of TF as a novel, promising collagen cross-linker for degradation resistant, long-lasting dentin bonding in composite restorations.Hang LiuJing GuoRong WangYong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hang Liu Jing Guo Rong Wang Yong Wang Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
description	Abstract To investigate the ability of theaflavins (TF) from black tea to protect dentin collagen against enzymatic degradation via cross-linking effect under clinically relevant conditions. 10-µm-thick dentin films were microtomed from dentin slabs of human molars. Following demineralization, films or slabs were treated with TF at two concentrations (0.4% and 2%) for 30 s. A well-known collagen cross-linker grape seed proanthocyanidins (PA) was used as control. Collagen cross-linking interactions and stabilization against enzymatic degradation were investigated by Fourier transform infrared spectroscopy, weight loss, hydroxyproline release, and scanning/transmission electron microscopy. Data were analyzed by ANOVA, Tukey’s and Student’s T test (α = 0.05%). The results showed collagen cross-linking and stabilization efficacy was dependent on TF/PA concentrations. At 2.0%, TF and PA offered nearly full protection to collagen; at 0.4%, TF exhibited a significantly better collagen stabilization effect than PA (P < 0.05), while untreated collagen was completely digested. It’s concluded that TF cross-links dentin collagen within a clinically relevant time (30 s) and offers excellent collagen protection against enzymatic degradation, with efficacy comparable to or better than PA. The study supports the potential use of TF as a novel, promising collagen cross-linker for degradation resistant, long-lasting dentin bonding in composite restorations.
format	article
author	Hang Liu Jing Guo Rong Wang Yong Wang
author_facet	Hang Liu Jing Guo Rong Wang Yong Wang
author_sort	Hang Liu
title	Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
title_short	Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
title_full	Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
title_fullStr	Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
title_full_unstemmed	Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
title_sort	theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c2e78a0e037c4d8cb76b78cf49e53fa9
work_keys_str_mv	AT hangliu theaflavinsasanovelcrosslinkerquicklystabilizedemineralizeddentincollagenagainstdegradation AT jingguo theaflavinsasanovelcrosslinkerquicklystabilizedemineralizeddentincollagenagainstdegradation AT rongwang theaflavinsasanovelcrosslinkerquicklystabilizedemineralizeddentincollagenagainstdegradation AT yongwang theaflavinsasanovelcrosslinkerquicklystabilizedemineralizeddentincollagenagainstdegradation
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Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation

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