Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.

Toxoplasma gondii is a wide spread pathogen that can cause severe and even fatal disease in fetuses and immune-compromised hosts. As an obligate intracellular parasite, Toxoplasma must alter the environment of its host cell in order to establish its replicative niche. This is accomplished, in part,...

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Autores principales:	Mandi Wiley, Crystal Teygong, Eric Phelps, Jay Radke, Ira J Blader
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c35a998c426e413581314353b029bdf8
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spelling	oai:doaj.org-article:c35a998c426e413581314353b029bdf82021-11-18T06:56:37ZSerum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.1932-620310.1371/journal.pone.0018335https://doaj.org/article/c35a998c426e413581314353b029bdf82011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21479245/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Toxoplasma gondii is a wide spread pathogen that can cause severe and even fatal disease in fetuses and immune-compromised hosts. As an obligate intracellular parasite, Toxoplasma must alter the environment of its host cell in order to establish its replicative niche. This is accomplished, in part, by secretion of factors into the host cell that act to modulate processes such as transcription. Previous studies demonstrated that genes encoding transcription factors such as c-jun, junB, EGR1, and EGR2 were amongst the host genes that were the most rapidly upregulated following infection. In cells stimulated with growth factors, these genes are regulated by a transcription factor named Serum Response Factor. Serum Response Factor is a ubiquitously expressed DNA binding protein that regulates growth and actin cytoskeleton genes via MAP kinase or actin cytoskeletal signaling, respectively. Here, we report that Toxoplasma infection leads to the rapid activation of Serum Response Factor. Serum Response Factor activation is a Toxoplasma-specific event since the transcription factor is not activated by the closely related protozoan parasite, Neospora caninum. We further demonstrate that Serum Response Factor activation requires a parasite-derived secreted factor that signals via host MAP kinases but independently of the host actin cytoskeleton. Together, these data define Serum Response Factor as a host cell transcription factor that regulates immediate early gene expression in Toxoplasma-infected cells.Mandi WileyCrystal TeygongEric PhelpsJay RadkeIra J BladerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18335 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mandi Wiley Crystal Teygong Eric Phelps Jay Radke Ira J Blader Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
description	Toxoplasma gondii is a wide spread pathogen that can cause severe and even fatal disease in fetuses and immune-compromised hosts. As an obligate intracellular parasite, Toxoplasma must alter the environment of its host cell in order to establish its replicative niche. This is accomplished, in part, by secretion of factors into the host cell that act to modulate processes such as transcription. Previous studies demonstrated that genes encoding transcription factors such as c-jun, junB, EGR1, and EGR2 were amongst the host genes that were the most rapidly upregulated following infection. In cells stimulated with growth factors, these genes are regulated by a transcription factor named Serum Response Factor. Serum Response Factor is a ubiquitously expressed DNA binding protein that regulates growth and actin cytoskeleton genes via MAP kinase or actin cytoskeletal signaling, respectively. Here, we report that Toxoplasma infection leads to the rapid activation of Serum Response Factor. Serum Response Factor activation is a Toxoplasma-specific event since the transcription factor is not activated by the closely related protozoan parasite, Neospora caninum. We further demonstrate that Serum Response Factor activation requires a parasite-derived secreted factor that signals via host MAP kinases but independently of the host actin cytoskeleton. Together, these data define Serum Response Factor as a host cell transcription factor that regulates immediate early gene expression in Toxoplasma-infected cells.
format	article
author	Mandi Wiley Crystal Teygong Eric Phelps Jay Radke Ira J Blader
author_facet	Mandi Wiley Crystal Teygong Eric Phelps Jay Radke Ira J Blader
author_sort	Mandi Wiley
title	Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
title_short	Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
title_full	Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
title_fullStr	Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
title_full_unstemmed	Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.
title_sort	serum response factor regulates immediate early host gene expression in toxoplasma gondii-infected host cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c35a998c426e413581314353b029bdf8
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Serum response factor regulates immediate early host gene expression in Toxoplasma gondii-infected host cells.

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