ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.

Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated...

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Autores principales:	Takeshi Tomita, Katsuaki Ieguchi, Frédéric Coin, Yasuhiro Kato, Haruhisa Kikuchi, Yoshiteru Oshima, Shoichiro Kurata, Yoshiro Maru
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c342a4b1b42b45bc9ed8827a60246ab4
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spelling	oai:doaj.org-article:c342a4b1b42b45bc9ed8827a60246ab42021-11-25T05:58:39ZZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.1932-620310.1371/journal.pone.0108957https://doaj.org/article/c342a4b1b42b45bc9ed8827a60246ab42014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108957https://doaj.org/toc/1932-6203Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated their biological functions. Our screening and subsequent pull-down assay revealed ZFC3H1 (also known as CCDC131 or CSRC2), an uncharacterized zinc finger protein, as a Celastramycin A binding protein. The knockdown of ZFC3H1 reduced IL-8 expression levels in the TNFα-stimulated lung carcinoma cell line, LU99, and UV-irradiated HeLa cells. Based on reporter assay results, we concluded that ZFC3H1 participates in the transcriptional activation of IL-8. The findings of our UV-irradiation experiments implied that ZFC3H1 may indirectly interact with ERCC1 in an activated DNA repair complex. Thus, we designated ZFC3H1 as a mammalian target of Celastramycin A (mTOC).Takeshi TomitaKatsuaki IeguchiFrédéric CoinYasuhiro KatoHaruhisa KikuchiYoshiteru OshimaShoichiro KurataYoshiro MaruPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108957 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takeshi Tomita Katsuaki Ieguchi Frédéric Coin Yasuhiro Kato Haruhisa Kikuchi Yoshiteru Oshima Shoichiro Kurata Yoshiro Maru ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
description	Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated their biological functions. Our screening and subsequent pull-down assay revealed ZFC3H1 (also known as CCDC131 or CSRC2), an uncharacterized zinc finger protein, as a Celastramycin A binding protein. The knockdown of ZFC3H1 reduced IL-8 expression levels in the TNFα-stimulated lung carcinoma cell line, LU99, and UV-irradiated HeLa cells. Based on reporter assay results, we concluded that ZFC3H1 participates in the transcriptional activation of IL-8. The findings of our UV-irradiation experiments implied that ZFC3H1 may indirectly interact with ERCC1 in an activated DNA repair complex. Thus, we designated ZFC3H1 as a mammalian target of Celastramycin A (mTOC).
format	article
author	Takeshi Tomita Katsuaki Ieguchi Frédéric Coin Yasuhiro Kato Haruhisa Kikuchi Yoshiteru Oshima Shoichiro Kurata Yoshiro Maru
author_facet	Takeshi Tomita Katsuaki Ieguchi Frédéric Coin Yasuhiro Kato Haruhisa Kikuchi Yoshiteru Oshima Shoichiro Kurata Yoshiro Maru
author_sort	Takeshi Tomita
title	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
title_short	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
title_full	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
title_fullStr	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
title_full_unstemmed	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
title_sort	zfc3h1, a zinc finger protein, modulates il-8 transcription by binding with celastramycin a, a potential immune suppressor.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c342a4b1b42b45bc9ed8827a60246ab4
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ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.

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