Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy

Abstract Studying the impact of antibiotic treatment on otitis media (OM), the leading cause of primary care office visits during childhood, is critical to develop appropriate treatment strategies. Tracking dynamic middle ear conditions during antibiotic treatment is not readily applicable in patien...

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Autores principales:	Jungeun Won, Wenzhou Hong, Pawjai Khampang, Darold R. Spillman, Samuels Marshall, Ke Yan, Ryan G. Porter, Michael A. Novak, Joseph E. Kerschner, Stephen A. Boppart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c31e0027d33d4f1e97ba8979ecd33d28
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spelling	oai:doaj.org-article:c31e0027d33d4f1e97ba8979ecd33d282021-12-02T15:53:43ZLongitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy10.1038/s41598-021-84543-92045-2322https://doaj.org/article/c31e0027d33d4f1e97ba8979ecd33d282021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84543-9https://doaj.org/toc/2045-2322Abstract Studying the impact of antibiotic treatment on otitis media (OM), the leading cause of primary care office visits during childhood, is critical to develop appropriate treatment strategies. Tracking dynamic middle ear conditions during antibiotic treatment is not readily applicable in patients, due to the limited diagnostic techniques available to detect the smaller amount and variation of middle ear effusion (MEE) and middle ear bacterial biofilm, responsible for chronic and recurrent OM. To overcome these challenges, a handheld optical coherence tomography (OCT) system has been developed to monitor in vivo response of biofilms and MEEs in the OM-induced chinchilla model, the standard model for human OM. As a result, the formation of MEE as well as biofilm adherent to the tympanic membrane (TM) was longitudinally assessed as OM developed. Various types of MEEs and biofilms in the chinchilla model were identified, which showed comparable features as those in humans. Furthermore, the effect of antibiotics on the biofilm as well as the amount and type of MEEs was investigated with low-dose and high-dose treatment (ceftriaxone). The capability of OCT to non-invasively track and examine middle ear conditions is highly beneficial for therapeutic OM studies and will lead to improved management of OM in patients.Jungeun WonWenzhou HongPawjai KhampangDarold R. SpillmanSamuels MarshallKe YanRyan G. PorterMichael A. NovakJoseph E. KerschnerStephen A. BoppartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungeun Won Wenzhou Hong Pawjai Khampang Darold R. Spillman Samuels Marshall Ke Yan Ryan G. Porter Michael A. Novak Joseph E. Kerschner Stephen A. Boppart Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
description	Abstract Studying the impact of antibiotic treatment on otitis media (OM), the leading cause of primary care office visits during childhood, is critical to develop appropriate treatment strategies. Tracking dynamic middle ear conditions during antibiotic treatment is not readily applicable in patients, due to the limited diagnostic techniques available to detect the smaller amount and variation of middle ear effusion (MEE) and middle ear bacterial biofilm, responsible for chronic and recurrent OM. To overcome these challenges, a handheld optical coherence tomography (OCT) system has been developed to monitor in vivo response of biofilms and MEEs in the OM-induced chinchilla model, the standard model for human OM. As a result, the formation of MEE as well as biofilm adherent to the tympanic membrane (TM) was longitudinally assessed as OM developed. Various types of MEEs and biofilms in the chinchilla model were identified, which showed comparable features as those in humans. Furthermore, the effect of antibiotics on the biofilm as well as the amount and type of MEEs was investigated with low-dose and high-dose treatment (ceftriaxone). The capability of OCT to non-invasively track and examine middle ear conditions is highly beneficial for therapeutic OM studies and will lead to improved management of OM in patients.
format	article
author	Jungeun Won Wenzhou Hong Pawjai Khampang Darold R. Spillman Samuels Marshall Ke Yan Ryan G. Porter Michael A. Novak Joseph E. Kerschner Stephen A. Boppart
author_facet	Jungeun Won Wenzhou Hong Pawjai Khampang Darold R. Spillman Samuels Marshall Ke Yan Ryan G. Porter Michael A. Novak Joseph E. Kerschner Stephen A. Boppart
author_sort	Jungeun Won
title	Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
title_short	Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
title_full	Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
title_fullStr	Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
title_full_unstemmed	Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
title_sort	longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c31e0027d33d4f1e97ba8979ecd33d28
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Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy

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