Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat

Abstract Background Nanomaterials of biomedicine and tissue engineering have been proposed for the treatment of periodontitis in recent years. This study aimed to investigate the effects of gold nanoparticles (AuNPs) combined with human β-defensin 3 (hBD3) on the repair of the alveolar bones of expe...

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Autores principales:	Jing Zhou, Lingjun Li, Di Cui, Xiaoting Xie, Wenrong Yang, Fuhua Yan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Gold nanoparticles hBD3 Periodontitis Osteogenesis Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/c326967b54184accb6bde3960d9cbc96
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spelling	oai:doaj.org-article:c326967b54184accb6bde3960d9cbc962021-11-28T12:13:23ZEffects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat10.1186/s12938-021-00954-91475-925Xhttps://doaj.org/article/c326967b54184accb6bde3960d9cbc962021-11-01T00:00:00Zhttps://doi.org/10.1186/s12938-021-00954-9https://doaj.org/toc/1475-925XAbstract Background Nanomaterials of biomedicine and tissue engineering have been proposed for the treatment of periodontitis in recent years. This study aimed to investigate the effects of gold nanoparticles (AuNPs) combined with human β-defensin 3 (hBD3) on the repair of the alveolar bones of experimental periodontitis in rats. Methods A model of experimental periodontitis was established by ligation of the maxillary second molars with silk thread in rats, which were treated with or without AuNPs combined with hBD3. Micro‐computerized tomography (micro-CT) scanning, enzyme-linked immunosorbent assay, and histological and immunohistochemical staining, including alkaline phosphatase (ALP), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and receptor activator of NF-κB ligand (RANKL), were used to analyze the samples. Results Micro-CT demonstrated that the alveolar bone resorption was significantly reduced after the treatment with AuNPs combined with hBD3. Levels of TNF-α and IL-6 were decreased markedly compared with the ligation group. H&E and Masson staining showed that AuNPs combined with hBD3 group had less inflammatory cell infiltration, collagen fibrosis and fracture, but higher calcification in the new bone tissue. Moreover, the administration of AuNPs combined with hBD3 increased the expression levels of ALP and OPG (related to bone formation) while decreasing the expression levels of TRAP and RANKL (related to bone resorption) expression. Conclusions AuNPs combined with hBD3 had a protective effect on the progression of experimental periodontitis in rats and played a certain role in suppressing osteoclastogenesis and alleviating the inflammatory destruction of periodontitis along with the promotion of bone repair.Jing ZhouLingjun LiDi CuiXiaoting XieWenrong YangFuhua YanBMCarticleGold nanoparticleshBD3PeriodontitisOsteogenesisMedical technologyR855-855.5ENBioMedical Engineering OnLine, Vol 20, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold nanoparticles hBD3 Periodontitis Osteogenesis Medical technology R855-855.5
spellingShingle	Gold nanoparticles hBD3 Periodontitis Osteogenesis Medical technology R855-855.5 Jing Zhou Lingjun Li Di Cui Xiaoting Xie Wenrong Yang Fuhua Yan Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
description	Abstract Background Nanomaterials of biomedicine and tissue engineering have been proposed for the treatment of periodontitis in recent years. This study aimed to investigate the effects of gold nanoparticles (AuNPs) combined with human β-defensin 3 (hBD3) on the repair of the alveolar bones of experimental periodontitis in rats. Methods A model of experimental periodontitis was established by ligation of the maxillary second molars with silk thread in rats, which were treated with or without AuNPs combined with hBD3. Micro‐computerized tomography (micro-CT) scanning, enzyme-linked immunosorbent assay, and histological and immunohistochemical staining, including alkaline phosphatase (ALP), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and receptor activator of NF-κB ligand (RANKL), were used to analyze the samples. Results Micro-CT demonstrated that the alveolar bone resorption was significantly reduced after the treatment with AuNPs combined with hBD3. Levels of TNF-α and IL-6 were decreased markedly compared with the ligation group. H&E and Masson staining showed that AuNPs combined with hBD3 group had less inflammatory cell infiltration, collagen fibrosis and fracture, but higher calcification in the new bone tissue. Moreover, the administration of AuNPs combined with hBD3 increased the expression levels of ALP and OPG (related to bone formation) while decreasing the expression levels of TRAP and RANKL (related to bone resorption) expression. Conclusions AuNPs combined with hBD3 had a protective effect on the progression of experimental periodontitis in rats and played a certain role in suppressing osteoclastogenesis and alleviating the inflammatory destruction of periodontitis along with the promotion of bone repair.
format	article
author	Jing Zhou Lingjun Li Di Cui Xiaoting Xie Wenrong Yang Fuhua Yan
author_facet	Jing Zhou Lingjun Li Di Cui Xiaoting Xie Wenrong Yang Fuhua Yan
author_sort	Jing Zhou
title	Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
title_short	Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
title_full	Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
title_fullStr	Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
title_full_unstemmed	Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
title_sort	effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c326967b54184accb6bde3960d9cbc96
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Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat

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