Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model

Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes...

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Autores principales:	Aleksandra Pliszczak-Król, Zdzisław Kiełbowicz, Jarosław Król, Agnieszka Antończyk, Marianna Gemra, Piotr Skrzypczak, Przemysław Prządka, Dariusz Zalewski, Janusz Bieżyński, Jakub Nicpoń
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hemostasis coagulation fibrinolysis scaffold implantation sheep Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/c33dc37c3065434ab4abf4d0385af3ef
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spelling	oai:doaj.org-article:c33dc37c3065434ab4abf4d0385af3ef2021-11-25T18:14:50ZParameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model10.3390/ma142269341996-1944https://doaj.org/article/c33dc37c3065434ab4abf4d0385af3ef2021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/6934https://doaj.org/toc/1996-1944Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery); PT and aPTT increased to 14.8 s and 33.9 s, respectively. From the third day onwards, most of these alterations began to return to normal values. The concentration of fibrinogen rose throughout the observation period (up to 8.4 g/L), mirroring the ongoing inflammatory reaction. However, no signals of significant disturbances in hemostatic processes were detected in the sheep tested.Aleksandra Pliszczak-KrólZdzisław KiełbowiczJarosław KrólAgnieszka AntończykMarianna GemraPiotr SkrzypczakPrzemysław PrządkaDariusz ZalewskiJanusz BieżyńskiJakub NicpońMDPI AGarticlehemostasiscoagulationfibrinolysisscaffold implantationsheepTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6934, p 6934 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hemostasis coagulation fibrinolysis scaffold implantation sheep Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	hemostasis coagulation fibrinolysis scaffold implantation sheep Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Aleksandra Pliszczak-Król Zdzisław Kiełbowicz Jarosław Król Agnieszka Antończyk Marianna Gemra Piotr Skrzypczak Przemysław Prządka Dariusz Zalewski Janusz Bieżyński Jakub Nicpoń Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
description	Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery); PT and aPTT increased to 14.8 s and 33.9 s, respectively. From the third day onwards, most of these alterations began to return to normal values. The concentration of fibrinogen rose throughout the observation period (up to 8.4 g/L), mirroring the ongoing inflammatory reaction. However, no signals of significant disturbances in hemostatic processes were detected in the sheep tested.
format	article
author	Aleksandra Pliszczak-Król Zdzisław Kiełbowicz Jarosław Król Agnieszka Antończyk Marianna Gemra Piotr Skrzypczak Przemysław Prządka Dariusz Zalewski Janusz Bieżyński Jakub Nicpoń
author_facet	Aleksandra Pliszczak-Król Zdzisław Kiełbowicz Jarosław Król Agnieszka Antończyk Marianna Gemra Piotr Skrzypczak Przemysław Prządka Dariusz Zalewski Janusz Bieżyński Jakub Nicpoń
author_sort	Aleksandra Pliszczak-Król
title	Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_short	Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_full	Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_fullStr	Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_full_unstemmed	Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
title_sort	parameters of hemostasis in sheep implanted with composite scaffold settled by stimulated mesenchymal stem cells—evaluation of the animal model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c33dc37c3065434ab4abf4d0385af3ef
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Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model

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