LncRNA‐H19 gene plays a significant role in regulating glioma cell function

Abstract Background Glioma is an aggressive adult primary cancer, and is characterized by low cure rate, poor prognosis, and high recurrence. The present study aimed to investigate the effect of lncRNA‐H19 gene silencing on glioma cell function. Methods lncRNA‐H19 interference vector (LV3‐si‐H19) an...

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Autores principales:	Ping Liu, Xinqiong Huang, Haijun Wu, Guoling Yin, Liangfang Shen
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	animal model biological behavior Glioma cell LncRNA‐H19 interference Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c34c51f4ae104e4f8fe0dc960441c32e
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spelling	oai:doaj.org-article:c34c51f4ae104e4f8fe0dc960441c32e2021-11-10T16:39:23ZLncRNA‐H19 gene plays a significant role in regulating glioma cell function2324-926910.1002/mgg3.1480https://doaj.org/article/c34c51f4ae104e4f8fe0dc960441c32e2021-10-01T00:00:00Zhttps://doi.org/10.1002/mgg3.1480https://doaj.org/toc/2324-9269Abstract Background Glioma is an aggressive adult primary cancer, and is characterized by low cure rate, poor prognosis, and high recurrence. The present study aimed to investigate the effect of lncRNA‐H19 gene silencing on glioma cell function. Methods lncRNA‐H19 interference vector (LV3‐si‐H19) and negative control vector (LV3‐NC) were stably transfected into U251 and U87‐MG cells, respectively. Quantitative real‐time PCR (qRT‐PCR) was performed to investigate the expression of lncRNA‐H19. Cell proliferation capacity was tested by adopting cell counting kit (CCK8), and propidium iodide (PI) was used for cell cycle analysis. Meanwhile, flow cytometry (FCM) method was used to investigate cell apoptosis, cell migration capacity was detected via wound healing and transwell experiments, and sphere‐forming ability was examined in serum‐free suspension culture. Additionally, glioma animal models were conducted through injecting U251 cells to estimate the effects of lncRNA‐H19 on glioma growth in vivo. Results Knocking down lncRNA‐H19 gene could effectively suppress the proliferation of U251 and U87‐MG cells. The knockdown of lncRNA‐H19 remarkably inhibited the migration and blocked cycle progressions of U251 and U87‐MG cells, yet, no obvious changes were observed in cell apoptosis. Besides, inhibiting lncRNA‐H19 expression could attenuate sphere‐forming function of U251 and U87‐MG cells. Additionally, tumor volume and weight were significantly reduced in rats injected with U251 LV‐si‐H19 cell line compared to untransfected and negative controls, when survival time was obviously prolonged in U251 LV‐si‐H19 injection groups. Conclusion LncRNA‐H19 gene plays a carcinogenic role in glioma progression via enhancing aggressive behavior of glioma cells.Ping LiuXinqiong HuangHaijun WuGuoling YinLiangfang ShenWileyarticleanimal modelbiological behaviorGlioma cellLncRNA‐H19 interferenceGeneticsQH426-470ENMolecular Genetics & Genomic Medicine, Vol 9, Iss 10, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	animal model biological behavior Glioma cell LncRNA‐H19 interference Genetics QH426-470
spellingShingle	animal model biological behavior Glioma cell LncRNA‐H19 interference Genetics QH426-470 Ping Liu Xinqiong Huang Haijun Wu Guoling Yin Liangfang Shen LncRNA‐H19 gene plays a significant role in regulating glioma cell function
description	Abstract Background Glioma is an aggressive adult primary cancer, and is characterized by low cure rate, poor prognosis, and high recurrence. The present study aimed to investigate the effect of lncRNA‐H19 gene silencing on glioma cell function. Methods lncRNA‐H19 interference vector (LV3‐si‐H19) and negative control vector (LV3‐NC) were stably transfected into U251 and U87‐MG cells, respectively. Quantitative real‐time PCR (qRT‐PCR) was performed to investigate the expression of lncRNA‐H19. Cell proliferation capacity was tested by adopting cell counting kit (CCK8), and propidium iodide (PI) was used for cell cycle analysis. Meanwhile, flow cytometry (FCM) method was used to investigate cell apoptosis, cell migration capacity was detected via wound healing and transwell experiments, and sphere‐forming ability was examined in serum‐free suspension culture. Additionally, glioma animal models were conducted through injecting U251 cells to estimate the effects of lncRNA‐H19 on glioma growth in vivo. Results Knocking down lncRNA‐H19 gene could effectively suppress the proliferation of U251 and U87‐MG cells. The knockdown of lncRNA‐H19 remarkably inhibited the migration and blocked cycle progressions of U251 and U87‐MG cells, yet, no obvious changes were observed in cell apoptosis. Besides, inhibiting lncRNA‐H19 expression could attenuate sphere‐forming function of U251 and U87‐MG cells. Additionally, tumor volume and weight were significantly reduced in rats injected with U251 LV‐si‐H19 cell line compared to untransfected and negative controls, when survival time was obviously prolonged in U251 LV‐si‐H19 injection groups. Conclusion LncRNA‐H19 gene plays a carcinogenic role in glioma progression via enhancing aggressive behavior of glioma cells.
format	article
author	Ping Liu Xinqiong Huang Haijun Wu Guoling Yin Liangfang Shen
author_facet	Ping Liu Xinqiong Huang Haijun Wu Guoling Yin Liangfang Shen
author_sort	Ping Liu
title	LncRNA‐H19 gene plays a significant role in regulating glioma cell function
title_short	LncRNA‐H19 gene plays a significant role in regulating glioma cell function
title_full	LncRNA‐H19 gene plays a significant role in regulating glioma cell function
title_fullStr	LncRNA‐H19 gene plays a significant role in regulating glioma cell function
title_full_unstemmed	LncRNA‐H19 gene plays a significant role in regulating glioma cell function
title_sort	lncrna‐h19 gene plays a significant role in regulating glioma cell function
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/c34c51f4ae104e4f8fe0dc960441c32e
work_keys_str_mv	AT pingliu lncrnah19geneplaysasignificantroleinregulatinggliomacellfunction AT xinqionghuang lncrnah19geneplaysasignificantroleinregulatinggliomacellfunction AT haijunwu lncrnah19geneplaysasignificantroleinregulatinggliomacellfunction AT guolingyin lncrnah19geneplaysasignificantroleinregulatinggliomacellfunction AT liangfangshen lncrnah19geneplaysasignificantroleinregulatinggliomacellfunction
_version_	1718439915191009280

LncRNA‐H19 gene plays a significant role in regulating glioma cell function

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