Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats

Melanin concentrating hormone (MCH) is a sleep-promoting neuromodulator synthesized by neurons located in the postero-lateral hypothalamus and incerto-hypothalamic area. MCHergic neurons have widespread projections including the serotonergic dorsal (DR) and median (MnR) raphe nuclei, both involved i...

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Autores principales:	Claudia Pascovich, Soﬁa Niño, Alejandra Mondino, Ximena Lopez-Hill, Jessika Urbanavicius, Jaime Monti, Patricia Lagos, Pablo Torterolo
Formato:	article
Lenguaje:	EN
Publicado:	Brazilian Association of Sleep and Latin American Federation of Sleep Societies 2021
Materias:	serotonin hypothalamus sleep, rem peptide paradoxical sleep slow wave sleep Psychology BF1-990 Consciousness. Cognition BF309-499
Acceso en línea:	https://doaj.org/article/c312142cb743488b92e93c714fd1206c
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spelling	oai:doaj.org-article:c312142cb743488b92e93c714fd1206c2021-11-24T12:26:11ZMicroinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats1984-06591984-0063000710.5935/1984-0063.20200075https://doaj.org/article/c312142cb743488b92e93c714fd1206c2021-10-01T00:00:00Zhttps://www.sleepscience.org.br/details/2829/en-US/microinjection-of-melanin-concentrating-hormone--mch--into-the-median-raphe-nucleus-promotes-rem-sleep-in-ratshttps://doaj.org/toc/1984-0659https://doaj.org/toc/1984-0063Melanin concentrating hormone (MCH) is a sleep-promoting neuromodulator synthesized by neurons located in the postero-lateral hypothalamus and incerto-hypothalamic area. MCHergic neurons have widespread projections including the serotonergic dorsal (DR) and median (MnR) raphe nuclei, both involved in the control of wakefulness and sleep. In the present study, we explored in rats the presence of the MCH receptor type 1 (MCHR-1) in serotonergic neurons of the MnR by double immunofluorescence. Additionally, we analyzed the effect on sleep of MCH microinjections into the MnR. We found that MCHR-1 protein was present in MnR serotonergic and non-serotonergic neurons. In this respect, the receptor was localized in the primary cilia of these neurons. Compared with saline, microinjections of MCH into the MnR induced a dose-related increase in REM sleep time, which was related to a rise in the number of REM sleep episodes, associated with a reduction in the time spent in W. No signiﬁcant changes were observed in non-REM (NREM) sleep time. Our data strongly suggest that MCH projections towards the MnR, acting through the MCHR-1 located in the primary cilia, promote REM sleep.Claudia PascovichSoﬁa NiñoAlejandra MondinoXimena Lopez-HillJessika UrbanaviciusJaime MontiPatricia LagosPablo TorteroloBrazilian Association of Sleep and Latin American Federation of Sleep Societies articleserotoninhypothalamussleep, rempeptideparadoxical sleepslow wave sleepPsychologyBF1-990Consciousness. CognitionBF309-499ENSleep Science, Vol 14, Iss 3, Pp 229-235 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	serotonin hypothalamus sleep, rem peptide paradoxical sleep slow wave sleep Psychology BF1-990 Consciousness. Cognition BF309-499
spellingShingle	serotonin hypothalamus sleep, rem peptide paradoxical sleep slow wave sleep Psychology BF1-990 Consciousness. Cognition BF309-499 Claudia Pascovich Soﬁa Niño Alejandra Mondino Ximena Lopez-Hill Jessika Urbanavicius Jaime Monti Patricia Lagos Pablo Torterolo Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
description	Melanin concentrating hormone (MCH) is a sleep-promoting neuromodulator synthesized by neurons located in the postero-lateral hypothalamus and incerto-hypothalamic area. MCHergic neurons have widespread projections including the serotonergic dorsal (DR) and median (MnR) raphe nuclei, both involved in the control of wakefulness and sleep. In the present study, we explored in rats the presence of the MCH receptor type 1 (MCHR-1) in serotonergic neurons of the MnR by double immunofluorescence. Additionally, we analyzed the effect on sleep of MCH microinjections into the MnR. We found that MCHR-1 protein was present in MnR serotonergic and non-serotonergic neurons. In this respect, the receptor was localized in the primary cilia of these neurons. Compared with saline, microinjections of MCH into the MnR induced a dose-related increase in REM sleep time, which was related to a rise in the number of REM sleep episodes, associated with a reduction in the time spent in W. No signiﬁcant changes were observed in non-REM (NREM) sleep time. Our data strongly suggest that MCH projections towards the MnR, acting through the MCHR-1 located in the primary cilia, promote REM sleep.
format	article
author	Claudia Pascovich Soﬁa Niño Alejandra Mondino Ximena Lopez-Hill Jessika Urbanavicius Jaime Monti Patricia Lagos Pablo Torterolo
author_facet	Claudia Pascovich Soﬁa Niño Alejandra Mondino Ximena Lopez-Hill Jessika Urbanavicius Jaime Monti Patricia Lagos Pablo Torterolo
author_sort	Claudia Pascovich
title	Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
title_short	Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
title_full	Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
title_fullStr	Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
title_full_unstemmed	Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats
title_sort	microinjection of melanin-concentrating hormone (mch) into the median raphe nucleus promotes rem sleep in rats
publisher	Brazilian Association of Sleep and Latin American Federation of Sleep Societies
publishDate	2021
url	https://doaj.org/article/c312142cb743488b92e93c714fd1206c
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Microinjection of melanin-concentrating hormone (MCH) into the median raphe nucleus promotes REM sleep in rats

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