Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

ABSTRACT When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the perf...

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Bibliographic Details
Main Authors: Q. R. Ducarmon, B. V. H. Hornung, A. R. Geelen, E. J. Kuijper, R. D. Zwittink
Format: article
Language:EN
Published: American Society for Microbiology 2020
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Online Access:https://doaj.org/article/c2fbe2ccd157439fb50811695e35b20b
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