UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

Abstract In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. T...

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Autores principales:	Navid Assi, Lukas Nejdl, Kristyna Zemankova, Kristyna Pavelicova, Jaroslava Bezdekova, Mirek Macka, Vojtech Adam, Marketa Vaculovicova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c321341b73444ca288d91948acaa30a3
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spelling	oai:doaj.org-article:c321341b73444ca288d91948acaa30a32021-12-02T16:24:49ZUV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols10.1038/s41598-021-93137-42045-2322https://doaj.org/article/c321341b73444ca288d91948acaa30a32021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93137-4https://doaj.org/toc/2045-2322Abstract In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12–150 µg/mL was linear with a detection limit of 3.6 µg/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.Navid AssiLukas NejdlKristyna ZemankovaKristyna PavelicovaJaroslava BezdekovaMirek MackaVojtech AdamMarketa VaculovicovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Navid Assi Lukas Nejdl Kristyna Zemankova Kristyna Pavelicova Jaroslava Bezdekova Mirek Macka Vojtech Adam Marketa Vaculovicova UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
description	Abstract In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12–150 µg/mL was linear with a detection limit of 3.6 µg/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.
format	article
author	Navid Assi Lukas Nejdl Kristyna Zemankova Kristyna Pavelicova Jaroslava Bezdekova Mirek Macka Vojtech Adam Marketa Vaculovicova
author_facet	Navid Assi Lukas Nejdl Kristyna Zemankova Kristyna Pavelicova Jaroslava Bezdekova Mirek Macka Vojtech Adam Marketa Vaculovicova
author_sort	Navid Assi
title	UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
title_short	UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
title_full	UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
title_fullStr	UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
title_full_unstemmed	UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
title_sort	uv-induced zn:cd/s quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c321341b73444ca288d91948acaa30a3
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UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

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