Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

Inhibition of digestive enzymes such as α-amylase and pancreatic lipase (PL) is a promising therapeutic strategy for the treatment and management of chronic health conditions such as diabetes and obesity. Therefore, the aim of this work was to determine the enzyme inhibitory activity of polyphenol-r...

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Autores principales:	Olayinka A. Oluwagunwa, Adeola M. Alashi, Rotimi E. Aluko
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	leaf extracts polyphenolic compounds α-amylase pancreatic lipase enzyme inhibition fluorescence intensity Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/c34514f07aba48d0b53cb2f35ca6560c
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spelling	oai:doaj.org-article:c34514f07aba48d0b53cb2f35ca6560c2021-11-08T06:36:44ZInhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves2296-861X10.3389/fnut.2021.772903https://doaj.org/article/c34514f07aba48d0b53cb2f35ca6560c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.772903/fullhttps://doaj.org/toc/2296-861XInhibition of digestive enzymes such as α-amylase and pancreatic lipase (PL) is a promising therapeutic strategy for the treatment and management of chronic health conditions such as diabetes and obesity. Therefore, the aim of this work was to determine the enzyme inhibitory activity of polyphenol-rich aqueous extracts of Amaranthus viridis (AV), Solanum macrocarpon (SM) and Telfairia occidentalis (TO) leaves, which were harvested from plants produced using multiple urea fertilizer doses (0–80 kg N/ha). Fertilizer application was applied at two time points (at planting or 2 weeks after seedling emergence). Leaf extracts were obtained using aqueous extraction (1:20, leaves:water) for 4 h at 60°C followed by centrifugation and freeze-drying of the supernatant. Results showed that the extracts inhibited α-amylase, and pancreatic lipase dose-dependently with TO extracts having significantly (p < 0.05) higher inhibitory activities for both enzymes. Fluorescence intensity and circular dichroism spectra in the presence and absence of leaf extracts indicate significant changes to the enzyme protein secondary and tertiary conformations. We conclude that the leaf extracts, especially from TO are potential agents for reducing calorie intake as a preventive or treatment tool against chronic diseases such as diabetes and obesity.Olayinka A. OluwagunwaAdeola M. AlashiRotimi E. AlukoRotimi E. AlukoFrontiers Media S.A.articleleaf extractspolyphenolic compoundsα-amylasepancreatic lipaseenzyme inhibitionfluorescence intensityNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leaf extracts polyphenolic compounds α-amylase pancreatic lipase enzyme inhibition fluorescence intensity Nutrition. Foods and food supply TX341-641
spellingShingle	leaf extracts polyphenolic compounds α-amylase pancreatic lipase enzyme inhibition fluorescence intensity Nutrition. Foods and food supply TX341-641 Olayinka A. Oluwagunwa Adeola M. Alashi Rotimi E. Aluko Rotimi E. Aluko Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
description	Inhibition of digestive enzymes such as α-amylase and pancreatic lipase (PL) is a promising therapeutic strategy for the treatment and management of chronic health conditions such as diabetes and obesity. Therefore, the aim of this work was to determine the enzyme inhibitory activity of polyphenol-rich aqueous extracts of Amaranthus viridis (AV), Solanum macrocarpon (SM) and Telfairia occidentalis (TO) leaves, which were harvested from plants produced using multiple urea fertilizer doses (0–80 kg N/ha). Fertilizer application was applied at two time points (at planting or 2 weeks after seedling emergence). Leaf extracts were obtained using aqueous extraction (1:20, leaves:water) for 4 h at 60°C followed by centrifugation and freeze-drying of the supernatant. Results showed that the extracts inhibited α-amylase, and pancreatic lipase dose-dependently with TO extracts having significantly (p < 0.05) higher inhibitory activities for both enzymes. Fluorescence intensity and circular dichroism spectra in the presence and absence of leaf extracts indicate significant changes to the enzyme protein secondary and tertiary conformations. We conclude that the leaf extracts, especially from TO are potential agents for reducing calorie intake as a preventive or treatment tool against chronic diseases such as diabetes and obesity.
format	article
author	Olayinka A. Oluwagunwa Adeola M. Alashi Rotimi E. Aluko Rotimi E. Aluko
author_facet	Olayinka A. Oluwagunwa Adeola M. Alashi Rotimi E. Aluko Rotimi E. Aluko
author_sort	Olayinka A. Oluwagunwa
title	Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
title_short	Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
title_full	Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
title_fullStr	Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
title_full_unstemmed	Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves
title_sort	inhibition of the in vitro activities of α-amylase and pancreatic lipase by aqueous extracts of amaranthus viridis, solanum macrocarpon and telfairia occidentalis leaves
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c34514f07aba48d0b53cb2f35ca6560c
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Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

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