Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis

Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato21Department of Frontier Medicine, 2Department of Biochemistry, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important ro...

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Autores principales:	Kazuo Yudoh, Rie Karasawa, Kayo Masuko, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c35d6a27acd244cbb856f994d7c1dc93
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spelling	oai:doaj.org-article:c35d6a27acd244cbb856f994d7c1dc932021-12-02T01:04:04ZWater-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis1176-91141178-2013https://doaj.org/article/c35d6a27acd244cbb856f994d7c1dc932009-10-01T00:00:00Zhttp://www.dovepress.com/water-soluble-fullerene-c60-inhibits-the-osteoclast-differentiation-an-a3648https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato21Department of Frontier Medicine, 2Department of Biochemistry, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the receptor activator NF&kappa;B (RANK) signal pathway required for osteoclast differentiation. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against the RANK-induced osteoclastogenesis and osteoclastic bone destruction in arthritis, both in vitro and in vivo. The effects of C60 on the RANK-induced osteoclastogenesis and osteoclastic bone resorption were examined in vitro. Adjuvant-induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 1.0 &micro;M. The left ankle joint was injected intra-articularly with water-soluble C60 (20 &micro;l) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 &micro;l) once weekly for eight weeks. Ankle joint tissues were prepared for histologic analysis. C60 significantly inhibited the responses of osteoclast precursor cells to RANK ligand, including osteoclast differentiation and osteoclastic bone resorption in vitro. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced the number of osteoclasts and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of joint destruction with time. These findings indicate that C60 downregulates the RANK-induced osteoclast differentiation and is a potential therapeutic agent for inhibition of osteoclastic bone destruction in arthritis.Keywords: fullerene, osteoclast, bone resorption arthritis, antioxidant Kazuo YudohRie KarasawaKayo Masukoet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 233-239 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kazuo Yudoh Rie Karasawa Kayo Masuko et al Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
description	Kazuo Yudoh1, Rie Karasawa1, Kayo Masuko2, Tomohiro Kato21Department of Frontier Medicine, 2Department of Biochemistry, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, JapanAbstract: Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the receptor activator NF&kappa;B (RANK) signal pathway required for osteoclast differentiation. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against the RANK-induced osteoclastogenesis and osteoclastic bone destruction in arthritis, both in vitro and in vivo. The effects of C60 on the RANK-induced osteoclastogenesis and osteoclastic bone resorption were examined in vitro. Adjuvant-induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 1.0 &micro;M. The left ankle joint was injected intra-articularly with water-soluble C60 (20 &micro;l) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 &micro;l) once weekly for eight weeks. Ankle joint tissues were prepared for histologic analysis. C60 significantly inhibited the responses of osteoclast precursor cells to RANK ligand, including osteoclast differentiation and osteoclastic bone resorption in vitro. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced the number of osteoclasts and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of joint destruction with time. These findings indicate that C60 downregulates the RANK-induced osteoclast differentiation and is a potential therapeutic agent for inhibition of osteoclastic bone destruction in arthritis.Keywords: fullerene, osteoclast, bone resorption arthritis, antioxidant
format	article
author	Kazuo Yudoh Rie Karasawa Kayo Masuko et al
author_facet	Kazuo Yudoh Rie Karasawa Kayo Masuko et al
author_sort	Kazuo Yudoh
title	Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
title_short	Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
title_full	Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
title_fullStr	Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
title_full_unstemmed	Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
title_sort	water-soluble fullerene (c60) inhibits the osteoclast differentiation and bone destruction in arthritis
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/c35d6a27acd244cbb856f994d7c1dc93
work_keys_str_mv	AT kazuoyudoh watersolublefullerenec60inhibitstheosteoclastdifferentiationandbonedestructioninarthritis AT riekarasawa watersolublefullerenec60inhibitstheosteoclastdifferentiationandbonedestructioninarthritis AT kayomasuko watersolublefullerenec60inhibitstheosteoclastdifferentiationandbonedestructioninarthritis AT etal watersolublefullerenec60inhibitstheosteoclastdifferentiationandbonedestructioninarthritis
_version_	1718403343340011520

Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis

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