Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities

Moayad Alhariri,1 Majed A Majrashi,2 Ali H Bahkali,3 Faisal S Almajed,4 Ali O Azghani,5 Mohammad A Khiyami,2 Essam J Alyamani,2 Sameera M Aljohani,6 Majed A Halwani1 1Nanomedicine Department, King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health...

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Autores principales:	Alhariri M, Majrashi MA, Bahkali AH, Almajed FS, Azghani AO, Khiyami MA, Alyamani EJ, Aljohani SM, Halwani MA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Drug delivery stability antibacterial activity and biofilm Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c303f57dbd854f7691bb14923f890daf
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spelling	oai:doaj.org-article:c303f57dbd854f7691bb14923f890daf2021-12-02T07:28:29ZEfficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities1178-2013https://doaj.org/article/c303f57dbd854f7691bb14923f890daf2017-09-01T00:00:00Zhttps://www.dovepress.com/efficacy-of-neutral-and-negatively-charged-liposome-loaded-gentamicin--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Moayad Alhariri,1 Majed A Majrashi,2 Ali H Bahkali,3 Faisal S Almajed,4 Ali O Azghani,5 Mohammad A Khiyami,2 Essam J Alyamani,2 Sameera M Aljohani,6 Majed A Halwani1 1Nanomedicine Department, King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, 2National Centre for Biotechnology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), 3Botany and Microbiology Department, College of Science, King Saud University, 4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia; 5Department of Biology, The University of Texas at Tyler, Tyler, TX, USA; 6College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia Abstract: We investigated the efficacy of liposomal gentamicin formulations of different surface charges against Pseudomonas aeruginosa and Klebsiella oxytoca. The liposomal gentamicin formulations were prepared by the dehydration–rehydration method, and their sizes and zeta potential were measured. Gentamicin encapsulation efficiency inside the liposomal formulations was determined by microbiologic assay, and stability of the formulations in biologic fluid was evaluated for a period of 48 h. The minimum inhibitory concentration and the minimum bactericidal concentration were determined, and the in vitro time kill studies of the free form of gentamicin and liposomal gentamicin formulations were performed. The activities of liposomal gentamicin in preventing and reducing biofilm-forming P. aeruginosa and K. oxytoca were compared to those of free antibiotic. The sizes of the liposomal formulations ranged from 625 to 806.6 nm in diameter, with the zeta potential ranging from –0.22 to –31.7 mV. Gentamicin encapsulation efficiency inside the liposomal formulation ranged from 1.8% to 43.6%. The liposomes retained >60% of their gentamicin content during the 48 h time period. The minimum inhibitory concentration of neutral formulation was lower than that of free gentamicin (0.25 versus 1 mg/L for P. aeruginosa and 0.5 versus 1 mg/L for K. oxytoca). The negatively charged formulation exhibited the same bacteriostatic concentration as that of free gentamicin. The minimum bactericidal concentration of neutral liposomes on planktonic bacterial culture was twofold lower than that of free gentamicin, whereas the negatively charged formulations were comparable to free gentamicin. The killing time curve values for the neutral negatively charged formulation against planktonic P. aeruginosa and K. oxytoca were better than those of free gentamicin. Furthermore, liposomal formulations prevent the biofilm-formation ability of these strains better than free gentamicin. In summary, liposomal formulations could be an effective lipid nanoparticle to combat acute infections where planktonic bacteria are predominant. Keywords: drug delivery, stability, antibacterial activity, biofilmAlhariri MMajrashi MABahkali AHAlmajed FSAzghani AOKhiyami MAAlyamani EJAljohani SMHalwani MADove Medical PressarticleDrug deliverystabilityantibacterial activityand biofilmMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6949-6961 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Drug delivery stability antibacterial activity and biofilm Medicine (General) R5-920
spellingShingle	Drug delivery stability antibacterial activity and biofilm Medicine (General) R5-920 Alhariri M Majrashi MA Bahkali AH Almajed FS Azghani AO Khiyami MA Alyamani EJ Aljohani SM Halwani MA Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
description	Moayad Alhariri,1 Majed A Majrashi,2 Ali H Bahkali,3 Faisal S Almajed,4 Ali O Azghani,5 Mohammad A Khiyami,2 Essam J Alyamani,2 Sameera M Aljohani,6 Majed A Halwani1 1Nanomedicine Department, King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, 2National Centre for Biotechnology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), 3Botany and Microbiology Department, College of Science, King Saud University, 4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia; 5Department of Biology, The University of Texas at Tyler, Tyler, TX, USA; 6College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia Abstract: We investigated the efficacy of liposomal gentamicin formulations of different surface charges against Pseudomonas aeruginosa and Klebsiella oxytoca. The liposomal gentamicin formulations were prepared by the dehydration–rehydration method, and their sizes and zeta potential were measured. Gentamicin encapsulation efficiency inside the liposomal formulations was determined by microbiologic assay, and stability of the formulations in biologic fluid was evaluated for a period of 48 h. The minimum inhibitory concentration and the minimum bactericidal concentration were determined, and the in vitro time kill studies of the free form of gentamicin and liposomal gentamicin formulations were performed. The activities of liposomal gentamicin in preventing and reducing biofilm-forming P. aeruginosa and K. oxytoca were compared to those of free antibiotic. The sizes of the liposomal formulations ranged from 625 to 806.6 nm in diameter, with the zeta potential ranging from –0.22 to –31.7 mV. Gentamicin encapsulation efficiency inside the liposomal formulation ranged from 1.8% to 43.6%. The liposomes retained >60% of their gentamicin content during the 48 h time period. The minimum inhibitory concentration of neutral formulation was lower than that of free gentamicin (0.25 versus 1 mg/L for P. aeruginosa and 0.5 versus 1 mg/L for K. oxytoca). The negatively charged formulation exhibited the same bacteriostatic concentration as that of free gentamicin. The minimum bactericidal concentration of neutral liposomes on planktonic bacterial culture was twofold lower than that of free gentamicin, whereas the negatively charged formulations were comparable to free gentamicin. The killing time curve values for the neutral negatively charged formulation against planktonic P. aeruginosa and K. oxytoca were better than those of free gentamicin. Furthermore, liposomal formulations prevent the biofilm-formation ability of these strains better than free gentamicin. In summary, liposomal formulations could be an effective lipid nanoparticle to combat acute infections where planktonic bacteria are predominant. Keywords: drug delivery, stability, antibacterial activity, biofilm
format	article
author	Alhariri M Majrashi MA Bahkali AH Almajed FS Azghani AO Khiyami MA Alyamani EJ Aljohani SM Halwani MA
author_facet	Alhariri M Majrashi MA Bahkali AH Almajed FS Azghani AO Khiyami MA Alyamani EJ Aljohani SM Halwani MA
author_sort	Alhariri M
title	Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
title_short	Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
title_full	Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
title_fullStr	Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
title_full_unstemmed	Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
title_sort	efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/c303f57dbd854f7691bb14923f890daf
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Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities

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