Somatic Embryogenesis and Indirect In Vitro Plant Regeneration in Amorphophallus konjac K. Koch by One-Step Seedling Formation

Konjac (Amorphophallus konjac K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family Araceae, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the pl...

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Autores principales:	Dandan Li, Mohammad Aqa Mohammadi, Yuan Qin, Zongshen Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	organogenesis micro-corm in vitro regeneration plant growth regulations Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/c316c8b7b88147e5ae27cf8758535be4
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spelling	oai:doaj.org-article:c316c8b7b88147e5ae27cf8758535be42021-11-25T17:47:39ZSomatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation10.3390/horticulturae71104972311-7524https://doaj.org/article/c316c8b7b88147e5ae27cf8758535be42021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/497https://doaj.org/toc/2311-7524Konjac (<i>Amorphophallus konjac</i> K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family <i>Araceae</i>, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the plantlet regeneration efficiency of konjac using young leaves as an explant source. In the current study, we used several sterilization methods for tuber sterilization. Moreover, various plant growth regulator combinations were applied to achieve efficient somatic embryogenesis and plantlet regeneration. Our results showed that the optimal tuber sterilization was method C (75% alcohol for 15 s + 0.1% HgCl<sub>2</sub> for 15 min + washing by double-sterilized water three times). Three types of embryogenic calli were induced on full-strength Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L of 6-benzylaminopurine (6-BA), 0.5 mg/L of naphthaleneacetic acid (NAA), 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L of sucrose. Of the three types of embryogenic calli, only type Ⅲ further regenerated plantlets, with a callus induction rate of 55.73% and a seedling induction rate of 92.73%. This suggests that the addition of the above hormones gives the optimal callus induction. The proliferation rate achieved was 38% on the MS basal medium containing 1.0 mg/L of 6-BA, 1.0 mg/L of indolebutyric acid (IBA), 0.2 mg/L of kinetin (KT), and 50 g/L of sucrose. The one-step seedling formation achieved in MS medium contained 2.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0.1 mg/L of gibberellic acid (GA<sub>3</sub>), and 30 g/L of sucrose, and the number of regenerated shoots per explants was 6 ± 2. Therefore, we establish a one-step seedling regeneration system through indirect plant regeneration, which shortens the time for konjac in vitro regeneration, significantly increased the micropropagation efficiency, and decreased the cost of the konjac tissue culture.Dandan LiMohammad Aqa MohammadiYuan QinZongshen ZhangMDPI AGarticleorganogenesismicro-cormin vitro regenerationplant growth regulationsPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 497, p 497 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	organogenesis micro-corm in vitro regeneration plant growth regulations Plant culture SB1-1110
spellingShingle	organogenesis micro-corm in vitro regeneration plant growth regulations Plant culture SB1-1110 Dandan Li Mohammad Aqa Mohammadi Yuan Qin Zongshen Zhang Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
description	Konjac (<i>Amorphophallus konjac</i> K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family <i>Araceae</i>, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the plantlet regeneration efficiency of konjac using young leaves as an explant source. In the current study, we used several sterilization methods for tuber sterilization. Moreover, various plant growth regulator combinations were applied to achieve efficient somatic embryogenesis and plantlet regeneration. Our results showed that the optimal tuber sterilization was method C (75% alcohol for 15 s + 0.1% HgCl<sub>2</sub> for 15 min + washing by double-sterilized water three times). Three types of embryogenic calli were induced on full-strength Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L of 6-benzylaminopurine (6-BA), 0.5 mg/L of naphthaleneacetic acid (NAA), 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L of sucrose. Of the three types of embryogenic calli, only type Ⅲ further regenerated plantlets, with a callus induction rate of 55.73% and a seedling induction rate of 92.73%. This suggests that the addition of the above hormones gives the optimal callus induction. The proliferation rate achieved was 38% on the MS basal medium containing 1.0 mg/L of 6-BA, 1.0 mg/L of indolebutyric acid (IBA), 0.2 mg/L of kinetin (KT), and 50 g/L of sucrose. The one-step seedling formation achieved in MS medium contained 2.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0.1 mg/L of gibberellic acid (GA<sub>3</sub>), and 30 g/L of sucrose, and the number of regenerated shoots per explants was 6 ± 2. Therefore, we establish a one-step seedling regeneration system through indirect plant regeneration, which shortens the time for konjac in vitro regeneration, significantly increased the micropropagation efficiency, and decreased the cost of the konjac tissue culture.
format	article
author	Dandan Li Mohammad Aqa Mohammadi Yuan Qin Zongshen Zhang
author_facet	Dandan Li Mohammad Aqa Mohammadi Yuan Qin Zongshen Zhang
author_sort	Dandan Li
title	Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
title_short	Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
title_full	Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
title_fullStr	Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
title_full_unstemmed	Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation
title_sort	somatic embryogenesis and indirect <i>in vitro</i> plant regeneration in <i>amorphophallus konjac</i> k. koch by one-step seedling formation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c316c8b7b88147e5ae27cf8758535be4
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Somatic Embryogenesis and Indirect <i>In Vitro</i> Plant Regeneration in <i>Amorphophallus konjac</i> K. Koch by One-Step Seedling Formation

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