Effects of muscle relaxants on ischaemia damage in skeletal muscle

Abstract Muscle ischaemia is frequently induced intraoperatively by i.e. a surgical tourniquet or during the re-grafting phase of a free muscle transplant. The resulting muscle cell damage may impact on postoperative recovery. Neuromuscular paralysis may mitigate the effects of ischaemia. After ethi...

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Autores principales:	Thomas Ledowski, Simone Nißler, Manuel Wenk, Esther M. Pogatzki-Zahn, Daniel Segelcke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c305599c37d0478395b3bbbc2f014bc2
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spelling	oai:doaj.org-article:c305599c37d0478395b3bbbc2f014bc22021-12-02T11:40:24ZEffects of muscle relaxants on ischaemia damage in skeletal muscle10.1038/s41598-018-24127-22045-2322https://doaj.org/article/c305599c37d0478395b3bbbc2f014bc22018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24127-2https://doaj.org/toc/2045-2322Abstract Muscle ischaemia is frequently induced intraoperatively by i.e. a surgical tourniquet or during the re-grafting phase of a free muscle transplant. The resulting muscle cell damage may impact on postoperative recovery. Neuromuscular paralysis may mitigate the effects of ischaemia. After ethics approval, 25 male Sprague-Dawley rats were anaesthetized and randomly assigned to 1 of 4 groups: Sham operation, treatment with normal saline, treatment with rocuronium (muscle relaxant) 0.6 or 1 mg kg−1, respectively. In the non-sham groups, ischaemia of one hind leg was achieved by ligation of the femoral vessels. Muscle biopsies were taken at 30 and 90 min, respectively. Cell damage was assessed in the biopsies via the expression of dystrophin, free calcium, as well as the assessment of cell viability. Pre-ischaemia muscle relaxation led to a reduction in ischaemia-induced muscle cell damage when measured by the expression of dystrophin, cell viability and the expression of free calcium even after 90 min of ischaemia (i.e. ratio control/ischaemic site for dystrophin expression after saline 0.58 ± 0.12 vs. after 1 mg/kg rocuronium 1.08 ± 0.29; P < 0.05). Muscle relaxation decreased the degree of ischaemia-induced muscle cell damage. The results may have significant clinical implications.Thomas LedowskiSimone NißlerManuel WenkEsther M. Pogatzki-ZahnDaniel SegelckeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Ledowski Simone Nißler Manuel Wenk Esther M. Pogatzki-Zahn Daniel Segelcke Effects of muscle relaxants on ischaemia damage in skeletal muscle
description	Abstract Muscle ischaemia is frequently induced intraoperatively by i.e. a surgical tourniquet or during the re-grafting phase of a free muscle transplant. The resulting muscle cell damage may impact on postoperative recovery. Neuromuscular paralysis may mitigate the effects of ischaemia. After ethics approval, 25 male Sprague-Dawley rats were anaesthetized and randomly assigned to 1 of 4 groups: Sham operation, treatment with normal saline, treatment with rocuronium (muscle relaxant) 0.6 or 1 mg kg−1, respectively. In the non-sham groups, ischaemia of one hind leg was achieved by ligation of the femoral vessels. Muscle biopsies were taken at 30 and 90 min, respectively. Cell damage was assessed in the biopsies via the expression of dystrophin, free calcium, as well as the assessment of cell viability. Pre-ischaemia muscle relaxation led to a reduction in ischaemia-induced muscle cell damage when measured by the expression of dystrophin, cell viability and the expression of free calcium even after 90 min of ischaemia (i.e. ratio control/ischaemic site for dystrophin expression after saline 0.58 ± 0.12 vs. after 1 mg/kg rocuronium 1.08 ± 0.29; P < 0.05). Muscle relaxation decreased the degree of ischaemia-induced muscle cell damage. The results may have significant clinical implications.
format	article
author	Thomas Ledowski Simone Nißler Manuel Wenk Esther M. Pogatzki-Zahn Daniel Segelcke
author_facet	Thomas Ledowski Simone Nißler Manuel Wenk Esther M. Pogatzki-Zahn Daniel Segelcke
author_sort	Thomas Ledowski
title	Effects of muscle relaxants on ischaemia damage in skeletal muscle
title_short	Effects of muscle relaxants on ischaemia damage in skeletal muscle
title_full	Effects of muscle relaxants on ischaemia damage in skeletal muscle
title_fullStr	Effects of muscle relaxants on ischaemia damage in skeletal muscle
title_full_unstemmed	Effects of muscle relaxants on ischaemia damage in skeletal muscle
title_sort	effects of muscle relaxants on ischaemia damage in skeletal muscle
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c305599c37d0478395b3bbbc2f014bc2
work_keys_str_mv	AT thomasledowski effectsofmusclerelaxantsonischaemiadamageinskeletalmuscle AT simonenißler effectsofmusclerelaxantsonischaemiadamageinskeletalmuscle AT manuelwenk effectsofmusclerelaxantsonischaemiadamageinskeletalmuscle AT esthermpogatzkizahn effectsofmusclerelaxantsonischaemiadamageinskeletalmuscle AT danielsegelcke effectsofmusclerelaxantsonischaemiadamageinskeletalmuscle
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Effects of muscle relaxants on ischaemia damage in skeletal muscle

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