Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats

Zhen-Yi Long,1,&ast; Yi-Feng Zhou,2,&ast; Hao Yuan,1 Ya-Meng Peng,1 Si-Xian Wu,1 Fang Peng1 1Department of Clinical Laboratory, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of China; 2Operating Room, Hunan P...

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Autores principales:	Long ZY, Zhou YF, Yuan H, Peng YM, Wu SX, Peng F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	rheumatoid arthritis immunoglobulin interleukin interleukin receptor Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c347b79fa99c4d6d9713a97a5fe8510f
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spelling	oai:doaj.org-article:c347b79fa99c4d6d9713a97a5fe8510f2021-12-02T19:16:13ZExpression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats1178-7031https://doaj.org/article/c347b79fa99c4d6d9713a97a5fe8510f2021-10-01T00:00:00Zhttps://www.dovepress.com/expression-and-correlation-of-igg4-and-il-21-in-collagen-induced-arthr-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Zhen-Yi Long,1,&ast; Yi-Feng Zhou,2,&ast; Hao Yuan,1 Ya-Meng Peng,1 Si-Xian Wu,1 Fang Peng1 1Department of Clinical Laboratory, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of China; 2Operating Room, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Hao YuanDepartment of Clinical Laboratory, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of ChinaTel +86-13677366519Fax +86-731-82278012Email yuanhao666@hunnu.edu.cnPurpose: We explored the expression levels of IgG4 and interleukin (IL)-21 in the serum and ankle joints of collagen-induced arthritis (CIA) rats at different disease stages.Materials and Methods: Wistar rats were randomly divided into normal and model groups, and the latter group was administered bovine type II collagen to induce arthritis. Enzyme-linked immunosorbent assay was performed at 21, 28, 35, and 42 days to detect IgG4 and IL-21 in the serum, followed by histological and immunohistochemical analyses of IgG4 and IL-21r in the ankle joint of rats.Results: The contents of IgG4 and IL-21 in the serum of the CIA model group were positively correlated and increased with disease progression. The expression of IgG4 and IL-21 receptors in the ankle joint of the CIA model group was significantly higher than that in the control group. These proteins were closely related to the pathological score. The serum IL-21 level in the model group was closely related to the level of IL-21 receptor in the ankle joint.Conclusion: IL-21 may promote the occurrence and development of rheumatoid arthritis by combining with IL-21r to regulate the content of IgG4.Keywords: rheumatoid arthritis, immunoglobulin, interleukin, interleukin receptorLong ZYZhou YFYuan HPeng YMWu SXPeng FDove Medical Pressarticlerheumatoid arthritisimmunoglobulininterleukininterleukin receptorPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5051-5058 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis immunoglobulin interleukin interleukin receptor Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	rheumatoid arthritis immunoglobulin interleukin interleukin receptor Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Long ZY Zhou YF Yuan H Peng YM Wu SX Peng F Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
description	Zhen-Yi Long,1,&ast; Yi-Feng Zhou,2,&ast; Hao Yuan,1 Ya-Meng Peng,1 Si-Xian Wu,1 Fang Peng1 1Department of Clinical Laboratory, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of China; 2Operating Room, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Hao YuanDepartment of Clinical Laboratory, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, People’s Republic of ChinaTel +86-13677366519Fax +86-731-82278012Email yuanhao666@hunnu.edu.cnPurpose: We explored the expression levels of IgG4 and interleukin (IL)-21 in the serum and ankle joints of collagen-induced arthritis (CIA) rats at different disease stages.Materials and Methods: Wistar rats were randomly divided into normal and model groups, and the latter group was administered bovine type II collagen to induce arthritis. Enzyme-linked immunosorbent assay was performed at 21, 28, 35, and 42 days to detect IgG4 and IL-21 in the serum, followed by histological and immunohistochemical analyses of IgG4 and IL-21r in the ankle joint of rats.Results: The contents of IgG4 and IL-21 in the serum of the CIA model group were positively correlated and increased with disease progression. The expression of IgG4 and IL-21 receptors in the ankle joint of the CIA model group was significantly higher than that in the control group. These proteins were closely related to the pathological score. The serum IL-21 level in the model group was closely related to the level of IL-21 receptor in the ankle joint.Conclusion: IL-21 may promote the occurrence and development of rheumatoid arthritis by combining with IL-21r to regulate the content of IgG4.Keywords: rheumatoid arthritis, immunoglobulin, interleukin, interleukin receptor
format	article
author	Long ZY Zhou YF Yuan H Peng YM Wu SX Peng F
author_facet	Long ZY Zhou YF Yuan H Peng YM Wu SX Peng F
author_sort	Long ZY
title	Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
title_short	Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
title_full	Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
title_fullStr	Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
title_full_unstemmed	Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats
title_sort	expression and correlation of igg4 and il-21 in collagen-induced arthritis rats
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c347b79fa99c4d6d9713a97a5fe8510f
work_keys_str_mv	AT longzy expressionandcorrelationofigg4andil21incollageninducedarthritisrats AT zhouyf expressionandcorrelationofigg4andil21incollageninducedarthritisrats AT yuanh expressionandcorrelationofigg4andil21incollageninducedarthritisrats AT pengym expressionandcorrelationofigg4andil21incollageninducedarthritisrats AT wusx expressionandcorrelationofigg4andil21incollageninducedarthritisrats AT pengf expressionandcorrelationofigg4andil21incollageninducedarthritisrats
_version_	1718376994504179712

Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats

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