Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.

Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) defici...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabriel Mora, Michael Scharnewski, Martin Fulda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3585416fadc4bacaade265034f0276d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3585416fadc4bacaade265034f0276d
record_format	dspace
spelling	oai:doaj.org-article:c3585416fadc4bacaade265034f0276d2021-11-18T08:10:03ZNeutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0049269https://doaj.org/article/c3585416fadc4bacaade265034f0276d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139841/?tool=EBIhttps://doaj.org/toc/1932-6203Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) deficient cells, unable to recycle FA derived from lipid deacylation, to evaluate the role of several enzymatic activities in FA trafficking and PL homeostasis in Saccharomyces cerevisiae. The data presented show that phospholipases B are not contributing to constitutive PL deacylation and are therefore unlikely to be involved in PL remodeling. In contrast, the enzymes of neutral lipid (NL) synthesis and mobilization are central mediators of FA trafficking. The phospholipid:DAG acyltransferase (PDAT) Lro1p has a substantial effect on FA release and on PL equilibrium, emerging as an important mediator in PL remodeling. The acyl-CoA dependent biosynthetic activities of NL metabolism are also involved in PL homeostasis through active modulation of the substrate available for PL synthesis. In addition TAG mobilization makes an important contribution, especially in cells from stationary phase, to FA availability. Beyond its well-established role in the formation of a storage pool, NL metabolism could play a crucial role as a mechanism to uncouple the pools of PL and acyl-CoAs from each other and thereby to allow independent regulation of each one.Gabriel MoraMichael ScharnewskiMartin FuldaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49269 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriel Mora Michael Scharnewski Martin Fulda Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
description	Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) deficient cells, unable to recycle FA derived from lipid deacylation, to evaluate the role of several enzymatic activities in FA trafficking and PL homeostasis in Saccharomyces cerevisiae. The data presented show that phospholipases B are not contributing to constitutive PL deacylation and are therefore unlikely to be involved in PL remodeling. In contrast, the enzymes of neutral lipid (NL) synthesis and mobilization are central mediators of FA trafficking. The phospholipid:DAG acyltransferase (PDAT) Lro1p has a substantial effect on FA release and on PL equilibrium, emerging as an important mediator in PL remodeling. The acyl-CoA dependent biosynthetic activities of NL metabolism are also involved in PL homeostasis through active modulation of the substrate available for PL synthesis. In addition TAG mobilization makes an important contribution, especially in cells from stationary phase, to FA availability. Beyond its well-established role in the formation of a storage pool, NL metabolism could play a crucial role as a mechanism to uncouple the pools of PL and acyl-CoAs from each other and thereby to allow independent regulation of each one.
format	article
author	Gabriel Mora Michael Scharnewski Martin Fulda
author_facet	Gabriel Mora Michael Scharnewski Martin Fulda
author_sort	Gabriel Mora
title	Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
title_short	Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
title_full	Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
title_fullStr	Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
title_full_unstemmed	Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
title_sort	neutral lipid metabolism influences phospholipid synthesis and deacylation in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c3585416fadc4bacaade265034f0276d
work_keys_str_mv	AT gabrielmora neutrallipidmetabolisminfluencesphospholipidsynthesisanddeacylationinsaccharomycescerevisiae AT michaelscharnewski neutrallipidmetabolisminfluencesphospholipidsynthesisanddeacylationinsaccharomycescerevisiae AT martinfulda neutrallipidmetabolisminfluencesphospholipidsynthesisanddeacylationinsaccharomycescerevisiae
_version_	1718422133533573120

Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.

Ejemplares similares