Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events.
<h4>Background</h4>Glanders, caused by the gram-negative bacterium Burkholderia mallei, is a highly infectious zoonotic disease of solipeds causing severe disease in animals and men. Although eradicated from many Western countries, it recently emerged in Asia, the Middle-East, Africa, an...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c32a123fa9d9490a8b1960e366836ffc |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c32a123fa9d9490a8b1960e366836ffc |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c32a123fa9d9490a8b1960e366836ffc2021-11-25T06:32:00ZGenotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events.1935-27271935-273510.1371/journal.pntd.0003195https://doaj.org/article/c32a123fa9d9490a8b1960e366836ffc2014-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0003195https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Glanders, caused by the gram-negative bacterium Burkholderia mallei, is a highly infectious zoonotic disease of solipeds causing severe disease in animals and men. Although eradicated from many Western countries, it recently emerged in Asia, the Middle-East, Africa, and South America. Due to its rareness, little is known about outbreak dynamics of the disease and its epidemiology.<h4>Methodology/principal findings</h4>We investigated a recent outbreak of glanders in Bahrain by applying high resolution genotyping (multiple locus variable number of tandem repeats, MLVA) and comparative whole genome sequencing to B. mallei isolated from infected horses and a camel. These results were compared to samples obtained from an outbreak in the United Arab Emirates in 2004, and further placed into a broader phylogeographic context based on previously published B. mallei data. The samples from the outbreak in Bahrain separated into two distinct clusters, suggesting a complex epidemiological background and evidence for the involvement of multiple B. mallei strains. Additionally, the samples from Bahrain were more closely related to B. mallei isolated from horses in the United Arab Emirates in 2004 than other B. mallei which is suggestive of repeated importation to the region from similar geographic sources.<h4>Conclusion/significance</h4>High-resolution genotyping and comparative whole genome analysis revealed the same phylogenetic patterns among our samples. The close relationship of the Dubai/UAE B. mallei populations to each other may be indicative of a similar geographic origin that has yet to be identified for the infecting strains. The recent emergence of glanders in combination with worldwide horse trading might pose a new risk for human infections.Holger C ScholzTalima PearsonHeidie HornstraMichaela ProjahnRahime TerziogluRenate WerneryEnrico GeorgiJulia M RiehmDavid M WagnerPaul S KeimMarina JosephBobby JohnsonJoerg KinneShanti JoseCrystal M HeppAngela WitteUlrich WerneryPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 8, Iss 9, p e3195 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
| spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Holger C Scholz Talima Pearson Heidie Hornstra Michaela Projahn Rahime Terzioglu Renate Wernery Enrico Georgi Julia M Riehm David M Wagner Paul S Keim Marina Joseph Bobby Johnson Joerg Kinne Shanti Jose Crystal M Hepp Angela Witte Ulrich Wernery Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| description |
<h4>Background</h4>Glanders, caused by the gram-negative bacterium Burkholderia mallei, is a highly infectious zoonotic disease of solipeds causing severe disease in animals and men. Although eradicated from many Western countries, it recently emerged in Asia, the Middle-East, Africa, and South America. Due to its rareness, little is known about outbreak dynamics of the disease and its epidemiology.<h4>Methodology/principal findings</h4>We investigated a recent outbreak of glanders in Bahrain by applying high resolution genotyping (multiple locus variable number of tandem repeats, MLVA) and comparative whole genome sequencing to B. mallei isolated from infected horses and a camel. These results were compared to samples obtained from an outbreak in the United Arab Emirates in 2004, and further placed into a broader phylogeographic context based on previously published B. mallei data. The samples from the outbreak in Bahrain separated into two distinct clusters, suggesting a complex epidemiological background and evidence for the involvement of multiple B. mallei strains. Additionally, the samples from Bahrain were more closely related to B. mallei isolated from horses in the United Arab Emirates in 2004 than other B. mallei which is suggestive of repeated importation to the region from similar geographic sources.<h4>Conclusion/significance</h4>High-resolution genotyping and comparative whole genome analysis revealed the same phylogenetic patterns among our samples. The close relationship of the Dubai/UAE B. mallei populations to each other may be indicative of a similar geographic origin that has yet to be identified for the infecting strains. The recent emergence of glanders in combination with worldwide horse trading might pose a new risk for human infections. |
| format |
article |
| author |
Holger C Scholz Talima Pearson Heidie Hornstra Michaela Projahn Rahime Terzioglu Renate Wernery Enrico Georgi Julia M Riehm David M Wagner Paul S Keim Marina Joseph Bobby Johnson Joerg Kinne Shanti Jose Crystal M Hepp Angela Witte Ulrich Wernery |
| author_facet |
Holger C Scholz Talima Pearson Heidie Hornstra Michaela Projahn Rahime Terzioglu Renate Wernery Enrico Georgi Julia M Riehm David M Wagner Paul S Keim Marina Joseph Bobby Johnson Joerg Kinne Shanti Jose Crystal M Hepp Angela Witte Ulrich Wernery |
| author_sort |
Holger C Scholz |
| title |
Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| title_short |
Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| title_full |
Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| title_fullStr |
Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| title_full_unstemmed |
Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. |
| title_sort |
genotyping of burkholderia mallei from an outbreak of glanders in bahrain suggests multiple introduction events. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/c32a123fa9d9490a8b1960e366836ffc |
| work_keys_str_mv |
AT holgercscholz genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT talimapearson genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT heidiehornstra genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT michaelaprojahn genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT rahimeterzioglu genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT renatewernery genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT enricogeorgi genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT juliamriehm genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT davidmwagner genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT paulskeim genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT marinajoseph genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT bobbyjohnson genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT joergkinne genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT shantijose genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT crystalmhepp genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT angelawitte genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents AT ulrichwernery genotypingofburkholderiamalleifromanoutbreakofglandersinbahrainsuggestsmultipleintroductionevents |
| _version_ |
1718413708400525312 |