The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression

Osteoporosis is a complex multifactorial disorder linked to various risk factors and medical conditions. Bone marrow-derived mesenchymal stem cell (BMSC) dysfunction potentially plays a critical role in osteoporosis pathogenesis. Herein, the study identified that miR-4739 was upregulated in BMSC cul...

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Autores principales:	Ding Li, Qi Yuan, Liang Xiong, Aoyu Li, Yu Xia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Osteoporosis bone marrow-derived mesenchymal stem cell (BMSC) osteogenic differentiation miR-4739 DLX3 Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/c2fec2bf50594487ad50dea64a6b4e52
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spelling	oai:doaj.org-article:c2fec2bf50594487ad50dea64a6b4e522021-12-03T08:46:20ZThe miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression1664-239210.3389/fendo.2021.703167https://doaj.org/article/c2fec2bf50594487ad50dea64a6b4e522021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.703167/fullhttps://doaj.org/toc/1664-2392Osteoporosis is a complex multifactorial disorder linked to various risk factors and medical conditions. Bone marrow-derived mesenchymal stem cell (BMSC) dysfunction potentially plays a critical role in osteoporosis pathogenesis. Herein, the study identified that miR-4739 was upregulated in BMSC cultures harvested from osteoporotic subjects. BMSCs were isolated from normal and osteoporotic bone marrow tissues and identified for their osteogenic differentiation potential. In osteoporotic BMSCs, miR-4739 overexpression significantly inhibited cell viability, osteoblast differentiation, mineralized nodule formation, and heterotopic bone formation, whereas miR-4739 inhibition exerted opposite effects. Through direct binding, miR-4739 inhibited distal-less homeobox 3 (DLX3) expression. In osteoporotic BMSCs, DLX3 knockdown also inhibited BMSC viability and osteogenic differentiation. Moreover, DLX3 knockdown partially attenuated the effects of miR-4739 inhibition upon BMSCs. Altogether, the miR-4739/DLX3 axis modulates the capacity of BMSCs to differentiate into osteoblasts, which potentially plays a role in osteoporosis pathogenesis. The in vivo and clinical functions of the miR-4739/DLX3 axis require further investigation.Ding LiQi YuanLiang XiongAoyu LiYu XiaFrontiers Media S.A.articleOsteoporosisbone marrow-derived mesenchymal stem cell (BMSC)osteogenic differentiationmiR-4739DLX3Diseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteoporosis bone marrow-derived mesenchymal stem cell (BMSC) osteogenic differentiation miR-4739 DLX3 Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	Osteoporosis bone marrow-derived mesenchymal stem cell (BMSC) osteogenic differentiation miR-4739 DLX3 Diseases of the endocrine glands. Clinical endocrinology RC648-665 Ding Li Qi Yuan Liang Xiong Aoyu Li Yu Xia The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
description	Osteoporosis is a complex multifactorial disorder linked to various risk factors and medical conditions. Bone marrow-derived mesenchymal stem cell (BMSC) dysfunction potentially plays a critical role in osteoporosis pathogenesis. Herein, the study identified that miR-4739 was upregulated in BMSC cultures harvested from osteoporotic subjects. BMSCs were isolated from normal and osteoporotic bone marrow tissues and identified for their osteogenic differentiation potential. In osteoporotic BMSCs, miR-4739 overexpression significantly inhibited cell viability, osteoblast differentiation, mineralized nodule formation, and heterotopic bone formation, whereas miR-4739 inhibition exerted opposite effects. Through direct binding, miR-4739 inhibited distal-less homeobox 3 (DLX3) expression. In osteoporotic BMSCs, DLX3 knockdown also inhibited BMSC viability and osteogenic differentiation. Moreover, DLX3 knockdown partially attenuated the effects of miR-4739 inhibition upon BMSCs. Altogether, the miR-4739/DLX3 axis modulates the capacity of BMSCs to differentiate into osteoblasts, which potentially plays a role in osteoporosis pathogenesis. The in vivo and clinical functions of the miR-4739/DLX3 axis require further investigation.
format	article
author	Ding Li Qi Yuan Liang Xiong Aoyu Li Yu Xia
author_facet	Ding Li Qi Yuan Liang Xiong Aoyu Li Yu Xia
author_sort	Ding Li
title	The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
title_short	The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
title_full	The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
title_fullStr	The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
title_full_unstemmed	The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression
title_sort	mir-4739/dlx3 axis modulates bone marrow-derived mesenchymal stem cell (bmsc) osteogenesis affecting osteoporosis progression
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c2fec2bf50594487ad50dea64a6b4e52
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The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression

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