Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis

In order to investigate the expression of lncRNAs in the ovaries of Qira black sheep at different stages of the estrous cycle, Qira black sheep were used as experimental materials in this experiment, and after estrus synchronization, ovarian tissues at four different stages of the estrous cycle were...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xi CHEN, Hanying CHEN, Song JIANG, Hong SHEN, Xiancun ZENG
Formato:	article
Lenguaje:	EN
Publicado:	University of Kafkas 2021
Materias:	estrous ovary lncrna qira black sheep Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/c2f66cdb8406459bb7f3746b62f33467
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c2f66cdb8406459bb7f3746b62f33467
record_format	dspace
spelling	oai:doaj.org-article:c2f66cdb8406459bb7f3746b62f334672021-12-05T08:21:55ZIdentification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis10.9775/kvfd.2021.262031300-60451309-2251https://doaj.org/article/c2f66cdb8406459bb7f3746b62f334672021-11-01T00:00:00Zhttp://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2853.pdfhttps://doaj.org/toc/1300-6045https://doaj.org/toc/1309-2251In order to investigate the expression of lncRNAs in the ovaries of Qira black sheep at different stages of the estrous cycle, Qira black sheep were used as experimental materials in this experiment, and after estrus synchronization, ovarian tissues at four different stages of the estrous cycle were collected to extract total RNA.The samples at different stages were first subjected to genome-wide analysis using RNA-seq technology; target genes of lncRNAs were predicted using co-expression methods; and then GO and KEGG analysis of target genes was performed. Genes related to the estrous cycle of Qira black sheep were also selected to study their transcriptional differences. Finally, lncRNA-miRNA and mRNAlncRNA-miRNA interaction networks were established to further analyze the effect of lncRNA alignment on Qira black sheep reproduction. The results showed that in the differentially expressed part, 14 lncRNAs were differentially expressed in Estrous VS Diestrus; the differential expression levels of lncRNAs in the two comparison groups of Estrous VS Metestrus and Estrus VS Proestrus were 18 and 24, respectively. The results of GO and KEGG functional enrichment analysis showed that differentially expressed lncRNAs and their target genes were mainly involved in reproductionrelated pathways such as retinol metabolism, ovarian sterol production and endosterol biosynthesis. In the combined analysis of lncRNA-miRNA and mRNA-lncRNA-miRNA, genes related to reproduction, such as LNC011583, LNC003443 and bta-miR-202, were found, thus it can be seen that lncRNAs have some effect in the reproduction of Qira black sheep.Xi CHENHanying CHENSong JIANGHong SHENXiancun ZENGUniversity of Kafkasarticleestrousovarylncrnaqira black sheepVeterinary medicineSF600-1100ENKafkas Universitesi Veteriner Fakultesi Dergisi, Pp 733-740 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	estrous ovary lncrna qira black sheep Veterinary medicine SF600-1100
spellingShingle	estrous ovary lncrna qira black sheep Veterinary medicine SF600-1100 Xi CHEN Hanying CHEN Song JIANG Hong SHEN Xiancun ZENG Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
description	In order to investigate the expression of lncRNAs in the ovaries of Qira black sheep at different stages of the estrous cycle, Qira black sheep were used as experimental materials in this experiment, and after estrus synchronization, ovarian tissues at four different stages of the estrous cycle were collected to extract total RNA.The samples at different stages were first subjected to genome-wide analysis using RNA-seq technology; target genes of lncRNAs were predicted using co-expression methods; and then GO and KEGG analysis of target genes was performed. Genes related to the estrous cycle of Qira black sheep were also selected to study their transcriptional differences. Finally, lncRNA-miRNA and mRNAlncRNA-miRNA interaction networks were established to further analyze the effect of lncRNA alignment on Qira black sheep reproduction. The results showed that in the differentially expressed part, 14 lncRNAs were differentially expressed in Estrous VS Diestrus; the differential expression levels of lncRNAs in the two comparison groups of Estrous VS Metestrus and Estrus VS Proestrus were 18 and 24, respectively. The results of GO and KEGG functional enrichment analysis showed that differentially expressed lncRNAs and their target genes were mainly involved in reproductionrelated pathways such as retinol metabolism, ovarian sterol production and endosterol biosynthesis. In the combined analysis of lncRNA-miRNA and mRNA-lncRNA-miRNA, genes related to reproduction, such as LNC011583, LNC003443 and bta-miR-202, were found, thus it can be seen that lncRNAs have some effect in the reproduction of Qira black sheep.
format	article
author	Xi CHEN Hanying CHEN Song JIANG Hong SHEN Xiancun ZENG
author_facet	Xi CHEN Hanying CHEN Song JIANG Hong SHEN Xiancun ZENG
author_sort	Xi CHEN
title	Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
title_short	Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
title_full	Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
title_fullStr	Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
title_full_unstemmed	Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis
title_sort	identification of lncrna expression in the estrous cycle of qira black sheep and its combination with mirna analysis
publisher	University of Kafkas
publishDate	2021
url	https://doaj.org/article/c2f66cdb8406459bb7f3746b62f33467
work_keys_str_mv	AT xichen identificationoflncrnaexpressionintheestrouscycleofqirablacksheepanditscombinationwithmirnaanalysis AT hanyingchen identificationoflncrnaexpressionintheestrouscycleofqirablacksheepanditscombinationwithmirnaanalysis AT songjiang identificationoflncrnaexpressionintheestrouscycleofqirablacksheepanditscombinationwithmirnaanalysis AT hongshen identificationoflncrnaexpressionintheestrouscycleofqirablacksheepanditscombinationwithmirnaanalysis AT xiancunzeng identificationoflncrnaexpressionintheestrouscycleofqirablacksheepanditscombinationwithmirnaanalysis
_version_	1718372582235832320

Identification of LncRNA Expression in the Estrous Cycle of Qira Black Sheep and Its Combination with miRNA Analysis

Ejemplares similares